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Voltage-dependent $Ca^{2+}$ Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach  

Kim, Young-Chul (Department of Physiology, Chungbuk National University, College of Medicine)
Suzuki, Hikaru (Department of Physiology, Nagoya City University Medical School)
Xu, Wen-Xie (Department of Physiology, College of Medicine, Shanghai Jiaotong University)
Hashitani, Hikaru (Department of Physiology, Nagoya City University Medical School)
Choi, Woong (Department of Pharmacology, Chungbuk National University, College of Medicine)
Yun, Hyo-Yung (Department of Surgery, Chungbuk National University, College of Medicine)
Park, Seon-Mee (Department of Internal Medicine, Chungbuk National University, College of Medicine)
Youn, Sei-Jin (Department of Internal Medicine, Chungbuk National University, College of Medicine)
Lee, Sang-Jeon (Department of Surgery, Chungbuk National University, College of Medicine)
Lee, Sang-Jin (Department of Physiology, Chungbuk National University, College of Medicine)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.12, no.6, 2008 , pp. 323-330 More about this Journal
Abstract
The properties of voltage dependent $Ca^{2+}$ current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c-Kit positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times $min^{-1}$ respectively, in the presence of $1{\mu}M$ nifedipine. Single ICC-MY isolated by enzyme treatment also showed c-Kit immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under $K^+$ -rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With $Cs^+$-rich pipette solution at $V_h=-80\;mV$, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine ($1{\mu}M$) blocked VDIC. Therefore, we successfully isolated c-Kit positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent $Ca^{2+}$ currents ($VDCC_L$).
Keywords
Gastrointestinal (GI) tract; Stomach; Interstitial cells of Cajal (ICC); Voltage-dependent $Ca^{2+}$ current (VDCC);
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