Browse > Article

Roles of ERK and NF-${\kappa}$ B in Interleukin-8 Expression in Response to Heat Shock Protein 22 in Vascular Smooth Muscle Cells  

Kang, Seung-Hun (Department of Pharmacology, School of Medicine, Pusan National University)
Lee, Ji-Hyuk (Department of Pharmacology, School of Medicine, Pusan National University)
Choi, Kyung-Ha (Department of Pharmacology, School of Medicine, Pusan National University)
Rhim, Byung-Yong (Department of Pharmacology, School of Medicine, Pusan National University)
Kim, Koan-Hoi (Department of Pharmacology, School of Medicine, Pusan National University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.12, no.4, 2008 , pp. 171-176 More about this Journal
Abstract
Heat shock proteins (HSPs) serve as molecular chaperones and play a role in cell protection from damage in response to stress stimuli. The aim of this article is to investigate whether HSP22 affects IL-8 expression in vascular smooth muscle cells (VSMCs), and which cellular factors are involved in the HSP-mediated IL-8 induction in that cell type in terms of mitogen activated protein kinase (MAPK) and transcription element. Exposure of aortic smooth muscle cells (AoSMCs) to HSP22 not only enhanced IL-8 release but also induced IL-8 transcript via promoter activation. HSP22 activated ERK and p38 MAPK in AoSMCs. HSP22-induced IL-8 release was inhibited by U0126, but not by SB202190. A mutation in the IL-8 promoter region at the binding site of NF-${\kappa}$ B, but not AP-1 or C/EBP, impaired promoter activation in response to HSP22. Delivery of I ${\kappa}$ B, but not dominant negative c-Jun, lowered HSP22-induced IL-8 release from AoSMCs. These results suggest that HS P22 induces IL-8 in VSMCs via ERK1/2, and that transcription factor NF-kB may be required for the HSP22-induced IL-8 up-regulation.
Keywords
Heat shock protein; Interleukin-8; Vascular smooth muscle cell;
Citations & Related Records

Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Aiyar N, Disa J, Ao Z, Ju H, Nerurkar S, Willette RN, Macphee CH, Johns DG, Douglas SA. Lysophosphatidylcholine induces inflammatory activation of human coronary artery smooth muscle cells. Mol Cell Biochem 295: 113-120, 2007   DOI   PUBMED
2 Brown PH, Chen TK, Birrer MJ. Mechanism of action of a dominant-negative mutant of c-Jun. Oncogene 9: 791-799, 1994   PUBMED
3 Ho FM, Kang HC, Lee ST, Chao Y, Chen YC, Huang LJ, Lin WW. The anti-inflammatory actions of LCY-2-CHO, a carbazole analogue, in vascular smooth muscle cells. Biochem Pharmacol 74: 298-308, 2007   DOI   ScienceOn
4 Inoue T, Komoda H, Nonaka M, Kameda M, Uchida T, Node K. Interleukin-8 as an independent predictor of long-term clinical outcome in patients with coronary artery disease. Int J Cardiol 124: 319-325, 2008   DOI   ScienceOn
5 Jung YD, Fan F, McConkey DJ, Jean ME, Liu W, Reinmuth N, Stoeltzing O, Ahmad SA, Parikh AA, Mukaida N. Role of P38 MAPK, AP-1, and NF-kappaB in interleukin-1beta-induced IL-8 expression in human vascular smooth muscle cells. Cytokine 18: 206-213, 2002   DOI   ScienceOn
6 Kawai T, Akira S. Toll-like receptor downstream signaling. Arthritis Res Ther 7: 12-19, 2005   DOI   PUBMED   ScienceOn
7 Rus HG, Vlaicu R, Niculescu F. Interleukin-6 and interleukin-8 protein and gene expression in human arterial atherosclerotic wall. Atherosclerosis 127: 263-271, 1996   DOI   ScienceOn
8 Steinberg D, Parthasarathy S, Carew TE, Khoo JC, Witztum JL. Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity. N Engl J Med 320, 915-924: 1989   DOI   PUBMED   ScienceOn
9 Wick G, Perschinka H, Millonig G. Atherosclerosis as an autoimmune disease: an update. Trends Immunol 22: 665-669, 2001   DOI   ScienceOn
10 Yue TL, Wang X, Sung CP, Olson B, McKenna PJ, Gu JL, Feuerstein GZ. Interleukin-8. A mitogen and chemoattractant for vascular smooth muscle cells. Circ Res 75: 1-7, 1994   DOI   PUBMED   ScienceOn
11 Pockley AG, Georgiades A, Thulin T, de Faire U, Frostegard J. Serum heat shock protein 70 levels predict the development of atherosclerosis in subjects with established hypertension. Hypertension 42: 235-238, 2003   DOI   ScienceOn
12 Wang JM, Sica A, Peri G, Walter S, Padura IM, Libby P, Ceska M, Lindley I, Colotta F. Mantovani A. Expression of monocyte chemotactic protein and interleukin-8 by cytokine-activated human vascular smooth muscle cells. Arterioscler Thromb 11: 1166-1174, 1991   DOI   PUBMED
13 Akira S, Takeda K. Toll-like receptor signalling. Nat Rev Immunol 4: 499-511, 2004   DOI   ScienceOn
14 Whitley D, Goldberg SP, Jordan WD. Heat shock proteins: a review of the molecular chaperones. J Vasc Surg 29: 748-751, 1999   DOI   ScienceOn
15 de Jong WW, Leunissen JA, Voorter CE. Evolution of the alpha-crystallin/small heat-shock protein family. Mol Biol Evol 10: 103-126, 1993   PUBMED
16 Moreau M, Brocheriou I, Petit L, Ninio E, Chapman MJ, Rouis M. Interleukin-8 mediates down-regulation of tissue inhibitor of metalloproteinase-1 expression in cholesterol-loaded human macrophages: relevance to stability of atherosclerotic plaque. Circulation 99: 420-426, 1999   DOI   PUBMED   ScienceOn
17 Wu YM, Robinson DR, Kung HJ. Signal pathways in up-regulation of chemokines by tyrosine kinase MER/NYK in prostate cancer cells. Cancer Res 64: 7311-7320, 2004   DOI   ScienceOn
18 Peveri P, Walz A, Dewald B, Baggiolini M. A novel neutrophil- activating factor produced by human mononuclear phagocytes. J Exp Med 167: 1547-1559, 1988   DOI   ScienceOn
19 Boisvert WA, Santiago R, Curtiss LK, Terkeltaub RA. A leukocyte homologue of the IL-8 receptor CXCR-2 mediates the accumulation of macrophages in atherosclerotic lesions of LDL receptor-deficient mice. J Clin Invest 101: 353-363, 1998   DOI   ScienceOn
20 Schroder JM, Christophers E. Secretion of novel and homologous neutrophil-activating peptides by LPS-stimulated human endothelial cells. J Immunol 142: 244-251, 1989   PUBMED
21 Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K. The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium. J Clin Invest 108: 1307-1314, 2001   DOI   PUBMED
22 Gerszten RE, Garcia-Zepeda EA, Lim YC, Yoshida M, Ding HA, Gimbrone MA, Jr., Luster A D, Luscinskas FW, Rosenzweig A. MCP-1 and IL-8 trigger firm adhesion of monocytes to vascular endothelium under flow conditions. Nature 398: 718-723, 1999   DOI   PUBMED   ScienceOn
23 Yang X, Coriolan D, Murthy V, Schultz K, Golenbock DT, Beasley D. Proinflammatory phenotype of vascular smooth muscle cells: role of efficient Toll-like receptor 4 signaling. Am J Physiol Heart Circ Physiol 289: H1069-1076, 2005   DOI   ScienceOn
24 MacRae TH. Structure and function of small heat shock/alpha- crystallin proteins: established concepts and emerging ideas. Cell Mol Life Sci 57: 899-913, 2000   DOI   ScienceOn
25 Pockley AG. Heat shock proteins as regulators of the immune response. Lancet 362: 469-476, 2003   DOI   ScienceOn
26 Srivastava P. Roles of heat-shock proteins in innate and adaptive immunity. Nat Rev Immunol 2, 185-194: 2002   DOI   PUBMED   ScienceOn
27 Tedgui A, Mallat, Z. Cytokines in atherosclerosis: pathogenic and regulatory pathways. Physiol Rev 86: 515-581, 2006   DOI   ScienceOn
28 Yang X, Murthy V, Schultz K, Tatro JB, Fitzgerald KA, Beasley D. Toll-like receptor 3 signaling evokes a proinflammatory and proliferative phenotype in human vascular smooth muscle cells. Am J Physiol Heart Circ Physiol 291: H2334-2343, 2006   DOI   ScienceOn
29 Perschinka H, Mayr M, Millonig G, Mayerl C, van der Zee R, Morrison SG, Morrison RP, Xu Q, Wick, G. Cross-reactive B-cell epitopes of microbial and human heat shock protein 60/65 in atherosclerosis. Arterioscler Thromb Vasc Biol 23: 1060-1065, 2003   DOI   ScienceOn