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Characterization of Ionic Currents in Human Neural Stem Cells  

Lim, Chae-Gil (Department of Physiology, College of Medicine, Dankook University, Department of Physical Therapy, Gachon University of Medicine and Science Zucheon)
Kim, Sung-Soo (Department of Anatomy, Ajou University School of Medicine)
SuhKim, Hae-Young (Department of Anatomy, Ajou University School of Medicine)
Lee, Young-Don (Department of Anatomy, Ajou University School of Medicine)
Ahn, Seung-Cheol (Department of Physiology, College of Medicine, Dankook University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.12, no.4, 2008 , pp. 131-135 More about this Journal
Abstract
The profile of membrane currents was investigated in differentiated neuronal cells derived from human neural stem cells (hNSCs) that were obtained from aborted fetal cortex. Whole-cell voltage clamp recording revealed at least 4 different currents: a tetrodotoxin (TTX)-sensitive $Na^+$ current, a hyperpolarization-activated inward current, and A-type and delayed rectifier-type $K^+$ outward currents. Both types of $K^+$ outward currents were blocked by either 5 mM tetraethylammonium (TEA) or 5 mM 4-aminopyridine (4-AP). The hyperpolarization-activated current resembled the classical $K^+$ inward current in that it exhibited a voltage-dependent block in the presence of external $Ba^{2+}$ (30 ${\mu}$M) or $Cs^+$ (3${\mu}$M). However, the reversal potentials did not match well with the predicted $K^+$ equilibrium potentials, suggesting that it was not a classical $K^+$ inward rectifier current. The other $Na^+$ inward current resembled the classical $Na^+$ current observed in pharmacological studies. The expression of these channels may contribute to generation and repolarization of action potential and might be regarded as functional markers for hNSCs-derived neurons.
Keywords
Human neural stem cells; TTX-sensitive $Na^+$ current; A-type; delayed rectifier; hyperpolarization-activated inward current;
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