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Effects of TGF-${\beta}1$ Ribbon Antisense on $CCl_4$-induced Liver Fibrosis  

Doh, Kyung-Oh (Department of Physiology, College of Medicine, Dongguk University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.12, no.1, 2008 , pp. 1-6 More about this Journal
Abstract
Ribbon-type antisense oligonucleotide to TGF-${\beta}1$ (TGF-${\beta}1$ RiAS) was designed and tested to prevent or resolve the fibrotic changes induced by $CCl_4$ injection. When Hepa1c1c7 cells were transfected with TGF-${\beta}1$ RiAS, the level of TGF-${\beta}1$ mRNA was effectively reduced. TGF-${\beta}1$ RiAS, mismatched RiAS, and normal saline were each injected to mice via tail veins. When examined for the biochemical effects on the liver, TGF-${\beta}1$ mRNA levels were significantly reduced only in the TGF-${\beta}1$ RiAS-treated group. The results of immunohistochemical studies showed that TGF-${\beta}1$ RiAS prevented the accumulation of collagen and ${\alpha}$-smooth muscle actin, but could not resolve established fibrosis. These results indicate that ribbon antisense to TGF-${\beta}1$ with efficient uptake can effectively prevent fibrosis of the liver.
Keywords
Transforming growth factor-${\beta}1$; Liver cirrhosis; Ribbon antisense; Cationic peptide;
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