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Effects of Nitric Oxide on Inhibitory Receptors of Rod Bipolar Cells of Rat Retina  

Park, No-Gi (Deportment of Physics add Biophysics, The Catholic University of Korea)
Bai, Sun-Ho (Deportment of Physics add Biophysics, The Catholic University of Korea)
Jung, Chang-sub (Deportment of Physics add Biophysics, The Catholic University of Korea)
Chun, Mynng-Hoon (Deportment of Anatomy, College of Medicine, The Catholic University of Korea)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.9, no.6, 2005 , pp. 347-352 More about this Journal
Abstract
The effects of nitric oxide (NO) on inhibitory neurotransmitter receptors and some types of inhibitory receptors in dissociated rod bipolar cell (RBC) were investigated. In the whole cell voltage-clamping mode, the gamma-aminobutyric acid (GABA) activated current showed both sustained and transient components. GABA activated transient current was fully blocked by bicuculine, a $GABA_A$ receptor antagonist. The cis-4-aminocrotonic acid (CACA), a $GABA_C$ receptor agonist, evoked the sustained current that was not blocked by bicuculline (BIC). Glycine activated the transient current. These results indicate that the RBCs possess $GABA_A$, $GABA_C$, and glycine inhibitory receptors. Sodium nitroprusside (SNP), a NO analogue, reduced the currents activated by $GABA_A$ receptor only, however, did not reduce the currents activated by either $GABA_C$ or glycine receptors. This study signifies further that only NO depresses the fast inhibitory response activated by $GABA_A$ receptor in RBC. We, therefore, postulate that NO might depress the light-on/off transient inhibitory responses in RBCs in the rat retina.
Keywords
Nitric oxide; Sodium nitroprusside; GABA receptor; Glycine receptor; Whole-cell Patch-clamp; Rod bipolar cells; Retina;
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