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YS 49, a Synthetic Isoquinoline Alkaloid, Protects Sheep Pulmonary Artery Endothelial Cells from tert-butylhydroperoxide-mediated Cytotoxicity  

Chong, Won-Seog (Department of Pharmacology, College of Medicine, Seonam University)
Kang, Sun-Young (Department of Pharmacology, College of Medicine, and Institute of Health Science, Gyeongsang National University)
Kang, Young-Jin (Department of Pharmacology, College of Medicine Yeungnam University)
Park, Min-Kyu (Department of Pharmacology, College of Medicine, and Institute of Health Science, Gyeongsang National University)
Lee, Young-Soo (Department of Pharmacology, College of Medicine, and Institute of Health Science, Gyeongsang National University)
Kim, Hye-Jung (Department of Pharmacology, College of Medicine, and Institute of Health Science, Gyeongsang National University)
Seo, Han-Geuk (Department of Pharmacology, College of Medicine, and Institute of Health Science, Gyeongsang National University)
Lee, Jae-Heun (Department of Pharmacology, College of Medicine, and Institute of Health Science, Gyeongsang National University)
ChoiYun, Hye-Sook (Natural Products Research Institute, Seoul National University)
Chang, Ki-Churl (Department of Pharmacology, College of Medicine, and Institute of Health Science, Gyeongsang National University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.9, no.5, 2005 , pp. 283-289 More about this Journal
Abstract
Endothelium, particularly pulmonary endothelium, is predisposed to injury by reactive oxygen species (ROS) and their derivatives. Heme oxygenase (HO) has been demonstrated to provide cytoprotective effects in models of oxidant-induced cellular and tissue injuries. In the present study, we investigated the effects of YS 49 against oxidant [tert-butylhydroperoxide (TBH)]-induced injury using cultured sheep pulmonary artery endothelial cells (SPAECs). The viability of SPAECs was determined by quantifying reduction of a fluorogenic indicator Alamar blue. We found that TBH decreased cell viability in a timeand concentration-dependent manner. YS 49 concentration- and time-dependently increased HO-1 induction on SPAECs. As expected, YS 49 significantly decreased the TBH-induced cellular injury. In the presence of zinc protophorphyrin, HO-1 inhibitor, effect of YS 49 was significantly inhibited, indicating that HO-1 plays a protective role for YS 49. Furthermore, YS 49 showed free radical scavenging activity as evidenced by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and inhibition of lipid peroxidation. However, YS 49 did not inhibit apoptosis induced by lipopolysaccharide (LPS) in SPAECs. Taken together, HO-1 induction along with strong antioxidant action of YS 49 may be responsible for inhibition of TBH-induced injury in SPAECs.
Keywords
Reactive oxygen species; Sheep pulmonary artery endothelial cells; Heme oxygenase; YS 49; Antioxidant;
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1 Chan EL, Murphy JT. Reactive oxygen species mediate endotoxininduced human dermal endothelial NF-kappaB activation. J Surg Res 111: 120-126, 2003   DOI   ScienceOn
2 Fisher AB, Dodia C, Tan Z, Ayene I, Eckenhoff RG. Oxygendependent lipid peroxidation during lung ischemia. J Clin Invest 88: 674-679, 1991   DOI   PUBMED
3 Greenlund LJ, Deckwerth TL, Johnson EM Jr. Superoxide dismutase delays neuronal apoptosis: a role for reactive oxygen species in programmed neuronal death. Neuron 14: 303-315, 1995   DOI   ScienceOn
4 Louie S, Halliwell B, Cross C. Adult respiratory distress syndrome: a radical perspective. Adv Pharmacol 38: 457-490, 1997   PUBMED
5 Motterlini R, Foresti R, Intaglietta M, Winslow RM. NO-mediated activation of heme oxygenase: endogenous cytoprotection against oxidative stress to endothelium. Am J Physiol 270: H107-H114, 1996   PUBMED
6 Otterbein LE, Choi AMK. Heme oxygenase: colors of defense against cellular stress. Am J Physiol Lung Cell Mol Physiol 279: L1029-L1037, 2000   PUBMED
7 Applegate LA, Luscher P, Tyrrell RM. Induction of heme oxygenase: a general response to oxidant stress in cultured mammalian cells. Cancer Res 51: 974-978, 1991   PUBMED
8 Balogun E, Hoque M, Gong P, Killeen E, Green CJ, Foresti R, Alam J, Motterlini R. Curcumin activates the haem oxygenase-1 gene via regulation of Nrf2 and the antioxidant-responsive element. Biochem J 371: 887-995, 2003   DOI   ScienceOn
9 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254, 1976   DOI   PUBMED   ScienceOn
10 Haimovitz-Friedman A, Balaban N, McLoughlin M, Ehleiter D, Michaeli J, Vlodavsky I, Fuks Z. Protein kinase C mediates basic fibroblast growth factor protection of endothelial cells against radiation-induced apoptosis. Cancer Res 54: 2591-2597, 1994   PUBMED
11 Matsubara T, Ziff M. Increased superoxide anion release from human endothelial cells in response to cytokines. J Immunol 137: 3295-3298, 1986   PUBMED
12 Zweier JL, Kuppusamy P, Lutty GA. Measurement of endothelial cell free radical generation: evidence for a central mechanism of free radical injury in postischemic tissues. Proc Natl Acad Sci (USA) 85: 4046-4050, 1988   DOI   ScienceOn
13 Brigham K, Meyrick B. Endotoxin and lung injury. Am Rev Respir Dis 133: 913-927, 1986   PUBMED
14 Yun-Choi HS, Pyo MK, Park KM, Chang KC, Lee DH. Antithrombotic effects of YS-49 and YS-51--1-naphthylmethyl analogs of higenamine. Thromb Res 104: 249-255, 2001   DOI   ScienceOn
15 McCord JM. Human disease, free radicals and oxidant/antioxidant balance. Clin Biochem 26: 351-357, 1993   DOI   PUBMED   ScienceOn
16 Abraham NG., Lavrovsky Y, Schwartzman ML, Stoltz RA, Levere RD, Gerritsen ME, Shibahara S, Kappas A. Transfection of the human heme oxygenase gene into rabbit coronary microvessel endothelial cells: protective effect against heme and hemoglobin toxicity. Proc Natl Acad Sci (USA) 92: 6798-6802, 1995   DOI   ScienceOn
17 Holland JA, Pritchard KA, Pappolla MA, Wolin MS, Rogers NJ, Stemerman MB. Bradykinin induces superoxide anion release from human endothelial cells. J Cell Physiol 143: 21-25, 1990   DOI   PUBMED
18 Lee YS, Kim CH, Yun-Choi HS, Chang KC. Cardiovascular effect of a naphthylmethyl substituted tetrahydroisoquinoline, YS 49, in rat and rabbit. Life Sci 55: PL415-PL420, 1994   DOI   ScienceOn
19 Leff J, Bayer J, Bodman M, Kirkman J, Stanley P, Patton L, Beehler C, McCord J, Repine J. Interleukin-1-induced lung neutrophil accumulation and oxygen metabolite-induced lung leak in rats. Am J Physiol 266: L2-L8, 1994   PUBMED
20 Polunovsky VA, Wendt CH, Ingbar DH, Peterson MS, Bitterman PB. Induction of endothelial cell apoptosis by TNF alpha: modulation by inhibitors of protein synthesis. Exp Cell Res 214: 584- 594, 1994   DOI   PUBMED   ScienceOn
21 Kang YJ, Koo EB, Lee YS, Yun-Choi HS, Chang KC. Prevention of the expression of inducible nitric oxide synthase by a novel positive inotropic agent, YS 49, in rat vascular smooth muscle and RAW 264.7 macrophages. Br J Pharmacol 128: 357-364, 1999   DOI   ScienceOn
22 Choi AM, Alam J. Heme oxygenase-1: function, regulation, and implication of a novel stress-inducible protein in oxidant-induced lung injury. Am J Respir Cell Mol Biol 15: 9-19, 1996   DOI   PUBMED   ScienceOn
23 Cos P, Rajan P, Vedernikova I, Calomme M, Pieters L, Vlietinck AJ, Augustyns K, Haemers A, Vanden Berghe D: In vitro antioxidant profile of phenolic acid derivatives. Free Radic Res 36: 711-716, 2002   DOI   ScienceOn
24 Hoyt DG, Mannix RJ, Rusnak JM, Pitt BR, Lazo JS. Collagen is a survival factor against LPS-induced apoptosis in cultured sheep pulmonary artery endothelial cells. Am J Physiol 269: L171-L177, 1995   PUBMED
25 Terry CM, Clikeman JA, Hoidal JR, Callahan KS. Effect of tumor necrosis factor-alpha and interleukin-1 alpha on heme oxygenase- 1 expression in human endothelial cells. Am J Physiol 274: H883-H891, 1998   PUBMED
26 Kronke G, Bochkov VN, Huber J, Gruber F, Bluml S, Furnkranz A, Kadl A, Binder BR, Leitinger N. Oxidized phospholipids induce expression of human heme oxygenase-1 involving activation of cAMP-responsive element-binding protein. J Biol Chem 278: 51006-51014, 2003   DOI   ScienceOn
27 Maines MD. The heme oxygenase system: a regulator of second messenger gases. Annu Rev Pharmacol Toxicol 37: 517-554, 1997   DOI   PUBMED   ScienceOn
28 Esterbauer H, Zollner H. Methods for determination of aldehydic lipid peroxidation products. Free Rad Biol Med 7: 197-203, 1989   DOI   ScienceOn
29 Frey EA, Finlay BB. Lipopolysaccharide induces apoptosis in bovine endothelial cell line via a soluble CD 14 dependent pathway. Micob Pathog 2: 101-109, 1988
30 Freeman B, Crapo J. Hyperoxia increases oxygen radical production in rat lungs and lung mitochondria. J Biol Chem 256: 10986- 10992, 1981   PUBMED
31 King N, McGivan JD, Griffiths EJ, Halestrap AP, Suleiman MS. Martinon F, Burns K, Tschopp J. The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta. Mol Cell 10: 417-426, 2002   DOI   ScienceOn
32 Martinon F, Burns K, Tschopp J. The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta. Mol Cell 10: 417-426, 2002   DOI   ScienceOn
33 Choi KB, Wong F, Harlan JM, Chaudhary PM, Hood L, Karsan A. Lipopolysaccharide mediates endothelial apoptosis by a FADD-dependent pathway. J Biol Chem 273: 20185-20188, 1998   DOI   ScienceOn
34 McCoubrey WK Jr, Huang TJ, Maines MD. Isolation and characterization of a cDNA from the rat brain that encodes hemoprotein heme oxygenase-3. Eur J Biochem 247: 725-732, 1997   DOI   ScienceOn
35 Sanders SP, Zweier JL, Kuppusamy P, Harrison SJ, Basset DJP, Gabrielson EW, Sylvester JT. Hyperoxic sheep pulmonary microvascular endothelial cells generate free radicals via mitochondrial electron transport. J Clin Invest 91: 46-52, 1993   DOI   PUBMED