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Caffeic Acid Phenethyl Ester Inhibits the PKC-Induced IL-6 Gene Expression in the Synoviocytes of Rheumatoid Arthritis Patients  

Hur, Gang-Min (Department of Pharmacology, College of Medicine, Chungnam National University)
Hwang, Yin-Bang (Department of Pharmacology, College of Medicine, Chungnam National University)
Lee, Jae-Heun (Department of Pharmacology, College of Medicine, Chungnam National University)
Bae, So-Hyun (Department of Pharmacology, College of Medicine, Chungnam National University)
Park, Ji-Sun (Department of Pharmacology, College of Medicine, Chungnam National University)
Lee, Choong-Jae (Department of Pharmacology, College of Medicine, Chungnam National University)
Seok, Jeong-Ho (Department of Pharmacology, College of Medicine,Cancer Research Institute, Chungnam National University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.7, no.6, 2003 , pp. 363-368 More about this Journal
Abstract
To gain insight on the role of pro-inflammatory cytokines in the pathogenesis and treatment of rheumatoid arthritis (RA), the phorbol 12-myristate 13-acetate (PMA)-induced IL-6 gene expression and the effect of caffeic acid phenethyl ester (CAPE) on the PMA-induced IL-6 gene expression were investigated in human fibroblast-like synoviocytes (FLSs). Synovial tissue samples were obtained from rheumatoid arthritis patients, and FLSs were isolated. The cells were stimulated with PMA (100 nM) for 6 hrs to induce IL-6 gene. The cells were pretreated with CAPE (20, 50, $100{\mu}M$) prior to PMA treatment. PMA increased IL-6 RNA expression, binding activities of transcription factors ($NF-{\kappa}B$, AP-1) to IL-6 promoter, and IL-6 promoter activity. However, CAPE inhibited PMA-induced IL-6 mRNA expression in dose-dependent manner, and also inhibited the increased binding activities of transcription factors to IL-6 promoter and IL-6 promoter activity. These results suggest that CAPE might regulate PKC-mediated IL-6 expression and inflammatory reactions in RA.
Keywords
IL-6 gene expression; Phorbol ester; Caffeic acid phenethyl ester; Synoviocytes; Rheumatoid arthritis;
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