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Enhancement of ATP-induced Currents by Phospholipase D1 Overexpressed in PC12 Cells  

Park, Jin-Bong (Departments of Physiology, College of Medicine, Chungnam National University)
Kim, Young-Rae (Departments of Biochemistry, College of Medicine, Chungnam National University)
Jeon, Byeong-Hwa (Departments of Physiology, College of Medicine, Chungnam National University)
Park, Seung-Kiel (Departments of Biochemistry, College of Medicine, Chungnam National University)
Oh, Sae-Ock (Departments of Biochemistry, College of Medicine, Chungnam National University)
Kim, Young-Geun (Departments of Physiology, College of Medicine, Chungnam National University)
Lee, Sang-Do (Departments of Physiology, College of Medicine, Chungnam National University)
Kim, Kwang-Jin (Departments of Physiology, College of Medicine, Chungnam National University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.7, no.4, 2003 , pp. 223-229 More about this Journal
Abstract
Using phospholipase D1 (PLD1)-overexpressing PC12 (PLD1-PC12) cells, the regulatory roles of PLD1 on ATP-induced currents were investigated. In control and PLD1-PC12 cells, ATP increased PLD activity in an external $Ca^{2+}$ dependent manner. PLD activity stimulated by ATP was substantially larger in PLD1-PC12 cells than in control cells. In whole-cell voltage-clamp mode, ATP induced transient inward and outward currents. The outward currents inhibited by TEA or charybdotoxin were significantly larger in PLD1-PC12 cells than in control cells. The inward currents known as $Ca^{2+}$ permeable nonselective cation currents were also larger in PLD1-PC12 cells than in control cells. However, the difference between the two groups of cells disappeared in $Ca^{2+}$-free external solution, where ATP did not activate PLD. Finally, ATP-induced $^{45}Ca$ uptakes were also larger in PLD1-PC12 cells than in control cells. These results suggest that PLD enhances ATP-induced $Ca^{2+}$ influx via $Ca^{2+}$ permeable nonselective cation channels and increases subsequent $Ca^{2+}$-activated $K^+$ currents in PC12 cells.
Keywords
Phospholipase D1; ATP-induced currents; PC12 cell;
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