Browse > Article

Effects of Chlorhexidine Digluconate on Rotational Rate of n-(9-Anthroyloxy)stearic Acid in Porphyromonas ginginvalis Outer Membranes  

Jang, Hye-Ock (Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Cha, Seong-Kweon (Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Lee, Chang (Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Choi, Min-Gak (Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Huh, Sung-Ryul (Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Shin, Sang-Hun (Department of Oral and Maxillofacial Surgery and Clinical Pharmacology, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Chung, In-Kyo (Department of Oral and Maxillofacial Surgery and Clinical Pharmacology, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Yun, Il (Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.7, no.3, 2003 , pp. 125-130 More about this Journal
Abstract
The aim of this study was to provide a basis for studying the molecular mechanism of pharmacological action of chlorhexidine digluconate. Fluorescence polarization of n-(9-anthroyloxy)stearic acid was used to examine the effect of chlorhexidine digluconate on differential rotational mobility of different positions of the number of membrane bilayer phospholipid carbon atoms. The six membrane components differed with respect to 2, 3, 6, 9, 12, and 16-(9-anthroyloxy)stearic acid (2-AS, 3-AS, 6-AS, 9-AS, 12-AS and 16-AP) probes, indicating different membrane fluidity. Chlorhexidine digluconate increased the rate of rotational mobility of hydrocarbon interior of the cultured Porphyromonas gingivalis outer membranes (OPG) in a dose-dependent manner, but decreased the mobility of surface region (membrane interface) of the OPG. Disordering or ordering effects of chlorhexidine digluconate on membrane lipids may be responsible for some, but not all of its bacteriostatic and bactericidal actions.
Keywords
Bacterial outer membranes; Chlorhexidine digulconate; Fluorescence probe technique; Membrane hydrocarbon interior; Membrane surface; Rotational rate; n-(9-Anthroyloxy) stearic acid;
Citations & Related Records

Times Cited By SCOPUS : 1
연도 인용수 순위
1 Fisher RG, Quintana RP, Boulware MA. Surface-chemical studies on chlorhexidine and related compounds. I. Effects at air-water, n-hexane-water, and hydroxyapatite-water interfaces. J Dent Res 54: 20-24, 1975   DOI   ScienceOn
2 Fisher RG, Quintana RP. Surface-chemical studies on chlorhexidine and related compounds. II. Interaction with monomolecular-film systems. J Dent Res 54: 25-31, 1975   DOI   ScienceOn
3 Gabler WL, Roberts D, Harold W. The effect of chlorhexidine on blood cells. J Periodontal Res 22: 150-155, 1987   DOI
4 Kenney EB, Saxe SR, Bosles RD. Effect of chlorhexidine on human polymorphonuclear leukocytes. Arch Oral Biol 17: 1633-1636, 1972   DOI   ScienceOn
5 Lamont RJ, Chan A, Belton CM, Izutsu KT, Vasel D, Weinbero A. Porphyromonas gingivalis invasion of gingival epithelial cells. Infect Immun 63: 3878-3885, 1995
6 Mason JT. Properties of phosphatidylcholine bilayers as revealed by mixed-acyl phospholipid fluorescent probes containing n- (9-anthroyloxy) fatty acids. Biochim Biophys Acta 1194: 99-108, 1994   DOI   ScienceOn
7 Molitoris BA, Hoilien C. Static and dynamic components of renal cortical brush border and basolateral membrane fluidity: Role of cholesterol. J Membrane Biol 99: 165-172, 1987   DOI
8 Smalley JW, Birss AJ. Trypsin-like enzyme activity of the extracellular vesicles of Bacteroides gingivalis W50. J Gen Microbiol 133: 2883-2894, 1987
9 Tsutsui H, Kinouchi T, Wakano Y, Ohnishi Y. Purification and characterization of a protease from Bacteroides gingivalis 381. Infect Immun 55: 420-427, 1987
10 Lowry OH, Rosebrough NR, Farr AL, Randall RJ. Protein measurement with the olin phenol reagent. J Biol Chem 193: 265-275, 1951
11 Newbrun E. Anticaries and antiplaque/anti-gingivitis agents. In Neidle EA, Yagiela JA ed, Pharmacology and Therapeutics for Dentistry. The C.V. Mosby Co., Philadelphia, pp 603-624, 1989
12 Thulborn KR, Tilley LM, Sawyer WH, Treloar FE. The use of n-(9-anthroyloxy) fatty acids to determine fluidity and polarity gradients in phospholipid bilayers. Biochim Biophys Acta 558: 166-178, 1979   DOI   ScienceOn
13 Tsuchiya H. Effects of green tea catechins on membrane fluidity. Pharmacol 59: 34-44, 1999   DOI   ScienceOn
14 Gerlach RW, White DJ. Removal of extrinsic using a tartar control whitening dentifrice: a randomizied clinical trial. J Clin Dent 12: 42-46, 2001
15 LÖe H, Schiott Rindom C. The effect of mouthrinses and topical applications of chlorhexidine on the development of dental plaque and gingivitis in man. J Periodontal Res 5: 79-83, 1970   DOI
16 Russell AD. Chlorhexidine: Antibacterial action and bacterial resistance. Infection 14: 212-215, 1986   DOI
17 Ferretti GS, Hansen IA, Whittenburg K, Brown AT, Lillich TT, Ash RC. Therapeutic use of chlorhexidine in bone marrow transplant patients: case studies. Oral Med Oral Surg Oral Pathol 63: 683- 687, 1987b   DOI   ScienceOn
18 Tilley L, Thulborn KR, Sawyer WH. As assessment of the fluidity gradient of the lipid bilayer as determined by a set of n-(9- anthroyloxy) fatty acids (n=2,6,9,12,16). J Biol Chem 254: 2592- 2594, 1979
19 Buck RA, Eleazer PD, Staat RH, Scheetz JP. Effectiveness of three endodontic irrigants at various tubular depths in human dentin. J Endod 27: 206-208, 2001   DOI   ScienceOn
20 Ferretti GS, Ash RC, Brown AT, Largent BM, Kaplan A, Lillich TT. Chlorhexidine for prophylaxis against oral infections and associated complications in patients receiving bone marrow transplants. J Am Dent Assoc 114: 461-467, 1987a   DOI
21 Abrams FS, London E. Extension of parallax analysis of membrane penetration depth to the polar region of model membranes: Use of fluorescence quenching by a spin-label attached to the phospholipid polar headgroup. Biochemistry 32: 10826-10831, 1993   DOI   ScienceOn
22 Spratt DA, Pratten J, Wilson M, Gulablvala K. An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates. Int Endod J 34: 300-307, 2001   DOI   ScienceOn
23 Audus KL, Tavakoli-Saberi MR, Zheng H, Boyce EN. Chlorhexidine effects on membrane lipid domains of human buccal epithelial cells. J Dent Res 71: 1298-1303, 1992   DOI   ScienceOn
24 Hugo WB. Membrane-active antimicrobial compounds-a reappraisal of their mode of action in the light of the chemiosmotic theory. Int J Pharm 1: 127-131, 1978   DOI   ScienceOn
25 Villalaín J, Prieto M. Location and interaction of N-(9-anthroyloxy)- stearic acid probes incorporated in phosphatidylcholine vesicles. Chem Phys Lipids 59: 9-16, 1991   DOI   ScienceOn
26 Knuutilla M, SÖdering E. Effect of chlorhexidine on the release of lysosomal enzymes from cultured macrophages. Acta Odontologica Scandinavica 39: 285-289, 1981   DOI   ScienceOn
27 Schachter D. Fluidity and function of hepatocyte plasma membranes. Hepatology 4: 140-151, 1984   DOI
28 Smalley JW, Birss AJ, Mckee AS, Marsh PD. Haemin-binding proteins of Porphyromonas gingivalis W50 grown in a chemostat under haemin-limitation. J Gen Microbiol 139: 2145-2150, 1993   DOI   PUBMED   ScienceOn
29 Stubbs CD, Rubin E. Molecular mechanism of ethanol and anesthetic actions: lipid- and protein-based theories. In Alling C, Diamond I, Leslie SW, Sun GY, Wood WG ed. Alcohol, Cell Membranes, and Signal Transduction in Brain, Pleum Press, New York, pp 1-11, 1993
30 Abrams FS, Chattopadhyay A, London E. Determination of the location of fluorescent probes attached to fatty acids using parallax analysis of fluorescence quenching: effect of carboxyl ionization state and environment on depth. Biochemistry 31: 5322-5327, 1992   DOI   ScienceOn
31 Molitoris BA, Alfery AC, Arris RA, Simon FR. Renal apical membrane cholesterol and fluidity in regulation of phosphate transport. Am J Physiol 249: 12-19, 1985
32 Vincent M, De Furesta D, Gallay J, Alfsen A. Fluorescence anisotropy decays of n-(9-anthroyloxy) fatty acids in dipalmitoyl phosphatidylcholine vesicles: Localization of the effects of cholesterol addition. Biochim Biophys Res Commun 107: 914- 921, 1982   DOI   ScienceOn