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Altered Secretory Pattern of Pancreatic Enzymes and Gastrointestinal Hormones in Streptozotocin-induced Diabetic Rats  

Kim, Myung-Jun (Department of Physiology, College Of Medicine, The Catholic University of Korea)
Ryu, Gyeong-Ryul (Department of Physiology, College Of Medicine, The Catholic University of Korea)
Yi, Sae-Young (Department of Physiology, College Of Medicine, The Catholic University of Korea)
Min, Do-Sik (Department of Physiology, College Of Medicine, The Catholic University of Korea)
Rhie, Duck-Joo (Department of Physiology, College Of Medicine, The Catholic University of Korea)
Yoon, Shin-Hee (Department of Physiology, College Of Medicine, The Catholic University of Korea)
Hahn, Sang-June (Department of Physiology, College Of Medicine, The Catholic University of Korea)
Kim, Myung-Suk (Department of Physiology, College Of Medicine, The Catholic University of Korea)
Jo, Yang-Hyeok (Department of Physiology, College Of Medicine, The Catholic University of Korea)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.6, no.6, 2002 , pp. 311-317 More about this Journal
Abstract
This study was performed to investigate the pancreatic exocrine dysfunction in streptozotocin- induced diabetic rats. Changes in pancreatic enzymes secretion and in pancreatic enzymes content were observed. The output and the tissue content of amylase were significantly reduced in diabetic rats, while the output and the content of lipase were increased. Plasma secretin and cholecystokinin (CCK) concentrations of diabetic rats were significantly increased compared to those of normal rats. The altered pancreatic exocrine function was abolished by the exogenous insulin administration. The exogenous insulin also restored the increased plasma secretin and CCK concentrations. From the above results, it is suggested that, in streptozotocin-induced diabetic rats, anticoordinated changes in pancreatic enzymes secretion as well as pancreatic enzymes content are attributable to insulin deficiency and that the insulin deficiency is responsible for the increased plasma concentrations of both secretin and CCK. However, it is not clear whether the elevated plasma secretin and CCK concentrations played a direct role in changes of pancreatic exocrine function.
Keywords
Amylase; Lipase; Secretin; Cholecystokinin; Streptozotocin; Diabetes;
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1 Chey WY, Shay H, Schuman CR. External pancreatic secretion in diabetes mellitus. Ann Intern Med 59: 812－821, 1963   DOI   PUBMED   ScienceOn
2 Domschke S, Bloom SR, Adrian TE, Lux G, Domschke W. Chronic pancreatitis and diabetes mellitus: plasma and gastroduodenal mucosal profiles of regulatory peptides. Hepato-Gastroenterology 35: 229－237, 1988   PUBMED
3 Duan RD, Wicker C, Erlanson-Albertsson C. Effect of insulin administration on contents, secretion and synthesis of pancreatic lipase and colipase in rats. Pancreas 6: 595－602, 1991   DOI   PUBMED   ScienceOn
4 Korc M, Iwamoto Y, Sankaran H, Williams JA, Goldfine ID. Insulin action in pancreatic acini from streptozotocin-treated rats. I. Stimulation of protein synthesis. Am J Physiol 240: G56－G62, 1981b   DOI
5 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265－275, 1951   PUBMED
6 Williams JA, Goldfine ID. The insulin-pancreatic acinar axis. Diabetes 34: 980－986, 1985   DOI   ScienceOn
7 Duan RD, Erlanson-Albertsson C. Altered synthesis of some secretory proteins in pancreatic lobules isolated from streptozotocin- induced diabetic rats. Pancreas 5: 136－143, 1990   DOI   PUBMED   ScienceOn
8 Schick J, Kern H, Scheele G. Hormonal stimulation in the exocrine pancreas results in coordinate and anticoordinate regulation of protein synthesis. J Cell Biol 99: 1569－1574, 1984a   DOI   ScienceOn
9 Jo YH, Lee YL, Lee KY, Chang TM, Chey WY. Neurohormonal mechanism of pancreatic exocrine secretion stimulated by sodium oleate and L-tryptophan in dogs. Am J Physiol 263: G12－ G16, 1992
10 Okabayashi Y, Otsuki M, Ohki A, Nakamura T, Tani S, Baba S. Secretin-induced exocrine secretion in perfused pancreas isolated from diabetic rats. Diabetes 37: 1173－1180, 1988   DOI   ScienceOn
11 Soling HD, Unger KO. The role of insulin in the regulation of $\alpha$- amylase synthesis in the rat pancreas. Eur J Clin Invest 2: 199－212, 1972   DOI   ScienceOn
12 Bendayan M, Gregoire S, Immunohisto- and cytochemical studies of pancreatic enzymes in peri-insular and tele-insular acinar cells of streptozotocin-induced diabetic rats. Pancreas 12: 272－ 282, 1987
13 Saito A, Williams JA, Kanno T. Potentiation of cholecystokinininduced exocrine secretion by both exogenous and endogenous insulin in isolated and perfused rat pancreas. J Clin Invest 65: 777－782, 1980   DOI   PUBMED
14 Adler G, Beglinger C, Braum U, Reinshagen M, Koop I, Schafmayer A, Rovati L, Arnold R. Interaction of the cholinergic system and cholecystokinin in the regulation of endogenous and exogenous stimulation of pancreatic secretion in humans. Gastroenterology 100: 537－543, 1991   DOI   PUBMED
15 Park HJ, Lee YL, Kwon HY. Effects of pancreatic polypeptide on insulin action in exocrine secretion of isolated rat pancreas. J Physiol (London) 463: 421－429, 1993   DOI   PUBMED
16 Sofrankova A, Dockray GJ. Cholecystokinin- and secretin-induced pancreatic secretion in normal and diabetic rats. Am J Physiol 244: G370－G374, 1983
17 Chisholm DJ, Lazarus L, Young JD, Kraegen EW. Abnormalities of serum secretin in diabetes mellitus. Diabetes 19: 365－366, 1970
18 Li Y, Owyang C. Vagal afferent pathway mediates physiological action of cholecystokinin on pancreatic enzyme secretion. J Clin Invest 92: 418－424, 1993   DOI   ScienceOn
19 Bendayan M, Levy E. Immunohistochemical and biochemical evaluation of pancreatic lipase in acinar cells of control and streptozotocin- induced diabetic rats. Pancreas 3: 269－273, 1988   DOI   PUBMED   ScienceOn
20 Duan RD, Poensgen J, Wicker C, Westrom B, Erlanson-Albertsson C. Increase in pancreatic lipase and trypsin activity and their mRNA levels in streptozotocin- induced diabetic rats. Dig Dis Sci 34: 1243-1248, 1989   DOI   ScienceOn
21 Schick J, Verspohl R, Kern H, Scheele G. Two distinct adaptive responses in the synthesis of exocrine pancreatic enzymes to inverse changes in protein and carbohydrate in the diet. Am J Physiol 247: G611－G616, 1984b
22 Rausch U, Rudiger VK, Kern HF. In-vivo stimulation of rat pancreatic acinar cells by infusion of secretin. Cell Tissue Res 242: 641－644, 1985.   DOI   ScienceOn
23 Bazin R, Lavau M. Diet composition and insulin effect on amylase to lipase ratio in pancreas of diabetic rats. Digestion 19: 386－ 391, 1979   DOI   ScienceOn
24 Jung DH. Preparation and application of procion yellow starch for amylase assay. Clin Chim Acta 100: 7－11, 1980   DOI   ScienceOn
25 Korc M, Owerbach D, Quinto C, Rutter WL. Pancreatic islet-acinar cell interaction: amylase messenger RNA levels are determined by insulin. Science 213: 351－353, 1981a   DOI   PUBMED   ScienceOn
26 Tsai AN, Cowan MR, Johnson DG, Brannon PM. Regulation of pancreatic amylase and lipase gene expression by diet and insulin in diabetic rats. Am J Physiol 267: G575－G583, 1994
27 Wicker C, Puigserver A. Effects of inverse changes in dietary lipid and carbohydrate on the synthesis of some secretory proteins. Eur J Biochem 162: 25－30, 1987   DOI   ScienceOn
28 Liddle RA, Goldfine ID, Williams JA. Bioassay of plasma cholecystokinin in rats: effects of food, trypsin inhibitor, and alcohol. Gastroenterology 87: 542－549, 1984   PUBMED
29 Kanno T, Saito A. The potentiating influences of insulin on pancreozymin-induced hyperpolarization and amylase release in the pancreatic acinar cell. J Physiol (London) 267: 505－521, 1976
30 Otsuki M, Williams JA. Direct modulation of pancreatic CCK receptors and enzyme secretion by insulin in isolated pancreatic acini from diabetic rats. Diabetes 32: 241－246, 1983   DOI   ScienceOn
31 Lee KY, Zhou L, Ren XS, Chang TM, Chey WY. An important role of endogenous insulin on exocrine pancreatic secretion in rats. Am J Physiol 258: G268－G274, 1990
32 Lee KY, Lee YL, Kim CD, Chang TM, Chey WY. Mechanism of action of insulin on pancreatic exocrine secretion in perfused rat pancreas. Am J Physiol 267: G207－G212, 1994
33 Sabb JE, Godfrey PM, Brannon PM. Adaptive response of rat pancreatic lipase to dietary fat: effects of amount and type of fat. J Nutr 116: 892－899, 1986   DOI
34 Bonner-Weir S. The microvasculature of the pancreas, with emphasis on that of the islets of Langerhans. In: Go VLW, DiMagno ER, Gardner JD, Lebenthal E, Leber HA, Scheele GA, eds. The pancreas. 2nd ed. Raven Press, New York, p 759－768, 1993
35 Otsuki M, Williams JA. Effect of diabetes mellitus on the regulation of enzyme secretion by isolated rat pancreatic acini. J Clin Invest 70: 148－156, 1982   DOI   PUBMED
36 Duan RD, Erlanson-Albertsson C. Pancreatic lipase and colipase activity in pancreatic acinar tissue of diabetic rats. Pancreas 4: 329－334, 1989   DOI   PUBMED   ScienceOn
37 Rausch U, Rudiger K, Vasiloudes P, Kern H, Scheele G. Lipase synthesis in the rat pancreas is regulated by secretin. Pancreas 1: 522－528, 1986   DOI   PUBMED   ScienceOn
38 Chang TM, Chey WY. Radioimmunoassay of secretin: a critical review and current status. Dig Dis Sci 25: 529－552, 1980   DOI   ScienceOn
39 Mossner J, Logsdon CD, Williams JA, Goldfine ID. Insulin via its own receptor, regulates growth and amylase synthesis in pancreatic acinar AR 42 J cells. Diabetes 34: 891-897, 1985.   DOI   ScienceOn
40 Jo YH, Yoon SH, Hahn SJ, Rhie DJ, Sim SS, Kim MS. Effect of medial amygdaloid stimulation on pancreatic exocrine secretion in anesthetized rats. Pancreas 9: 117－122, 1994   DOI   PUBMED   ScienceOn