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Involvement of NMDA Receptor and L-type Calcium Channel in the Excitatory Action of Morphine  

Koo, Bon-Seop (Department of Orthopedics, Kangbuk Samsung Hospital, Sungkyunkwan University College of Medicine)
Shin, Hong-Kee (Departments of Physiology, College of Medicine, Hanyang University)
Kang, Suk-Han (Departments of Physiology, College of Medicine, Hanyang University)
Jun, Jong-Hun (Departments of Anesthesiology, College of Medicine, Hanyang University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.6, no.5, 2002 , pp. 241-246 More about this Journal
Abstract
We studied the excitatory action of morphine on the responses of dorsal horn neuron to iontophoretic application of excitatory amino acid and C-fiber stimulation by using the in vivo electrophysiological technique in the rat. In 137 of the 232 wide dynamic range (WDR) neurons tested, iontophoretic application of morphine enhanced the WDR neuron responses to N-methyl-D-aspartate (NMDA), kainate, and graded electrical stimulation of C-fibers. Morphine did not have any excitatory effects on the responses of low threshold cells. Morphine-induced excitatory effect at low ejection current was naloxone-reversible and reversed to an inhibitory action at high ejection current. NMDA receptor, calcium channel and intracellular $Ca^{2+}$ antagonists strongly antagonized the morphine-induced excitatory effect. These results suggest that changes in intracellular ionic concentration, especially $Ca^{2+},$ play an important role in the induction of excitatory effect of morphine in the rat dorsal horn neurons.
Keywords
Dorsal horn neuron; Morphine; Excitatory action; NMDA receptor; Calcium channel;
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