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Analysis of a Sphingosine 1-phosphate Receptor $hS1P_3$ in Rat Hepatoma Cells  

Im, Dong-Soon (Laboratory of Pharmacology, College of Pharmacy, Pusan National University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.6, no.3, 2002 , pp. 139-142 More about this Journal
Abstract
To examine intracellular signaling of human $S1P_3\;(hS1P_3),$ a sphingosine 1-phosphate (S1P) receptor in plasma membrane, $hS1P_3$ DNA was transfected into RH7777 rat hepatoma cell line, and the inhibition of forskolin-induced cAMP accumulation and activation of MAP kinases by S1P were tested. In $hS1P_3$ transformants, S1P inhibited forskolin-induced activation of adenylyl cyclase activity by about 80% and activated MAP kinases in dose-dependent and pertussis-toxin (PTX) sensitive manners. In oocytes expressing $hS1P_3$ receptor, S1P evoked $Cl^-$ conductance. These data suggested that PTX-sensitive G proteins are involved in $hS1P_3-mediated$ signaling, especially the positive action of S1P in cell proliferation. The potential advantages of rat hepatoma cells for the research of sphingosine 1-phosphate receptor are discussed.
Keywords
Sphingosine 1-phosphate; Lysophosphatidic acid; $S1P_3$; G Protein-coupled receptor; cAMP; MAP kinase; Proliferation;
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