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Effect of $Ca^{2+}-channel$ Blockers on Norepinephrine Release in the Rat Hippocampal Slice and Synaptosome  

Kim, Suk-Won (Department of Pharmacology, Wonkwang University School of Medicine, Wonkwang University)
Jung, Kyu-Yong (Department of Pharmacology, Wonkwang University School of Medicine, Wonkwang University)
Choi, Bong-Kyu (Department of Pharmacology, Wonkwang University School of Medicine, Wonkwang University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.6, no.2, 2002 , pp. 87-91 More about this Journal
Abstract
The aim of this study was to investigate the role of $Ca^{2+}-channel$ blockers in norepinephrine (NE) release from rat hippocampus. Slices and synaptosomes were incubated with $[^3H]-NE$ and the releases of the labelled products were evoked by 25 mM KCl stimulation. Nifedipine, diltiazem, nicardipine, flunarizine and pimozide did not affect the evoked and basal release of NE in the slice. But, diltiazem, nicardipine and flunarizine decreased the evoked NE release with a dose-related manner without any change of the basal release from synaptosomes. Also, a large dose of pimozide produced modest decrement of NE release. ${\omega}-conotoxin$ (CTx) GVIA decreased the evoked NE release in a dose-dependent manner without changing the basal release. And ${\omega}-CTxMVIIC$ decreased the evoked NE release in the synaoptosomes without any effect in the slice, but the effect of decrement was far less than that of ${\omega}-CTxGVIA.$ In interaction experiments with ${\omega}-CTxGVIA,\;{\omega}-CTxMVIIC$ slightly potentiated the effect of ${\omega}-CTxGVIA$ on NE release in the slice and synaptosomal preparations. These results suggest that the NE release in the rat hippocampus is mediated mainly by N-type $Ca^{2+}-channels,$ and that other types such as L-, T- and/or P/Q-type $Ca^{2+}-channels$ could also be participate in this process.
Keywords
Rat; Hippocampus; N-type $Ca^{2+}-channels$; Synaptosome; Norepinephrine; ${\omega}-conotoxin$;
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