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http://dx.doi.org/10.17480/psk.2015.59.5.215

Effect of Corticosterone Pretreatment on the Production of LPS-Induced Inflammatory Mediators in RAW 264.7 Cells  

Chae, Byeong Suk (College of Pharmacy, Woosuk University)
Publication Information
YAKHAK HOEJI / v.59, no.5, 2015 , pp. 215-221 More about this Journal
Abstract
Glucocorticoids are known to have anti-inflammatory effect. To investigate whether corticosterone pretreatment enhances or not lipopolysaccharide (LPS)-induced production of inflammatory mediators, RAW 264.7 cells were pretreated with various concentrations of corticosterone for 24 h and then cultured without corticosterone in the presence or absence of LPS. Our results demonstrated that LPS remarkably increased production of TNF-${\alpha}$, IL-6, IL-$1{\beta}$, vascular endothelial growth factor (VEGF), and NO (nitric oxide). Corticosterone pretreatment significantly attenuated LPS-induced production of TNF-${\alpha}$, IL-$1{\beta}$, and VEGF, while significantly enhanced IL-6 and NO. These findings suggest that corticosterone pretreatment may contribute to LPS-induced inflammatory responses in macrophages via pro- and anti-inflammatory imbalance of inflammatory mediators.
Keywords
corticosterone; TNF-${\alpha}$; IL-6; IL-$1{\beta}$; VEGF; NO; endotoxin;
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