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A Flow Cytometrical Analysis of the Antitumor and Immunostimulatory Effects of LCT-CT, a Cold-water Extract Prepared from Rice Grasshopper Oxya japonica japonica Thunberg  

Chung, Kyeong-Soo (College of Pharmacy, Chungnam National University)
Kim, Bit Na (College of Pharmacy, Chungnam National University)
Publication Information
YAKHAK HOEJI / v.58, no.3, 2014 , pp. 151-157 More about this Journal
Abstract
Water extracts of rice grasshopper (Oxya japonica japonica Thurnberg) were prepared and their antitumor and immunostimulatory activities were investigated using a flow cytometer. When LCT-CT was ip injected into ICR mice at the dose of 33.3 mg/kg before and after the implantation of $4{\times}10^5$ cells/mouse of sarcoma 180 tumor cells, it inhibited the growth of the tumor cells by 96.6%, showed lymphoblstogenic activities on the splenic lymphocytes and increased the expression of CD25 molecules on the splenic T lymphocytes. When co-cultured with the splenic lymphocytes of a BALB/c mouse, LCT-CT showed strong immunostimulatory activities at the concentration of $25{\sim}100{\mu}g/ml$ by significantly increasing lymphoblasts ratio and CD25 expression.
Keywords
rice grasshopper; Oxya japonica japonica; antitumor; sarcoma 180; CD25; flow cytometer; lymphoblast; immunostimulation;
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