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Immune-stimulating Effects of Polygonum aviculare L. Extract on Macrophages  

Jeon, Chang Bae (Department of Biology Education, College of Education, Chungbuk National University)
Kim, Young Hoon (Department of Biology Education, College of Education, Chungbuk National University)
Batsuren, Dulamjav (Institutes of Chemistry and Chemical Technology, MAS)
Tunsag, Jigjidsuren (Institutes of Chemistry and Chemical Technology, MAS)
Nho, Chu Won (Functional Food Center, Korea Institute of Science and Technology)
Pan, Cheol-Ho (Functional Food Center, Korea Institute of Science and Technology)
Lee, Jae Kwon (Department of Biology Education, College of Education, Chungbuk National University)
Publication Information
YAKHAK HOEJI / v.57, no.6, 2013 , pp. 394-399 More about this Journal
Abstract
In this study we demonstrated whether the extract of Polygonum aviculare L. (PAE) can be applied to the immune-stimulating responses in macrophages (Raw 264.7 cells). Cell viability was determined by WST-8 assay, and all four doses of PAE (5, 10, 20, and 40 ${\mu}g/ml$) had no significant cytotoxicity during the entire experimental period. PAE increased the production of inducible nitric oxide synthase (iNOS) and nitric oxide (NO), and mRNA expressions and protein levels of pro-inflammatory cytokines(tumor necrotic factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$ and IL-6) in the same cells. These immune-stimulating activities of PAE were found to be caused by the stimulation of $NF{\kappa}B$ signal and phosphorylation of MAP kinases (p38, ERK and JNK).
Keywords
Polygonum aviculare L.; macophage; cytokine; inflammation;
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