Browse > Article

Simultaneous Quantification Analysis of Multi-components on Erycibae Caulis by HPLC  

Jeon, Hye Jin (College of Pharmacy, Chung-Ang University)
Liu, Ting (College of Pharmacy, Chung-Ang University)
Whang, Wan Kyunn (College of Pharmacy, Chung-Ang University)
Publication Information
YAKHAK HOEJI / v.57, no.4, 2013 , pp. 272-281 More about this Journal
Abstract
In this study, we developed and validated the HPLC method using the isolated components from Erycibae caulis. Their structures were elucidated by spectroscopic methods including UV, $^1H$-NMR, $^{13}C$-NMR, FAB-Mass and ESI-Mass as Compound 1 (crypto-chlorogenic acid), Compound 2 (scopolin), Compound 3 (neochlorogenic acid) and Compound 4 (3,4-di-O-caffeoylquinic acid). Major three compounds and scopoletin were decided as representative components of Erycibae caulis. We established HPLC analytical method by using the representative components and 20 commercial samples which were collected considering to various cultivated area. The HPLC fingerprinting was successfully achieved with an AKZO NOBEL Kromasil 100-5C18 column. The mobile phase consisted of 0.5% acetic acid in water (A) and methanol (B) using gradient method of 85(A) to 50(A) for 35min. The fingerprints of chromatograms were recorded at an optimized wavelength of 330 nm. This developed analytical method was validated with specificity, selectivity, accuracy and precision. And it is suggested that scopolin, scopoletin, neochlorogenic acid, 3,4-di-O-caffeoylquinic acid were more than 0.162%, 0.133%, 0.057%, 0.044%, respectively. In addition, principal component analysis (PCA) was performed on the analytical data of 20 different Erycibae caulis samples in order to classify samples collected from different regions. We hope that this assay can be readily utilized as quality control method for Erycibae caulis.
Keywords
Erycibae caulis; Erycibe obtusifolia; components; Convolvulaceae; crypto-chlorogenic acid; scopolin; neochlorogenic acid; 3,4-di-O-caffeoylquinic acid; scopoletin; HPLC; PCA;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Silván, A. M., Abad, M. J., Bermejo, P. and Villar, A. : Effects of compounds extracted from Santolina oblongifolia on TXB (2) release in human platelets. Inflammopharmacol. 6, 255 (1998).   DOI
2 Li, Y., Dai, Y., Liu, M., Pan, R., Luo, Y. B., Xia, Y. F. and Xia X. F. : Scopoletin induces apoptosis of fibeoblast-like synoviocytes from adjuvant arthritis rats by a mitochondrialdependent pathway. Drug Develop. Res. 70, 378 (2009).   DOI   ScienceOn
3 Kim, H. J., Jang, S. I., Kim, Y. J., Chung, H. T., Yun, Y. G., Kang, T. H., Jeong, O. S. and Kim, Y. C. : Scopoletin suppresses proinflammatory cytokines and PGE2 from from LPS-stimulated cell line, RAW 264.7 cells. Fitoterapia. 75, 261 (2004).   DOI   ScienceOn
4 Pan, R., Dai, Y., Gao, X. H. and Xia, Y. : Scopolin isolated from Erycibe obtusifolia benth stems suppresses adjuvant-induced rat arthritis by inhibiting inflammation and angiogeneses. Int. Immunopharmacol. 9, 859 (2009).   DOI   ScienceOn
5 Liu, X. L., Zhang, L., Fu, X. L., Chen, K. and Qian, B. C. : Effects of scopoletin on PC3 cell proliferation and apoptosis. Acta. Pharmacol. Sin. 22, 929 (2001).
6 Kim, E. K., Kwon, K. B., Shin, B.C., Seo, E. A., Lee, Y. R., Kim, J. S., Park, J. W., Park, B. H. and Ryu, D. G. : Scopoletin induces apoptosis in human promyeloleukemic cells, accompanied by activations of nuclear factor BNF-Kb and caspase-3. Life Sci. 77, 824 (2005).   DOI   ScienceOn
7 식품의약품안전청 의약품평가부 : 의약품등 분석법의 밸리데이션에 대한 가이드라인. 식품의약품안전청 1 (2004)
8 Chan, E. W. C., Lim, Y. Y., Ling, S. K., Tan, S. P., Lim, K. K. and Khoo, M. G. H. : Caffeoylquinic acids from leaves of Etlingera species (Zingiberaceae). Food Science and Technology 42, 1026 (2009).
9 Nakatani, N., Kayano, S., Kikuzaki, H., Sumino, K., Katagiri, K. and Mitani, T. : Identification, quantitative determination, and antioxidative activities of chlorogenic acid isomers in prune (Prunus domestica L.). J. Agric. Food Chem. 48, 5512 (2000).   DOI   ScienceOn
10 Teh, C. H., Morita, H., Osamu, C. and Kit-Lam : 2,3-Dehydro- 4α-hydroxylongilactone, a novel quassinoid and two known phenyl propanoids from Eurycoma longifolia Jack. Food Chem. 120, 794 (2010).   DOI   ScienceOn
11 Tolonen, A., Joutsamo, T., Mattlla, S., Kamarainen, T. and Jalonen, J. : Identification of isomeric dicaffeoylquinic acids from Eleutherococcus senticosus using HPLC-ESI/TOF/MS and 1H-NMR methods. Phytochem. Anal. 13, 316 (2002).   DOI   ScienceOn
12 Basnet, P., Matsushige, K., Hase, K., Kadota, S. and Namba, T. : Four Di-O-caffeoyl quinic acid derivatives from propolis. Potent hepatoprotective activity in experimental liver injury models. Biol. Pharm. Bull. 19, 1479 (1996).   DOI   ScienceOn
13 식품의약품안전청 : 대한약전외한약(생약)규격집수재생약(KHP).
14 상해과학기술출판사 : 중화본초 제6권, 506 (1999).
15 Liu Jian : Studies on the chemical constituents and bioactivities of Erycibe Obtusfolia Benth. Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, PR China (2007.9).
16 북경학원출판사 : 중약 현대연구 및 응용(Modern study and application of material medica) 5477 (1999).
17 Silván, A. M., Abad, M. J., Bermejo, P., Sollhuber, M. and Villar, A. : Antiinflammtory activity of coumarins from Santolina oblongifolia. J. Nat. Prod. 59, 1183 (1996).   DOI   ScienceOn