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Simultaneous Determination of Liquiritin, Nodakenin, Hesperidin and Glycyrrhizin in Bojungikgi-tang Using HPLC-PDA  

Seo, Chang-Seob (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Kim, Jung-Hoon (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Publication Information
YAKHAK HOEJI / v.57, no.3, 2013 , pp. 187-193 More about this Journal
Abstract
Bojungikgi-tang has been widely used for enhancement of physical fitness in Korea. The convenient, simple, and accurate high-performance liquid chromatography (HPLC) method was established for simultaneous determination of four marker compounds, liquiritin, nodakenin, hesperidin, and glycyrrhizin in Bojungikgi-tang (Buzhongyiqi-tang in Chinese, Hochuekkito in Japanese), a traditional Korean herbal prescription. The column for optimizing HPLC separation was used a Gemini $C_{18}$ column at column oven temperature of $40^{\circ}C$ with 1.0% (v/v) aqueous acetic acid (A) and 1.0% (v/v) acetic acid in acetonitirle (B) by gradient flow. The flow rate was 1.0 ml/min and the detector was a photodiode array (PDA) set at 254 nm, 280 nm, and 335 nm. Calibration curves of four components were acquired with $r^2$ values ${\geq}0.9999$. The recoveries were found to range 92.11~105.68% with relative standard deviations (RSDs, %) value less than 2.50%. The RSD values of intraand inter-day precision were 0.07~2.50% and 0.16~1.99%, respectively. The contents of liquiritin, nodakenin, hesperidin and glycyrrhizin in Bojungikgi-tang were 3.85~3.92 mg/g, 2.27~2.32 mg/g, 4.14~4.19 mg/g, and 3.39~3.45 mg/g, respectively. The established simultaneous analysis method will be effective for quality control of Bojungikgi-tang.
Keywords
HPLC-PDA; Bojungikgi-tang; simultaneous analysis; validation;
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Times Cited By KSCI : 1  (Citation Analysis)
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