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Neuroprotective Effects of Herbal Ethanol Extract from Gynostemma pentaphyllum on Dopamine Neurons in Rotenone- and MPTP-induced Animal Model of Parkinson's Disease  

Suh, Kwang Hoon (College of Pharmacy, Chungbuk National University)
Choi, Hyun Sook (Department of Food and Nutrition, Chungcheong University)
Shin, Kun Seong (College of Pharmacy, Chungbuk National University)
Zhao, Ting Ting (College of Pharmacy, Chungbuk National University)
Kim, Seung Hwan (College of Physical Education, Kyunghee University)
Hwang, Bang Yeon (College of Pharmacy, Chungbuk National University)
Lee, Chong Kil (College of Pharmacy, Chungbuk National University)
Lee, Myung Koo (College of Pharmacy, Chungbuk National University)
Publication Information
YAKHAK HOEJI / v.57, no.2, 2013 , pp. 77-86 More about this Journal
Abstract
The neuroprotective effects of herbal ethanol extract (GP-EX) from Gynostemma pentaphyllum on dopamine neurons in animal model of Parkinson's disease (PD) were investigated. Rats and mice were administered with rotenone (2.5 mg/kg) for 28 days and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg) for 5 days for the PD models, respectively and the animals were simultaneously treated with GP-EX (30 mg/kg, daily). After preparing the PD models, the animals were also administered with L-DOPA (10 mg/kg) for 14 days with or without GP-EX treatment. Treatment with GP-EX (30 mg/kg) inhibited the rotenone- and MPTP-induced neurotoxic effects in dopamine neurons of rats or mice, which was determined by the numbers of tyrosine hydroxylase-immunohistochemical staining survival cells, as well as the levels of dopamine, 3,4-dihydroxyphenylacetic acid and homovanillic acid. GP-EX (30 mg/kg) also showed the protective effects on neurotoxicity which was induced by long-term administration of L-DOPA (10 mg/kg) in rotenone- and MPTP-induced animal model of PD. The used doses of GP-EX (30 mg/kg) did not produce any signs of toxicity, such as weight loss, diarrhea, or vomiting, in rats and mice during the treatment periods. These results suggest that GP-EX has the protective functions against chronic L-DOPA-induced neurotoxic reactions in dopamine neurons of rotenone- and MPTP-induced animal model of PD. Therefore, the natural GP-EX may be beneficial in the prevention of PD progress and L-DOPA-induced neurotoxicity in PD patients.
Keywords
Gynostemma pentaphyllum; animal model of Parkinson's disease; rotenone; MPTP; L-DOPA; tyrosine hydroxylase; dopamine neuron;
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