Browse > Article

Inhibitory Effects of Purified Bee Venom on Melanin Synthesis  

Han, Sang-Mi (Department of Agricultural Biology, National Academy and Agriculturual Science)
Kim, Jung-Min (Department of Agricultural Biology, National Academy and Agriculturual Science)
Lee, Kyung-Gill (Department of Agricultural Biology, National Academy and Agriculturual Science)
Park, Kwan-Kyu (Catholic University of Daegu School of Medicine)
Chang, Young-Chae (Catholic University of Daegu School of Medicine)
Publication Information
YAKHAK HOEJI / v.56, no.4, 2012 , pp. 254-259 More about this Journal
Abstract
To further access honeybee (Apis mellifera L.) venom (BV) as a cosmetic ingredient and potential external treatment for topical use, we investigated its ability to inhibit tyrosinase activity and melanin biosynthesis on melanogenesis in B16F1 melanoma cells. We found that BV increased the cell viability in B16F1 melanoma cell and BV (0.01~1 ${\mu}g/ml$) inhibited melanin synthesis in with 10 nM ${\alpha}$-melanocyte-stimulating hormone (${\alpha}$-MSH) for 48 h. In addition, we used reverse transcription-polymerase chain reaction and western blotting for me melanogenesis-related genes such as tyrosinase to examine the mechanisms underlying the inhibitory effects of BV on melanogensis. BV inhibited direct tyrosinase activity, which decreased melanin synthesis in ${\alpha}$-MSH stimulated B16F1 melanoma cells. Thease findings suggest that BV induces the downregulation of melanogenesis by inhibiting tyrosinase activation.
Keywords
bee venom; B16F1 melanoma cell; melanin; tyrosinase;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Urabe, K., Nakayama, J. and Hori, Y. : Mixed epidermal and dermal hypermelanoses. In "The pigmentary System: Physiology and Pathophysiology", eds. Norlund, J. J., Boissy, R. E., Hearing, V. J., King, R. A., Ortonne, J. P., Oxford University Press, New York, p. 909 (1998).
2 Chang, T. S. : An updated review of tyrosinase inhibitors. Int. J. Mol. Sci. 10, 2440 (2009).
3 Imokawa, G. : Autocrine and paracrine regulation of melanocytes in human skin and in pigmentary disorders. Pigment. Cell Res. 17, 96 (2004).
4 Ando, H., Kondoh, H., Ichihashi, M. and Hearing, V. J. : Approaches to identify inhibitors of melanin biosynthesis via the quality control of tyrosinase. J. Invest. Dermatol. 127, 751 (2007).
5 Solano, F., Briganti, S., Picardo, M. and Ghanem, G. : Hypopigmenting agents: an updated review on biological, chemical and clinical aspects. Pigment. Cell Res. 19, 550 (2006).
6 Hearing, V. J. : Biochemical control of melanogenesis and melanosomal organization. J. Investig. Dermatol. Symp. Proc. 4, 24 (1999).
7 del Marmol, V. and Beermann, F. : Tyrosinase and related proteins in mammalian pigmentation. FEBS Lett. 381, 165 (1996).
8 Mallick, S., Singh, S. K., Sarkar, C., Saha, B. and Bhadra, R. : Human placental lipid induces melanogenesis by increasing the expression of tyrosinase and its related proteins in vitro. Pigment. Cell Res. 18, 25 (2005).
9 김민정, 박상동, 이아람, 김경호, 장준혁, 김갑성 : 쥐의 Collagen 유발 관절염의 활액에서 단백분해효소의 활성 및 유리기 손상에 미치는 봉독약침의 억제효과. 대한침구학회지 19, 161 (2002).
10 Kim, H. W., Kwon, Y. B., Ham, T. W., Roh, D. H., Yoon, S. Y., Lee, H. J., Han, H. J., Yang, I. S., Beitz, A. J. and Lee, J. H. : Acupoint stimulation using bee venom attenuates formalininduced pain behavior and spinal cord fos expression in rats. J. Vet. Med. Sci. 65, 349 (2003).
11 Piek, T. : Venoms of the Hymenoptera. Academic Press, London (1986)
12 Habermann, E. and Reiz, K. G. : On the biochemistry of bee venom pep-tides, melittin and apamin. Biochemistry 343, 192 (1965).
13 Fennell, J. F., Shipman, W. H. and Cole, L. J. : Antibacterial action of a bee venom fraction (melittin) against a penicillinresistant Staphylococcus and other microorganisms. Res. Dev. Tech. Rep. 5, 1 (1967).
14 Curcio-Vonlanthen, V., Schneider, C. H., Frutig, K., Blaser, K. and Kalbacher, H. : Molecular parameters in melittin immunogenicity. J. Pept Sci. 3, 267 (1997).
15 Rudenko, S. V. and Nipot, E. E. : Modula-tion of melittininduced hemolysis of erythocytes. Biokhimiia. 61, 2116 (1996).
16 한상미, 이광길, 여주홍, 우순옥, 권해용 : 봉독의 간이 정제 방법, 대한민국특허 10-075881 (2007).
17 Han, S. M., Lee, K. G., Yeo, J. H., Kweon, H. Y., Woo, S. O., Lee, I. K., Lee, M. L., Lee, M. Y., Baek, H. J. and Bae, K. H. : Studies on the antimicorbial effect of collected bee venom using electric shock method (I). Korean J. Apiculture 20, 53 (2005).
18 Han, S. M., Lee, K. G., Yeo, J. H., Baek, H. J. and Park, K. K. : Antibacterial and anti-inflammatory effects of honeybee (Apis mellifera) venom against acne-inducing bacteria. J. Med. Plant. Res. 4, 459 (2010).
19 Han, S. M., Lee, K. G., Yeo, J. H., Kim, W. T. and Park, K. K. : Biological effects of treatment of an animal skin wound with honeybee (Apis mellifera L.) venom. J. Plast. Reconstr. Aesthet. Surg. 64, e67 (2011).
20 Chung, M. J., Walker, P. A., Brown, R. W. and Hogstrand, C. : ZINC-mediated gene expression offers protection against $H_{2}O_{2}$-induced cytotoxicity. Toxicol. Appl. Pharmacol. 205, 225 (2005).
21 Hosoi, J., Abe, E., Suda, T. and Kuroki, T. : Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 45, 1474 (1985).
22 Hodgkinson, C. A., Moore, K. J., Nakayama, A., Steingrímsson, E., Copeland, N. G., Jenkins, N. A. and Arnheiter, H. : Mutations at the mouse microphthalmia locus are associated with defects in a gene encoding a novel basic-helix-loop-helixzipper protein. Cell. 74, 395 (1993).
23 Lowings, P., Yavuzer, U. and Goding, C. R. : Positive and negative elements regulate a melanocyte-specific promoter. Mol. Cell. Biol. 12, 3653 (1992).
24 Yavuzer, U., Keenan, E., Lowings, P., Vachtenheim, J., Currie, G. and Goding, C. R. : The Microphthalmia gene product interacts with the retinoblastoma protein in vitro and is a target for deregulation of melanocyte-specific transcription. Oncogene. 10, 123 (1995).
25 Bertolotto, C. , Abbe, P., Hemesath, T. J., Bille, K., Fisher, D. E., Ortonne, J. P. and Ballotti, R. : Microphthalmia gene product as a signal transducer in cAMP-induced differentiation of melanocytes. J. Cell Biol. 142, 827 (1998).
26 Oh, M. J., Hamid, M. A., Ngadiran, S., Seo, Y. K., Sarmidi, M. R. and Park, C. S. : Ficus deltoidea (Mas cotek) extract exerted anti-melanogenic activity by preventing tyrosinase activity in vitro and by suppressing tyrosinase gene expression in B16F1 melanoma cells. Arch. Dermatol. Res. 303, 161 (2011).
27 Bertolotto, C., Buscà, R., Abbe, P., Bille, K., Aberdam, E., Ortonne, J. P. and Ballotti, R. : Different cis-acting elements are involved in the regulation of TRP1 and TRP2 promoter activities by cyclic AMP: pivotal role of M boxes (GTCATGTGCT) and of microphthalmia. Mol. Cell Biol. 18, 694 (1998).
28 Roesler, W. J., Park, E. A. and McFie, P. J. : Characterization of CCAAT/enhancer-binding protein alpha as a cyclic AMPresponsive nuclear regulator. J. Biol. Chem. 273, 14950 (1998).
29 Karin, M. : Signal transduction from the cell surface to the nucleus through the phosphorylation of transcription factors. Curr. Opin. Cell Biol. 6, 415 (1994).