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Evaluation of Oxidative Stress and Antioxidant Enzyme Expression in Human Hepatocarcinoma SK-Hep-1 Cells Treated with Stearic Acid  

Oh, Jung-Min (College of Pharmacy Chungnam National University)
Lee, Ji-Yoon (College of Pharmacy Chungnam National University)
Lee, Gwan-Ho (College of Pharmacy Chungnam National University)
Kim, Bong-Hee (College of Pharmacy Chungnam National University)
Kim, Sang-Kyum (College of Pharmacy Chungnam National University)
Publication Information
YAKHAK HOEJI / v.56, no.1, 2012 , pp. 14-19 More about this Journal
Abstract
Nonalcoholic steatohepatitis(NASH) is serious metabolic disease related to fatty acid. According to "two hit theory", fatty acid-induced oxidative stress is important factor to progress nonalcoholic steatohepatitis from steatosis. In this study, we evaluated stearic acid induced oxidative stress in human hepatocarcinoma SK-Hep-1 cell. Cell viability, reactive oxygen species (ROS) production, glutathione (GSH), malondialdehyde and expression of antioxidant enzymes were determined at various time-points and concentrations of stearic acid. At 0.2 mM, non-toxic concentration, of stearic acid, production of ROS was significantly increased at 24 hours and the level of GSH was significantly decreased. Expression of superoxide dismutase-1 and 2 was slightly increased in 0.2 mM stearic acid at 24 hours. These results represent that the non-toxic concentration of stearic acid resulted in oxidative stress, suggesting that stearic acid may play a critical role in development of steatohepatitis.
Keywords
NASH; oxidative stress; stearic acid; steatosis; saturated fatty acid;
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