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Effect of Bee Venom on Glutamate-mediated Excitotoxicity in NSC-34 Motor Neuronal Cells  

Lee, Sang-Min (Department of Standard Research, Korea Institute of Oriental Medicine)
Choi, Sun-Mi (Department of Standard Research, Korea Institute of Oriental Medicine)
Jung, So-Young (Department of Standard Research, Korea Institute of Oriental Medicine)
Yang, Eun-Jin (Department of Standard Research, Korea Institute of Oriental Medicine)
Publication Information
YAKHAK HOEJI / v.55, no.5, 2011 , pp. 385-390 More about this Journal
Abstract
Bee venom (BV), which is extracted from honeybees, has been used in traditional Korean medical therapy. Glutamate-mediated excitotoxicity contributes to neuronal death in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) or Alzheimer's disease (AD). This study is to investigate the effect of BV on glutamate-induced neurotoxicity on NSC-34 motor neuron cells. To determine the viability of motor neuronal cells, we performed with MTT assays in glutamate-treated NSC-34 cell with BV or without. For the measurement of oxidative stress, DCF assay was used in glutamate-treated NSC-34 motor neuronal cells with BV or without. To investigate the molecular mechanism of BV against glutamate-mediated neurotoxicity in NSC-34 cells, western blot analysis was used. Glutamate significantly decreased cell viability by glutamate dose- or treatment time-dependent manner in NSC-34 cells. However, BV pre-treatment dramatically inhibited glutamate-induced neuronal cell death. Furthermore, we found that BV increased the expression of Bcl-2 protein that is anti-apoptotic protein and reduced the generation of oxidative stress. BV has a neuroprotective role against glutamate neurotoxicity by an increase of anti-apoptotic protein. It suggests that BV may be useful for the reduction of neuronal cell death in neuronal disease models.
Keywords
bee venom; glutamate; motor neuronal cell; oxidative stress;
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