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Effects of Chrysosplenol C on Intracellular $Ca^{2+}$ Transient in Isolated Rat Ventricular Myocytes  

Jung, Suk-Han (College of Pharmacy, IDRD, Chungnam National University)
Huong, Do Thi Thu (Institute of Chemistry, Vietnam Academy of Science and Technology (VAST))
Sung, Tran Van (Institute of Chemistry, Vietnam Academy of Science and Technology (VAST))
Cuong, Nguyen Manh (Department of Bioactive Products, Institute of Chemistry, VAST)
Kim, Young-Ho (College of Pharmacy, IDRD, Chungnam National University)
Woo, Sun-Hee (College of Pharmacy, IDRD, Chungnam National University)
Publication Information
YAKHAK HOEJI / v.55, no.2, 2011 , pp. 168-171 More about this Journal
Abstract
Chrysosplenol C [5,6-dihydroxy-2-(4-hydroxy-3-methoxyphenyl)-3,7-dimethoxychromen-4-one] is a flavonoid found in Miliusa balansae and Pterocaulon sphacelatum. We have recently shown that chrysosplenol C has positive inotropic effect in isolated rat ventricular myocytes. In the present study, we explored a possible mechanism for the positive inotropic effect of chrysosplenol C by examining intracellular $Ca^{2+}$ transients during action potentials. The intracellular $Ca^{2+}$ transients were measured by confocal $Ca^{2+}$ imaging in field-stimulated single rat ventricular myocytes. Chrysosplenol C (50 ${\mu}M$) significantly increased the magnitudes (${\Delta}F/F_0$) of $Ca^{2+}$ transients (control, $1.08{\pm}0.05$; chrysosplenol C, $1.25{\pm}0.03$; n=8, P<0.01). Half decay time of the action potential-induced $Ca^{2+}$ transient was not altered by chrysosplenol C (50 ${\mu}M$) (control, $154{\pm}6$ ms; chrysosplenol C, $167{\pm}11$ ms; n=21). The $Ca^{2+}$ content in the sarcoplasmic reticulum (SR), measured as caffeine (10 mM)-induced $Ca^{2+}$ transient, was significantly decreased by chrysosplenol C (50 ${\mu}M$). These results indicate that chrysosplenol C increases $Ca^{2+}$ transients without altering $Ca^{2+}$ removal kinetics in ventricular myocytes, providing a possible mechanism for its positive inotropic effect.
Keywords
chrysosplenol C; intercellular $Ca^{2+}$ transient; ventricular myocytes; SR $Ca^{2+}$ content; $Ca^{2+}$ transient decay;
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