Browse > Article

Immunoadjuvant Activity of Chlorogenic Acid  

Han, Yong-Moon (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University)
Publication Information
YAKHAK HOEJI / v.54, no.6, 2010 , pp. 494-499 More about this Journal
Abstract
We have been focussing on discovery of natural compounds that have immunoregulatory activities for many years. In the present study, we investigated if chlorogenic acid (CRA), a polyphenolic compound, has an immunoadjuvant activity. Prior to examining the immunoadjuvant activity, effect of CRA on proliferation of T- or B-lymphocyte was determined. Results showed that CRA enhanced the proliferation of those lymphocytes in dose-dependant manner (P<0.05), and the proliferation enhancement by CRA was appeared to be more effective to B-cells than to T-cells. Based on these observations, it was tested with bovine serum albumin (BSA) and Candida albicans cell wall (CACW) as antigenic sources if CRA has an immunoadjuvant activity. In experiments, BSA alone or a mixture of BSA plus CRA was injected intraperitoneally to mice (BALB/c strain). For a negative control, mice were given only diluent (DPBS) by the same route. In other experiment, CACW was tested by the same way as did with BSA. Three weeks after the first immunization these animals were boosted. Antisera collected from the mice one week after the booster were analyzed by ELISA. Results displayed that the induction of anti-BSA antibody was increased in mice that received the mixture of BSA and CRA as compared to anti-BSA induction in BSA only-given mice groups (P<0.05). In case of CACW, a similar observation as did with BSA was made, resulting in that there was app. 40% increased production of the anti-CACW antiserum from the combination (CACW plus CRA)-received mice as compared to antiserum induction from CACW alone-given animals. Taken all together, these data indicate that CRA has an ability of enhancing antibody production regardless of nature of antigenic sources. Presumably, activation of B-cell proliferation by CRA may plays an important role in the immunoadjuvant activity of the polyphenolic compound.
Keywords
chlorogenic acid; immunoadjuvant; T-lymphocyte; B-lymphocyte; BSA; CACW;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Han, Y. : $18{\beta}$-glycyrrhetinic acid induces protective anti- Candida albicans antibody by its immunoadjuvant activity. Yakhak Hoeji 52, 494 (2008).   과학기술학회마을
2 Ghochikyan, A., Mkrtichyan, M., Loukinov, D., Mamikonyan, G., Pack, S. D., Movsesyan, N., Ichim, T. E., Cribbs, D. H., Lobanenkov, V. V. and Agadjanyan, M. G. : Elicitation of T cell responses to histologically unrelated tumors by immunization with the novel cancer-testis antigen, brother of the regulator of imprinted sites. J. Immunol. 178, 566 (2007).   DOI
3 Han, Y., Morrison, R. P. and Cutler, J. E. : A vaccine and monoclonal antibodies that enhance mouse resistance to Candida albicans vaginal infection. Infect. Immun. 66, 5771 (1998).
4 Han, Y., Kanbe, T. K., Cherniak, R. and Cutler, J. E. : Biochemical characterization of Candida albicans epitopes that can elicit protective and nonprotective antibodies. Infect. Immun. 65, 4100 (1997).
5 Han, Y. and Cutler, J. E. : Antibody response that protects against disseminated candidiasis. Infect. Immun. 63, 2714 (1995).
6 Han, Y., van Rooijen, N. and Cutler, J. E. : Binding of Candida albicans yeast cells to mouse politeal lymph node is mediated by macrophages. Infect. Immun. 61, 3244 (1993).
7 Han, Y., Ulrich, M. A. and Cutler, J. E. : Candida albicans mannan extract-protein conjugates induce a protective immune response against experimental candidiasis. J. Infect. Dis. 179, 1477 (1999).   DOI   ScienceOn
8 Han, Y., Riesselman, M. H. and Cutler, J. E. : Protection against candidiasis by an immunoglobulin G3 (IgG3) monoclonal antibody specific for the same mannotriose as an IgM protective antibody. Infect. Immun. 68, 1649 (2000).   DOI   ScienceOn
9 Corthay, A. : A three-cell model for activation of naive T helper cells. Scand J. Immunol. 64, 93 (2006).   DOI   ScienceOn
10 Han, Y., Kozel, T. R., Zhang, M. X., MacGill, R. S., Carroll, M. C. and Cutler, J. E. : Complement is essential for protection by an IgM and an IgG3 monoclonal antibody against experimental, hematogenously disseminated candidiasis. J. Immunol. 167, 1550 (2001).   DOI
11 Dos Santos, M. D., Almeida, M. C., Lopes, N. P. and De Souza, G. E. P. : Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid. Biol. Pharm. Bull. 29, 2236 (2006).   DOI   ScienceOn
12 Ma, C., Kully, M., Khan, J. K., Hattori, M. and Daneshtalab, M. : Synthesis of chlorogenic acid derivatives with promising antifungal activity. Bioorganic. Med. Chem. 15, 6830 (2007).   DOI   ScienceOn
13 Lee, J. H., Park, J. H., Kim, Y. S. and Han, Y. : Chlorogenic acid, a polyphenolic compound, treats mice with septic arthritis caused by Candida albicans. Int. Immunopharmacol. 8, 1681 (2008).   DOI   ScienceOn
14 Lee, J. H. and Han, Y. : Ginsenoside Rg1 helps mice resist to disseminated candidiasis by Th1 type differentiation of CD4+ T cell. Int. Immunopharm. 6, 1424 (2006).   DOI   ScienceOn
15 Kensil, C. R., Patel, U., Lennick, M. and Marciani, D. : Separation and characterization of saponins with adjuvant activity from Quillaja saponaria Molina cortex. J. Immunol. 146, 431 (1991).
16 White, K., Rades, T., Kearns, P., Toth, I. and Hook, S. : Immunogenicity of liposomes containing lipid core peptides and the adjuvant Quil A. Pharm. Res. 23, 1473 (2006).   DOI   ScienceOn
17 Lee, J. H., Park, J. H., Kim, Y. S. and Han, Y. : Chlorogenic acid, a polyphenolic compound, treats mice with septic arthritis caused by Candida albicans. Int. Immunopharmacol. 8, 1681 (2008).   DOI   ScienceOn
18 Lee, J. H., Lee, J. Y., Park, J. H., Jung, H. S., Kim, J. S., Kang, S. S., Kim, Y. S. and Han, Y. : Immunoregulatory activity by daucosterol, a beta-sitosterol glycoside, induces protective Th1 immune response against disseminated Candidiasis in mice. Vaccine. 25, 3834 (2007).   DOI   ScienceOn
19 Han, Y. : Effect of $18{\beta}$-glycyrrhetinic acid on septic arthritis caused by Candida albicans. Yakhak Hoeji 51, 325 (2007).   과학기술학회마을
20 MacLeod, M. K., McKee, A., Crawford, F., White, J., Kappler. J. and Marrack, P. : CD4 memory T cells divide poorly in response to antigen because of their cytokine profile. Proc. Natl. Acad. Sci. (USA) 23, 14521 (2008).
21 Chen, G. H., McNamara, D. A., Hernandez, Y., Huffnagle, G. B., Toews, G. B. and Olszewski, M. A. : Inheritance of immune polarization patterns is linked to resistance versus susceptibility to Cryptococcus neoformans in a mouse model. Infect. Immun. 76, 2379 (2008).   DOI   ScienceOn
22 Weaver, C. T., Hatton, R. D., Mangan, P. R. and Harrington, L. E. : IL-17 family cytokines and the expanding diversity of effector T cell lineages. Annu. Rev. Immunol. 25, 821 (2007).   DOI   ScienceOn