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Structural Analysis of Oligosaccharides of a Plant Glycoprotein  

Bae, Jae-Woo (College of Pharmacy, Chung-Ang University)
Park, Byung-Tae (College of Pharmacy, Chung-Ang University)
Yoon, Doo-Chun (College of Pharmacy, Chung-Ang University)
Kim, Joo-Young (College of Pharmacy, Chung-Ang University)
Hwang, Hye-Sung (College of Pharmacy, Chung-Ang University)
Park, Hyun-Joo (College of Pharmacy, Chung-Ang University)
Na, Jong-Chun (College of Pharmacy, Chung-Ang University)
Kim, Ha-Hyung (College of Pharmacy, Chung-Ang University)
Publication Information
YAKHAK HOEJI / v.54, no.6, 2010 , pp. 449-454 More about this Journal
Abstract
The glycosylation of glycoproteins from mammalian or plants can affect their efficacy, stability, solubility, and half-life. In the present study, we investigated plant glycosylation and their relative intensity (%) in a plant carbohydratebinding protein with the hemagglutination and antiproliferative activities. The hemagglutination activity on the deglycosylated protein was decreased as a 16-fold than that of intact glycoprotein. Using the HPLC with fluorescence detector and mass spectrometer, the major eight bi- or triantennary oligosaccharides containing xylose, fucose, mannose, galactose, and N-acetylglucosamine were identified and structurally characterized. The present results indicate that the oligosaccharides on this plant glycoprotein is necessary for their own property.
Keywords
plant glycoprotein; oligosaccharide; structure;
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