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Protective Effect of the 70% Ethanolic Extract of Alpinia officinarum and Galangin Against $KBrO_3$-induced DNA and Chromosomal Damage in Mice  

Yang, Hye-Eun (College of Pharmacy, Kangwon National University)
Heo, Moon-Young (College of Pharmacy, Kangwon National University)
Publication Information
YAKHAK HOEJI / v.54, no.6, 2010 , pp. 423-428 More about this Journal
Abstract
The aim of this study was to evaluate the in vivo effect of galangin and the 70% ethanolic extract of Alpinia officinarum (AO) toward $KBrO_3$-induced DNA and chromosomal damage in mice. Galangin and AO inhibited the formation of 8-hydroxy-2'-deoxyguanosine (8-OH2'dG) as an indicator of DNA oxidative damage in the liver cell. Galangin and AO showed the inhibitory effect on the formation of DNA single strand break in the splenocyte by single cell gel electrophoresis (SCGE) assay and also inhibited micronucleated reticulocyte (MNRET) formation of peripheral blood in tail blood of mice. Vit-E revealed antigenotoxic effects in DNA and chromosome levels, but galangin was more potent active compound compare to vit-E under our experimental conditions. The results suggest that the extract of Alpinia officinarum containing galangin can modify the oxidative DNA and chromosomal damage and may act as chemopreventive agent against oxidative stress in vivo.
Keywords
galangin; vit-E; Alpinia officinarum; potassium bromate; 8-hydroxy-2'-deoxyguanosine; oxidative DNA and chromosomal damage; antigenotoxic effect;
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Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 김수희, 허문영 : $H_2O_2$ 유도 8-OH2'dG 생성 및 DNA single strand break에 미치는 Galangin의 억제효과. 약학회지 54, 32 (2010).   과학기술학회마을
2 Matsuda, H., Ando, S., Kato, T., Morikawa, T. and Yoshikawa, M. : Inhibitors from the rhizomes of Alpinia officinarum on production of nitric oxide in lipopolysaccharide-activated macrophages and the structural requirements of diarylheptanoids for the activity. Bioorg Med. Chem. 14(1), 138 (2006).   DOI   ScienceOn
3 Mosesso, P., Penna, S., Pepe, G., Lorenti-Garcia, C. and Palitti, F. : Potassium bromate but not X-rays cause unexpectedly elevated levels of DNA breakage similar to those induced by ultraviolet light in Cockayne syndrome (CS-B) fibroblasts. Cytogenet. Genome Res. 104(1-4), 78 (2004).
4 Parsons, J. L. and Chipman, J. K. : The role of glutathione in DNA damage by potassium bromate in vitro. Mutagenesis 15(4), 311 (2000).   DOI   ScienceOn
5 Sai, K., Takagi, A., Umemura, T., Hasegawa, R. and Kurokawa, Y. : Relation of 8-hydroxydeoxyguanosine formation in rat kidney to lipid peroxidation, glutathione level and relative organ weight after a single administration of potassium bromate. Jpn. J. Cancer Res. 82(2), 165 (1991).   DOI
6 Awogi, T., Murata, K., Uejima, M., Kuwahara, T., Asanami, S., Shimono, K. and Morita, T. : Induction of micronucleated reticulocytes by potassium bromate and potassium chromate in CD-1 male mice. Mutat. Res. 278(2-3), 181 (1992).   DOI   ScienceOn
7 Sai, K., Hayashi, M., Takagi, A., Hasegawa, R., Sofuni, T. and Kurokawa, Y. : Effects of antioxidants on induction of micronuclei in rat peripheral blood reticulocytes by potassium bromate. Mutat. Res. 269(1), 113 (1992).   DOI   ScienceOn
8 Kurokawa, Y., Matsushima, Y., Takamura, N., Imazawa, T. and Hayashi, Y. : Relationship between the duration of treatment and the incidence of renal cell tumors in male F344 rats administered potassium bromate. Jpn. J. Cancer Res. 78(4), 358 (1987).
9 이승철, 허찬, 이승현, 김현표, 허문영 : 야채 및 과일추출물의 항산화작용과 산화적 염색체손상에 대한 억제효과. 약학회지 48(2), 111 (2004).   과학기술학회마을
10 Sai, K., Hayashi, M., Takagi, A., Hasegawa, R., Sofuni, T. and Kurokawa, Y. Effects of antioxidants on induction of micronuclei in rat peripheral blood reticulocytes by potassium bromate. Mutat. Res. 269(1), 113 (1992).   DOI   ScienceOn
11 Sai, K., Tyson, C. A., Thomas, D. W., Dabbs, J. E., Hasegawa, R. and Kurokawa, Y. : Oxidative DNA damage induced by potassium bromate in isolated rat renal proximal tubules and renal nuclei. Cancer Lett. 87(1), 1 (1994).   DOI   ScienceOn
12 Meyer, J. J., Afolayan, A. J., Taylor, M. B. and Erasmus, D. : Antiviral activity of galangin isolated from the aerial parts of Helichrysum aureonitens. J. Ethnopharmacol. 56(2), 165 (1997).   DOI   ScienceOn
13 Cholbi, M. R., Paya, M. and Alcaraz, M. J. : Inhibitory effects of phenolic compounds on CCl4-induced microsomal lipid peroxidation. Experientia 47(2), 195 (1991).   DOI   ScienceOn
14 De Souza, R. F. and De Giovani, W. F. : Antioxidant properties of complexes of flavonoids with metal ions. Redox Rep. 9(2), 97 (2004).   DOI   ScienceOn
15 Rapta, P., Misik, V., Stasko A. and Vrabel I. : Redox intermediates of flavonoids and caffeic acid esters from propolis: an EPR spectroscopy and cyclic voltammetry study. Free Radic Biol. Med. 18(5), 901 (1995).   DOI   ScienceOn
16 Myara, I., Pico, I., Vedie, B. and Moatti, N. : A method to screen for the antioxidant effect of compounds on low-density lipoprotein(LDL) : illustration with flavonoids. J. Pharmacol. Toxicol. Methods 30(2), 69 (1993).   DOI   ScienceOn
17 Hayashi, M., Morita, T., Kodama, T., Sofuni, T. and Ishidate, Jr. M. : The micronucleus assay with peripheral blood reticulocytes using acridine orange-coated slides. Mutation Res. 278, 209 (1990).
18 Shigenaga, M. K., Park, J. W., Cundy, K. C., Gimeno, C. J. and Ames, B. N. : In vivo oxidative DNA damage : Measurement of 8-hydroxy-2'-deoxyguanosine in DNA and urine by highperformance liquid chromatography with electrochemical detection. Methods Enzymol. 186, 521 (1990).   DOI
19 Singh, N. P., McCoy, M. T., Tice, R. R. and Schneider, E. L. : A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research 175, 184   DOI   ScienceOn
20 Olive, P. L., Banath, R. E. and Durand, R. E. : Heterogenecity in radiation-induced DNA damage and repair in tumor and normal cells measured using the comet assay. Radiat. Res. 122, 86 (1990).   DOI   ScienceOn
21 Afolayan, A. J. and Meyer, J. J. : The antimicrobial activity of 3,5,7- tri-hydroxyflavone isolated from the shoots of Helichrysu aureonitens. J. Ethnopharmacol. 57(3), 177 (1997).   DOI   ScienceOn
22 Ames, B. N. : Endogeneous DNA damage as related to cancer and aging. Mutation Res. 214, 41 (1989).   DOI   ScienceOn
23 Umemura, T., Takagi, A., Sai, K., Hasegawa, R. and Kurokawa, Y. : Oxidative DNA damage and cell proliferation in kidneys of male and female rats during 13-weeks exposure to potassium bromate ($KBrO_3$). Arch. Toxicol. 72(5), 264 (1988).
24 Ames, B. N. : Dietary carcinogens and anticarcinogens. Oxygen radical and degenerative diseases. Science 221, 1256 (1983).   DOI   ScienceOn
25 Lee, S. C., Shin, K. S. and Heo, M. Y. : Protection of ROS induced cytotoxicity and DNA damage by the extract of Alpinia officinarum. J. Food Hygiene and Safety 17, 106 (2002).   과학기술학회마을
26 Park, J. W., Cundy, K. C. and Ames, B. N. : Detection of DNA adducts high-performance liquid chromatography with electrochemical detection. Carcinogenesis 10, 827 (1989).   DOI   ScienceOn
27 소학관편 : 중약대사전 제2권, 상해과학기술출판사, 상해 p 782 (1985).
28 Hiramoto, K., Ojima, N., Sako, K. and Kikugawa, K. : Effect of plant phenolics on the formation of the spin-adduct of hydroxyl radical and the DNA strand breaking by hydroxyl radical. Biol. Pharm. Bull. 19, 558 (1996).   DOI   ScienceOn
29 Noda, Y., Anzai, K., Mori, A., Kohno, M., Shinme, M. and Packer, L. : Hydroxyl and superoxide anion radical scavenging activities of natural source antioxidants using the computerized JES-FR30 ESR spectrometer system. Biochem. Mol. Biol. Int. 42(1), 35 (1997).
30 Afanas'ev, I. B. and Polozova, N. I. : One electron oxidation of p- and o-dihydroxy benzenes by oxygen radical anion in aprotic medium. Zh. Organ. Khim. 26, 1013 (1978).