Browse > Article

The Negative Role of PDE4 on PTH-induced Signaling in Osteoblasts  

Park, Hyo-Jung (College of Pharmacy, Sookmyung Women's University)
Noh, A-Long-Sae-Mi (College of Pharmacy, Sookmyung Women's University)
Lee, Jung-Min (College of Pharmacy, Sookmyung Women's University)
Yim, Mi-Jung (College of Pharmacy, Sookmyung Women's University)
Publication Information
YAKHAK HOEJI / v.54, no.5, 2010 , pp. 410-415 More about this Journal
Abstract
We explored the role of phosphodiesterase 4 (PDE4) on parathyroid (PTH)-induced signaling in osteoblasts. PTH was shown to increase the activity of PDE, mainly PDE4, in osteoblasts, which is partly attributable to elevated PDE4B and PDE4D mRNA expression. The use of PDE4 inhibitor strengthened the PTH-induced extracellular signal-regulated kinase (ERK) and p38 MAP kinase (MAPK) activation. Furthermore, the PDE4 inhibitor stimulated PTH-induced receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL) expression in osteoblasts, which in turn increased osteoclast formation. Taken together, these data suggest the negative role of PDE4 on PTH-induced signaling in osteoblasts.
Keywords
PTH; PDE4; osteoblast; osteoclast;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Xin, W., Tran, T. M., Richter, W., Clark, R. B. and Rich, T. C. : Roles of GRK and PDE4 activities in the regulation of beta2 adrenergic signaling. J. Gen. Physiol. 131, 349 (2008).   DOI   ScienceOn
2 Wakabayashi, S., Tsutsumimoto, T., Kawasaki, S., Kinoshita, T., Horiuchi, H. and Takaoka, K. : Involvement of phosphodiesterase isozymes in osteoblastic differentiation. J. Bone. Miner. Res. 17, 249 (2002).   DOI   ScienceOn
3 Takami, M., Cho, E. S., Lee, S. Y., Kamijo, R. and Yim, M. : Phosphodiesterase inhibitors stimulate osteoclast formation via TRANCE/RANKL expression in osteoblasts: possible involvement of ERK and p38 MAPK pathways. FEBS Lett. 31, 832 (2005).
4 Lacey, D. L., Timms, E., Tan, H. L., Kelley, M. J., Dunstan, C. R., Burgess, T., Elliott, R., Colombero, A., Elliott, G., Scully, S., Hsu, H., Sullivan, J., Hawkins, N., Davy, E., Capparelli, C., Eli, A., Qian, Y. X., Kaufman, S., Sarosi, I., Shalhoub, V., Senaldi, G., Guo, J., Delaney, J. and Boyle, W. J. : Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell 93, 165 (1998).   DOI   ScienceOn
5 Bruss, M. D., Richter, W., Horner, K., Jin, S. L. and Conti, M. : Critical role of PDE4D in beta2-adrenoceptor-dependent cAMP signaling in mouse embryonic fibroblasts. J. Biol. Chem. 283, 22430 (2008).   DOI   ScienceOn
6 Kondo, H., Guo, J. and Bringhurst, F. R. : Cyclic adenosine monophosphate/protein kinase A mediates parathyroid hormone/parathyroid hormone-related protein receptor regulation of osteoclastogenesis and expression of RANKL and osteoprotegerin mRNAs by marrow stromal cells. J. Bone. Miner. Res. 17, 1667 (2002).   DOI   ScienceOn
7 Wong, B. R., Rho, J., Arron, J., Robinson, E., Orlinick, J., Chao, M., Kalachikov, S., Cayani, E., Bartlett, F. S. 3rd, Frankel, W. N., Lee, S. Y. and Choi, Y. : TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun Nterminal kinase in T cells. J. Biol. Chem. 272, 25190 (1997).   DOI   ScienceOn
8 Datta, N. S. and Abou-Samra A. B. : PTH and PTHrP signaling in osteoblasts. Celllular Signalling 21, 1245 (2009).   DOI   ScienceOn
9 Conti, M. and Beavo, J. : Biochemistry and physiology of cyclic nucleotide phosphodiesterases: Essential components in cyclic nucleotide signaling. Annu. Rev. Biochem. 76, 481 (2007).   DOI   ScienceOn
10 Essayan, D. M. : Cyclic nucleotide phosphodiesterases. J. Allergy Clin. Immunol. 108, 671 (2001).   DOI   ScienceOn
11 Suda, T., Takahashi, N., Udagawa, N., Jimi, E., Gillespie, M. T. and Martin, T. J. : Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr. Rev. 20, 345 (1999).   DOI   ScienceOn
12 Yasuda, H., Shima, N., Nakagawa, N., Yamaguchi, K., Kinosaki, M., Mochizuki, S., Tomoyasu, A., Yano, K., Goto, M., Murakami, A., Tsuda, E., Morinaga, T., Higashio, K., Udagawa, N., Takahashi, N. and Suda, T. : Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesisinhibitory factor and is identical to TRANCE/RANKL. Proc. Natl. Acad. Sci. USA 95, 3597 (1998).   DOI   ScienceOn
13 Takahashi, N., Akatsu, T., Udagawa, N., Sasaki, T., Yamaguchi, A., Moseley, J. M., Martin, T. J. and Suda, T. : Osteoblastic cells are involved in osteoclast formation. Endocrinology 123, 2600 (1988).   DOI   ScienceOn