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Gardenia jasminoides Prevents Galactosamine-Induced Acute Hepatitis in Rats  

Kim, Hyo-Yeon (School of Pharmacy, Sungkyunkwan University)
Koh, Eun-Ji (School of Pharmacy, Sungkyunkwan University)
Park, Ju-Hyun (School of Pharmacy, Sungkyunkwan University)
Lee, Sun-Mee (School of Pharmacy, Sungkyunkwan University)
Publication Information
YAKHAK HOEJI / v.54, no.5, 2010 , pp. 403-409 More about this Journal
Abstract
Gardenia jasminoides is a popular traditional herb used to treat inflammatory diseases including liver disorders. This study was performed to examine protective effect of G. jasminoides on galactosamine (GalN)-induced acute hepatitis. Rats were treated intraperitoneally with GalN (700 mg/kg). G. jasminoides (30, 100 and 300 mg/kg) was administered orally 48, 24, and 2 h before and 6 h after GalN injection. Serum ALT and AST activities were significantly increased after GalN injection, and these increases were attenuated by G. jasminoides. Histological studies showed that G. jasminoides inhibited hepatocellular necrosis with inflammatory cell infiltration. GalN decreased the serum levels of total cholesterol and this decrease was attenuated by G. jasminoides. Hepatic glutathione content was decreased and lipid peroxidation was increased after GalN treatment and these changes were attenuated by G. jasminoides. Furthermore, the level of tumor necrosis factor-${\alpha}$ mRNA expression was significantly increased after GalN injection, and this increase was attenuated by G. jasminoides. The level of interleukin-10 mRNA expression was significantly increased after GalN injection, and this increase was augmented by G. jasminoides. Our results suggest that G. jasminoides ameliorates GalN-induced acute hepatitis and this protection is likely due to antioxidative activity and regulation of inflammatory mediators.
Keywords
galactosamine; Gardenia jasminoides; hepatitis; inflammation; oxidative stress;
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1 Suresh, V. and Asha, V. V. : Preventive effect of ethanol extract of Phyllanthus rheedii Wight. on D-galactosamine induced hepatic damage in Wistar rats. J. Ethnopharmacol. 116, 447 (2008).   DOI   ScienceOn
2 Blackwell, T. S. and Christman, J. W. : Sepsis and cytokines: current status. Br. J. Anaesth. 77, 110 (1996).   DOI   ScienceOn
3 Serracino-Inglott, F., Habib, N. A. and Mathie, R. T. : Hepatic ischemia-reperfusion injury. Am. J. Surg. 181, 160 (2001).   DOI   ScienceOn
4 Abe, K., Ijiri, M., Suzuki, T., Taguchi, K., Koyama, Y. and Isemura, M. : Green tea with a high catechin content suppresses inflammatory cytokine expression in the galactosamine-injured rat liver. Biomed. Res. 26, 187 (2005).   DOI   ScienceOn
5 Wang, G. X., Ben, C. E. and Ye, B. K. : Reparative effects of biphenyl dimethyl dicarboxylate on experimental liver injury in rats with histochemical and electronmicroscopy study. Zhong Xi Yi Jie He Za Zhi. 8, 158 (1988).
6 Akao, T., Kobashi, K. and Aburada, M. : Enzymic studies on the animal and intestinal bacterial metabolism of geniposide. Biol. Pharm. Bull. 17, 1573 (1994).   DOI   ScienceOn
7 Kawata, Y., Hattori, M., Akao, T., Kobashi, K. and Namba, T. : Formation of nitrogen-containing metabolites from geniposide and gardenoside by human intestinal bacteria. Planta Med. 57, 536 (1991).   DOI   ScienceOn
8 Decker, K. and Keppler, D. : Galactosamine hepatitis: key role of the nucleotide deficiency period in the pathogenesis of cell injury and cell death. Rev. Physiol. Biochem. Pharmacol. 71, 77 (1974).   DOI
9 Jaishree, V. and Badami, S. : Antioxidant and hepatoprotective effect of swertiamarin from Enicostemma axillare against dgalactosamine induced acute liver damage in rats. J. Ethnopharmacol. 130, 103 (2010).   DOI   ScienceOn
10 Pushpavalli, G., Kalaiarasi, P., Veeramani, C. and Pugalendi, K. V. : Effect of chrysin on hepatoprotective and antioxidant status in D-galactosamine-induced hepatitis in rats. Eur. J. Pharmacol. 631, 36 (2010).   DOI   ScienceOn
11 Lin, B. R., Yu, C. J., Chen, W. C., Lee, H. S., Chang, H. M., Lee, Y. C., Chien, C. T. and Chen, C. F. : Green tea extract supplement reduces D-galactosamine-induced acute liver injury by inhibition of apoptotic and proinflammatory signaling. J. Biomed. Sci. 16, 35 (2009).   DOI   ScienceOn
12 Loguercio, C. and Federico, A. : Oxidative stress in viral and alcoholic hepatitis. Free Radic. Biol. Med. 34, 1 (2003).   DOI   ScienceOn
13 Shin, J. K., Kim, H. Y. and Lee, S. M. : Protective effect of Gardenia jasminoides against carbon tetrachloride-induced acute hepatotoxicity. J. Pharm. Soc. Korea 54, 55 (2010).   과학기술학회마을   DOI
14 Jaeschke, H., Smith, C. V. and Mitchell, J. R. : Reactive oxygen species during ischemia-reflow injury in isolated perfused rat liver. J. Clin. Invest. 81, 1240 (1988).   DOI
15 Bremer, C., Bradford, B. U., Hunt, K. J., Knecht, K. T., Connor, H. D., Mason, R. P. and Thurman, R. G. : Role of Kupffer cells in the pathogenesis of hepatic reperfusion injury. Am. J. Physiol. 267, G630 (1994).
16 Keppler, D., Lesch, R., Reutter, W. and Decker, K. : Experimental hepatitis induced by D-galactosamine. Exp. Mol. Pathol. 9, 279 (1968).   DOI   ScienceOn
17 Farber, J. L., Gill, G. and Konishi, Y. : Prevention of galactosamine-induced liver cell necrosis by uridine. Am. J. Pathol. 72, 53 (1973).
18 Ohkawa, H., Ohishi, N. and Yagi, K. : Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351 (1979).   DOI   ScienceOn
19 Opal, S. M. and DePalo, V. A. : Anti-inflammatory cytokines. Chest 117, 1162 (2000).   DOI   ScienceOn
20 Coen, M., Want, E. J., Clayton, T. A., Rhode, C. M., Hong, Y. S., Keun, H. C., Cantor, G. H., Metz, A. L., Robertson, D. G., Reily, M. D., Holmes, E., Lindon, J. C. and Nicholson, K. J. : Mechanistic aspects and novel biomarkers of responder and non-responder phenotypse in galactosamine-induced hepatitis. J. Proteome. Res. 8, 5175 (2009).   DOI   ScienceOn
21 Prokopenko, V. M., Partsalis, G. K., Pavlova, N. G., Burmistrov, S. O. and Arutyunyan, A. V. : Glutathione-dependent system of antioxidant defense in the placenta in preterm delivery. Bull. Exp. Biol. Med. 133, 442 (2002).   DOI   ScienceOn
22 Auer, T., Khoschsorur, G. A., Rabl, H., Iberer, F., Petutschnigg, B., Wasler, A. and Tscheliessnigg, K. H. : Detection of lipid peroxidation products by malondialdehyde (MDA-TBA reaction) in organ transplantation. Transplant. Proc. 27, 2749 (1995).
23 생약학교재편찬위원회. 생약학 동명사 1판, 420 (2006).
24 Al-Hesayen, A., Azevedo, E. R., Floras, J. S., Hollingshead, S., Lopaschuk, G. D. and Parker, J. D. : Selective versus nonselective beta-adrenergic receptor blockade in chronic heart failure: differential effects on myocardial energy substrate utilization. Eur. J. Heart Fail. 7, 618 (2005).   DOI   ScienceOn
25 Griffith, O. W. : Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal. Biochem. 106, 207 (1980).   DOI   ScienceOn
26 Lee, D. U., Park, C. H., Kang, S. I., Min, E. G., Han, Y. H. and Lee, C. K. : Isolation of the component transformed into blue pigments by aerobic bacteria in the fruits of gardenia jasmoides. Kor. J. Pharmacogn. 29, 204 (1998).
27 Choi, S. J., Kim, M. J., Heo, H. J., Hong, B., Cho, H. Y., Kim, Y. J., Kim, H. K., Lim, S. T., Jun, W. J., Kim, E. K. and Shin, D. H. : Ameliorating effect of Gardenia jasminoides extract on amyloid beta peptide-induced neuronal cell deficit. Mol. Cells 24, 113 (2007).
28 Shin, J. W. and Park, N. H. : Treatment of chronic hepatitis B. Korean J. Med. 77, 265 (2009).
29 Hwang, S. M., Lee, Y. J., Yoon, J. J., Lee, S. M., Kang, D. G. and Lee, H. S. : Gardenia jasminoides inhibits tumor necrosis factor-alpha-induced vascular inflammation in endothelial cells. Phytother. Res. 24, S214 (2010).   DOI   ScienceOn