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Isolation and Identification of an Unauthorized Tadalafil Analogue in a Commercial Functional Food  

Baek, Du-Jong (Department of Chemistry, College of Natural Sciences, Sangmyung University)
Publication Information
YAKHAK HOEJI / v.54, no.4, 2010 , pp. 240-243 More about this Journal
Abstract
High-performance liquid chromatography (HPLC) screening method revealed that a propolis product marketed as a functional food contained an undeclared substance similar to tadalafil, the active ingredient of the prescription drug Cialis$^{(R)}$ approved for the treatment of male erectile dysfunction. In order to identify the illegal additive, the propolis product was extracted with methylene chloride, and the extract was purified further using semipreparative HPLC. The chemical structure of the isolated substance was elucidated based on IR, LC/MS-ESI, and $^1H$- and $^{13}C$-NMR spectroscopy, which showed the characteristics similar to tadalafil. The only difference was the substitution of the methyl group at the piperazinedione ring of tadalafil to the amino group of the identified illegal additive.
Keywords
functional food; HPLC screening; illegal additive; tadalafil;
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Times Cited By KSCI : 1  (Citation Analysis)
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