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Effect of Licochalcone A on the Production of Cytokines in LPS-Activated RAW264.7 Macrophage Cells  

Lee, Gi-Sae (College of Pharmacy & Research Institute of Drug Development, Chonnam National University)
Lee, Sung-Ho (College of Pharmacy & Research Institute of Drug Development, Chonnam National University)
Cho, Young-Chang (College of Pharmacy & Research Institute of Drug Development, Chonnam National University)
Yoon, Goo (Korea Institute of Science and Technology, Gangneung Institute)
Cheon, Seung-Hoon (College of Pharmacy & Research Institute of Drug Development, Chonnam National University)
Kang, Bok-Yun (College of Pharmacy & Research Institute of Drug Development, Chonnam National University)
Publication Information
YAKHAK HOEJI / v.53, no.6, 2009 , pp. 321-327 More about this Journal
Abstract
Licochalcone A is a chalcone isolated from the roots of Glycyrrhiza inflate. In this study, we examined the effects of licochalonce A on the production of cytokines in LPS-activated macrophages. Licochalcone A inhibited the secretion of proinflammatory cytokines such as IL-1$\beta$, IL-6, and TNF-$\alpha$. The reduced secretion of proinflammatory cytokines is related to the differences in the mRNA expression of IL-1$\beta$, IL-6, and TNF-$\alpha$. Moreover, licochalcone A inhibited the mRNA expression of IL-12p40, IL-18, and IL-23p19. To investigate its mechanism, we performed gel shift assay. Licochalcone A reduced nuclear NF-${\kappa}B$ binding activity in LPS-activated RAW264.7 cells. Taken together, these results suggest that licochalcone A has anti-inflammatory effects in LPS-activated macrophages and its mechanism could be through the down-regulation of binding to the ${\kappa}B$ site.
Keywords
licochalcone A; macrophage; inflammation; cytokines;
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