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Proteomic Approach to the Cytotoxicity of 5-FU(Fluorouracil) in Colon Cancer Cells  

Lee, Seo-Young (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Song, Jin-Su (Department of Chemistry, Seoul National University)
Roh, Si-Hun (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Kim, Geun-Tae (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Hong, Soon-Sun (College of Medicine (BK21), Inha University)
Kim, Hie-Joon (Department of Chemistry, Seoul National University)
Kwon, Sung-Won (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Park, Jeong-Hill (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
Publication Information
YAKHAK HOEJI / v.53, no.3, 2009 , pp. 145-150 More about this Journal
Abstract
We evaluated cytotoxic effect based on the MTT assay and identified altered proteins in 5-FU(fluorouracil) treated HT29 cells using two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS. As proteins inducing apoptosis, siah binding protein 1 and p47 protein isoform a were up-regulated and tumor protein translationally-controlled 1 was down-regulated by 5-FU treatment. And mannose 6 phosphate receptor binding protein 1 controls DNA mismatch repair system was increased. We suggest 5-FU promotes a cytotoxicity under the action of these proteins in colon cancer cells.
Keywords
5-FU; colon cancer; proteomics; MALDI-TOF/TOF;
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