Browse > Article

Biolosical Activities of Isolated Compounds from Peucedani Radix  

Kim, Do-Hoon (College of Pharmacy, Chung-Ang University)
Han, Chi-Su (College of Pharmacy, Chung-Ang University)
Kim, Gi-Eun (College of Pharmacy, Chung-Ang University)
Kim, Jin-Hyo (College of Pharmacy, Chung-Ang University)
Kim, Sung-Gun (College of Pharmacy, Chung-Ang University)
Kim, Ho-Kyoung (Korea Institute of Oriental Medicine)
Oh, O-Jin (Department of Herbal Medicines Management, Catholic Sangji College)
Whang, Wan-Kyunn (College of Pharmacy, Chung-Ang University)
Publication Information
YAKHAK HOEJI / v.53, no.3, 2009 , pp. 130-137 More about this Journal
Abstract
In this study, isolation of antioxidative compounds was performed for development of anti-oxidizing agent. $CHCl_3$, $H_2O$, 30%, 60% MeOH, MeOH fractions were examined antioxidative activity by DPPH, test of inhibition on NO production. It was revealed that 30%, 60% MeOH and $CHCl_3$ fractions had significant antioxidative activity. In 30% MeOH and 60% MeOH, $CHCl_3$ fraction, six compounds were isolated and elucidated as adenosine(I), guanosine(II), peucedanol 7-O-$\beta$-D-apiofuranosyl(1$\rightarrow$6)-$\beta$-glucopyranoside(III), peucedanol 7-O-$\beta$-D-glucopyranoside(IV), peucedanol(V) and scopoletin(VI) by physicochemical data and spectroscopic methods. (Negative FAB-MS, $^{1}H-NMR$, $^{13}C-NMR$). The results from antioxidative activity screening for the each compound showed that compound IV was relatively superior antioxidant ability. In anti-inflammatory activation assay, compound III, IV, VI had concentration-dependent-activity and compound IV had superior anti-inflammatory ability. These results suggest that Peucedani Radix might be developed as a potent anti-oxidative, anti-inflammatory agents and ingredients for related functional foods.
Keywords
Peucedani Radix; antioxidative effect; Peucedanum japonicum;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Yasumasa, I., Izumi, M. and Yutaka, T. : Dihydropyranocoumarins from roots of Peucedanum japonicum. Phytochemistry 31(12), 4303 (1992)   DOI   ScienceOn
2 Yasumasa, I., Izumi, M. and Yutaka, T. : Dihydropyranocoumarins from Peucedanum japonicum. Phytochemistry 33(6), 1543 (1993)   DOI   ScienceOn
3 Aida, Y., Kasama, T., Takeuchi, N., Chiba, M. and Tobinaga, S. : Pharmacological activities of khellactones, compounds isolated from Peucedanum japonicum thunb. and Peucedanum praeruptorium DUNN. Exp. Clin. Pharmacol. 20(4), 343 (1998)
4 Mosmann, T. : Rapid colormetric assay for the cellular growth and survival. J. Immune. Methods 65, 55 (1983)
5 Teijeira, M., Santana, L. and Uriarte, E. : Assignment of the 13C NMR spectra of some adenine, hypoxanthine and guanine carbonucleosides. Magn. Reson. Chem. 35, 806 (1997)   DOI   ScienceOn
6 생약학교재편찬위원회 : 생약학, 동명사, p 137 (2006)
7 Zheng, M. S., Jin, W. Y., Son, K. H., Chang, H. W., Kim, H. P., Bae, K. H. and Kang, S. S. : The constituents isolated from Peucedanum japonicum thunb. and their cyclooxygenase (COX) inhibitory activity. Kor. J. Medicinal Crop. Sci. 13(2), 75 (2005)
8 Lee, S. O., Choi, S. Z., Lee, J. H., Chung, S. H., Park, S. H., Kang, H. C., Yang, E. Y., Cho, H. J. and Lee, K. R. : Antidiabetic coumarin and cyclitol compounds from Peucedanum japonicum. Arch. Pharm. Res. 27(12), 1207 (2004)   DOI   ScienceOn
9 Hata, Y. and Katsumi, N. : 일본약학잡지 88, 513 (1968)
10 Denisa, D., Weidong, H., Norbert, D. K., Monica, H. and Joseph, P. : β-Adenosine, a bioactive compound in grass chaff stimulating mushroom production. Phytochemistry 65, 181 (2004)   DOI   ScienceOn
11 Chang, Y. D., Shang, K. W. and Yang, C. W. : Cytotoxic pyranocoumarins from the aerial parts of Peucedanum japonicum. Phytochemistry 30(8), 2812 (1991)   DOI   ScienceOn
12 Hatano, T., Edamatsu, R., Hiramatus, M., Mori, A., Fujita, Y., Yasuhara, T., Yoshida, T. and Okuda, T. : Effects of the interaction of tannins with co-existing substances. IV. Effects of tannins and related polyphenols on superoxide anion radical, and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37, 2016 (1989)   DOI
13 대한 약전 제 8개정 대한 약전외 한약(생약) 규격집
14 한국한의학연구원, 한약재 관능검사기준 연구(한약재도감편), 보건복지부, 서울, p 88-89 (1999)
15 Takeuchi, N., Kasama, T., Aida, Y., Oki, J., Maruyama, I., Watanabe, K. and Tobinaga. S. : Pharmacological activities of the prenylcoumarins, developed from folk usage as a medicine of Peucedanum japonicum thunb. Chem. Pharm. Bull. (Tokyo) 39(6), 1415 (1991)   DOI   ScienceOn
16 Morioka, T., Suzui, M., Nabandith, V., Inamine, M., Aniya, Y., Nakayama, T., Ichiba, T., Mori, H. and Yoshimi. N. : The modifying effect of Peucedanum japonicum, a herb in the Ryukyu Islands, on azoxymethane-induced colon preneoplastic lesions in male F344 rats. Cancer Lett. 205(2), 133 (2004)   DOI   ScienceOn
17 Chang, Y. D., Shang, K. W. and Yang, C. W. : Cytotoxic pyranocoumarins from roots of Peucedanum japonicum. Phytochemistry 31(5), 1829 (1992)   DOI   ScienceOn
18 Huong, D. T., Choi, H. C., Rho, T. C., Lee, H. S., Lee, M. K. and Kim, Y. H. : Inhibitory activity of monoamine oxidase by coumarins from Peucedanum japonicum. Arch. Pharm. Res. 22(3), 324 (1999)   DOI
19 Whang, W. K., Lee, S. J., Kim, H. H., Cho, H. K., Lee, K. S. and Ham, I. H. : Standardization of Peucedani Radix. Kor. J. Pharmacogn 32(4), 292 (2001)
20 Shin, K. H., Kang, S. S. and Chi, H. H. : Quantitative analysis of peucedanol in Peucedanum japonicum. Kor. J. Pharmacogn 23(1), (1992)
21 Jong, T. T., Hwang, H. C., Jean, M. Y., Wu, T. S., Teng, C. M. : An antiplatelet aggregation principle and X-ray structural analysis of cis-khellactone diester from Peucedanum japonicum. J. Nat. Prod. 55(10), 1396 (1992)   DOI   PUBMED
22 Feelisch, M. and Stamler, J.: Methods in nitric oxide research. John Wiley & Sons, 492-297 (1983)
23 Park, S. Y., Hong, S. S., Han, X. H., Ro, J. S. and Whang, B. Y. :Inhibitory constituents of LPS-induced nitric oxide production form arctium lappa. Nat. Prod. Sci. 11(2), 85 (2005)
24 Ikeshiro, Y., Mase, I. and Tomita, Y. : Coumarins glycosides from Peucedanum japonicum. Phytochemistry 35(5), 1339 (1994)   PUBMED
25 김호철 : 한약약리학, 집문당, p 75 (2004)
26 Choi, H. C., Rho, T. C., Kim, B. Y., Ko, H. R., Oh, W. K., Seong, C. K., Mheen, T. I., Ahn, J. S. and Lee, H. S. : Inhibition of nitric oxide production by coumarins from Peucedanum Japonicum in LPS-Activated RAW 264.7 cells. Kor. J. Pharmacogn 30(2), 99 (1999)
27 Lee, J. W., Roh, T. C., Rho, M. C., Kim, Y. K. and Lee, H. S. : Mechanisms of relaxant action of a pyranocoumarin from Peucedanum japonicum in isolated rat thoracic aorta. Planta. Med. 68(10), 891 (2002)   DOI   ScienceOn