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Cell-cell Adhesion of Jurkat T Cells Induced by CD29 and CD98 Activation and its Application  

Kim, Byung-Hun (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University)
Cho, Jae-Youl (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University)
Publication Information
YAKHAK HOEJI / v.53, no.3, 2009 , pp. 119-124 More about this Journal
Abstract
Cell-cell adhesion managed by various adhesion molecules plays an important role in regulating functional activation of cells. This event mediates attachment of inflammatory cells to endothelial cells, interaction of antigen-presenting cells with T cells and metastatic adherence of cancer cells to epithelial tissue cells. Therefore, this cellular response is considered as one of therapeutic target to treat various cancers and inflammatory diseases. To develop proper model for evaluation of functional activation of adhesion molecules, the ability of U937 and Jurkat T cells responsive to various adhesion inducers such as phorbal-12-myristate-13-acetate (PMA), staurosporin and monoclonal antibodies to CD29, CD43 and CD98 was investigated using quantitative cell-cell adhesion assay. U937 cells made more cell-cell clusters by the treatment of antibodies to CD29 and CD43 than Jurkat T cells, while Jurkat T cells exhibited increased cell-cell adhesion ability in CD98 antibody treatment. In agreement, the surface levels of CD29 and CD98 were highly observed in U937 and Jurkat T cells, respectively. Therefore, our data suggest that Jurkat T and U937 cells can be used for model system to evaluate functional activation of adhesion molecules such as CD29 and CD98.
Keywords
cell-cell adhesion; adhesion molecules; U937 cell; Jurkat T cell;
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1 Murtaugh, M. P. and Foss, D. L. : Inflammatory cytokines and antigen presenting cell activation. Vet. Immunol. Immunopathol. 87, 109 (2002)   DOI   PUBMED   ScienceOn
2 Caulfield, J. J., Fernandez, M. H., Sousa, A. R., Lane, S. J., Lee, T. H. and Hawrylowicz, C. M. : Regulation of major histocompatibility complex class II antigens on human alveolar macrophages by granulocyte-macrophage colony-stimulating factor in the presence of glucocorticoids. Immunology 98, 104 (1999)   DOI   PUBMED
3 Woods, M. L. and Shimizu, Y. : Signaling networks regulating beta1 integrin-mediated adhesion of T lymphocytes to extracellular matrix. J. Leukoc. Biol. 69, 874 (2001)   PUBMED
4 Van Waes, C. : Cell adhesion and regulatory molecules involved in tumor formation, hemostasis, and wound healing. Head Neck 17, 140 (1995)   DOI   PUBMED   ScienceOn
5 Matsumoto, M., Shigeta, A., Miyasaka, M. and Hirata, T. : CD43 plays both antiadhesive and proadhesive roles in neutrophil rolling in a context-dependent manner. J. Immunol.181, 3628 (2008)   DOI
6 Cho, J. Y. : Chloroquine, an anti-malarial agent, acts as a novel regulator of beta 1-integrin-mediated cell-cell adhesion. Biol. Pharm. Bull. 31, 726 (2008)   DOI   PUBMED   ScienceOn
7 White, J. M. : ADAMs: modulators of cell-cell and cell-matrix interactions. Curr. Opin. Cell Biol. 15, 598 (2003)   DOI   PUBMED   ScienceOn
8 Tetley, T. D. : Inflammatory cells and chronic obstructive pulmonary disease. Curr. Drug Targets Inflamm. Allergy 4, 607 (2005)
9 Mettouchi, A. and Meneguzzi, G. : Distinct roles of beta1 integrins during angiogenesis. Eur. J. Cell Biol. 85, 243 (2006)   DOI   ScienceOn
10 Cho, J. Y., Fox, D. A., Horejsi, V., Sagawa, K., Skubitz, K. M., Katz, D. R. and Chain, B. : The functional interactions between CD98, beta1-integrins, and CD147 in the induction of U937 homotypic aggregation. Blood 98, 374 (2001)   DOI   PUBMED   ScienceOn
11 Tsurudome, M. and Ito, Y. : Function of fusion regulatory proteins (FRPs) in immune cells and virus-infected cells. Crit. Rev. Immunol. 20, 167 (2000)   PUBMED
12 Yusuf-Makagiansar, H., Anderson, M. E., Yakovleva, T. V., Murray, J. S. and Siahaan, T. J. : Inhibition of LFA-1/ICAM-1 and VLA-4/VCAM-1 as a therapeutic approach to inflammation and autoimmune diseases. Med. Res. Rev. 22, 146 (2002)   DOI   ScienceOn
13 Cho, J. Y., Kim, A. R., Joo, H. G., Kim, B. H., Rhee, M. H., Yoo, E. S., Katz, D. R., Chain, B. M. and Jung, J. H. : Cynaropicrin, a sesquiterpene lactone, as a new strong regulator of CD29 and CD98 functions. Biochem. Biophys. Res. Commun. 313, 954 (2004)
14 Kang, J. S., Yoon, Y. D., Han, M. H., Han, S. B., Lee, K., Lee, K. H., Park, S. K. and Kim, H. M. : Glabridin suppresses intercellular adhesion molecule-1 expression in tumor necrosis factor-alpha-stimulated human umbilical vein endothelial cells by blocking sphingosine kinase pathway: implications of Akt, extracellular signal-regulated kinase, and nuclear factor-kappaB/Rel signaling pathways. Mol. Pharmacol. 69, 941 (2006)   PUBMED
15 Cho, J. Y., Katz, D. R. and Chain, B. M. : Staurosporine induces rapid homotypic intercellular adhesion of U937 cells via multiple kinase activation. Br. J. Pharmacol. 140, 269 (2003)
16 Cho, J. Y., Chain, B. M., Vives, J., Horejsi, V. and Katz, D. R. : Regulation of CD43-induced U937 homotypic aggregation. Exp. Cell Res. 290, 155 (2003)   DOI   ScienceOn
17 Stonehouse, T. J., Woodhead, V. E., Herridge, P. S., Ashrafian, H., George, M., Chain, B. M. and Katz, D. R. : Molecular characterization of U937-dependent T-cell co-stimulation. Immunology 96, 35 (1999)   DOI   ScienceOn
18 Helming, L. and Gordon, S. : The molecular basis of macrophage fusion. Immunobiology 212, 785 (2007)   DOI   ScienceOn
19 Reddy, K. V. and Mangale, S. S. : Integrin receptors: the dynamic modulators of endometrial function. Tissue Cell 35, 260 (2003)   DOI   ScienceOn