Browse > Article

The Effect of Litsea japonica on the Apoptosis Induction of HL-60 Leukemia Cells  

Kim, Elvira (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Cheju National University)
Boo, Hye-Jin (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Cheju National University)
Hyun, Jae-Hee (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Cheju National University)
Kim, Sang-Cheol (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Cheju National University)
Kang, Jung-Il (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Cheju National University)
Kim, Min-Kyoung (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Cheju National University)
Yoo, Eun-Sook (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Cheju National University)
Kang, Hee-Kyoung (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Cheju National University)
Publication Information
YAKHAK HOEJI / v.53, no.1, 2009 , pp. 6-11 More about this Journal
Abstract
This study investigated the antiproliferative effect of the EtOH extract from Litsea japonica. The extract markedly inhibited the growth of HL-60 cells. When treated with the extract, several apoptosis events like as DNA fragmentation, chromatin condensation and the increase of the population of sub-G1 hypodiploid cells were observed. The extract decreased the Bcl-2 expression, whereas the Bax expression was increased. Caspase-9 and -3 were activated and poly (ADP-ribose) polymerase was cleaved. The results suggest that the antiproliferative effect of L. japonica in HL-60 appears to arise from apoptosis induction via the down-regulation of Bcl-2 and the activation of caspases.
Keywords
Litsea japonica; HL-60; apoptosis; Bcl-2; Bax; caspases;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Takeda, K., Sakurawi, K. and Ishii, H. : Components of the Lauraceae family-l. New lactonic compounds from Litsea japonica. Tetrahedron. 28, 3757 (1972)   DOI   ScienceOn
2 Carmichael, J., Degraff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell, J. B. : Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemisensitivity testing. Cancer Research 47, 944 (1987)
3 Purohit, A., Hejaz, H. A., Walden, L., MacCarthy-Morrogh, L., Packham, G., Potter, B. V. and Reed, M. J. : The effect of 2-methoxyoestrone-3-O-sulphamate on the growth of breast cancer cells and induced mammary tumours. Int. J. Cancer. 85, 584 (2000)   DOI   ScienceOn
4 Nicoletti, I., Migliorati, G., Pagliacci, M. C., Grignani, F. and Riccardi, C. : A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Methods 139, 271 (1991)   DOI   ScienceOn
5 Kroemer, G., Zamzami, N. and Susin, S. A. : Mitochondrial control of apoptosis. Immunol. Today 18, 44 (1997)   DOI   ScienceOn
6 Tanaka, H., Nakamura, T. and Ichino, K. : Butanolides from Litsea japonica. Phytochemistry 29, 857 (1990)   DOI   ScienceOn
7 Bradford, M. M. : A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248(1976)   DOI   PUBMED   ScienceOn
8 Luo, Y. and Kessel, D. : Initiation of apoptosis versus necrosis by photodynamic therapy with chloroaluminum phthalocyanine. Photochem. Photobiol. 66, 479 (1997)   DOI   ScienceOn
9 Korsmeyer, S. J., Yin, X. M., Oltvai, Z. N., Veis-Novack, D. J. and Linette, G. P. : Reactive oxyden species and the regulation of cell death by the Bcl-2 gene family. Biochem. Biophys. Acta. 1271(1), 63 (1995)   DOI   PUBMED   ScienceOn
10 Min, B. C. : Lactones from the leaves of the Litsea japonica and their anti-complement activity. J. Nat. Prod. 66(10), 1388(2003)   DOI   ScienceOn
11 Lee, S. Y., Min, B. S., Kim, J. H., Lee, J., Kim, T. J., Kim, C. S., Kim, Y. H. and Lee, H. K. : Flavonoids from the Leaves of Litsea japonica and their anti-complement activity. Phytotherapy Research 19, 273 (2005)   DOI   ScienceOn