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$18{\beta}$-Glycyrrhetinic Acid Induces Protective Anti-Candida albicans Antibody by Its Immunoadjuvant Activity  

Han, Yong-Moon (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University)
Publication Information
YAKHAK HOEJI / v.52, no.6, 2008 , pp. 494-499 More about this Journal
Abstract
The role of antibody in the fungal infections is controversial. However, our previous reports showed a certain epitope in Candida albicans cell wall (CACW) induces protective antibody. A major problem is that the epitope isolation requires tremendous time with high cost. This aspect led us to investigate a simple way inducing protective antibodies against C. albicans. In the present study, we determined if $18{\beta}$-glycyrrhetinic acid ($18{\beta}$-GA) from Glabrae Radix (a family of Leguminosae) has immunoadjuvant activity. Data displayed that the $18{\beta}$-GA suppressed proliferations of both T- and Blymphocytes at high concentrations, whereas below 20 ${\mu}M$ concentration the compound supported the proliferations. These observations indicate that $18{\beta}$-GA has immunoregulatory activity. Based on this observation, an immunoadjuvant effect was examined at the low concentration. Results from animal experiments showed that CACW combined with or without $18{\beta}$-GA produced the anti-C. albicans antiserum in mice. Nevertheless, the CACW combined with $18{\beta}$-GA formula only protected mice against disseminated candidiasis (P<0.05). These data implicate that $18{\beta}$-GA has immunoadjuvant activity, which may provoke the CACW antigen to induce protective antibody. Currently, we are investigating possible mechanism of how the $18{\beta}$-GA provokes such protective immunity against the disseminated disease.
Keywords
immunoregulation; Candida albicans cell wall; $18{\beta}$-GA; protective antibody; immunoadjuvant; disseminated candidiasis;
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