Browse > Article

Growth Inhibitory Effects of Omega-3 Unsaturated Fatty Acid against Cancer Cell Lines  

Han, Du-Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University)
Choi, Hyoung-Gyu (Department of Oral Anatomy, School of Dentistry, Wonkwang University)
Kang, Jeong-Il (Department of Physical Therapy, Daebul University)
Choi, Hwa-Jung (Natural Medicines Research Center, Korea Research Institute of Bioscience and Biotechnology)
Baek, Seung-Hwa (Department of Herbal Resources, Professional School of Oriental Medicine, Wonkwang University)
Publication Information
YAKHAK HOEJI / v.52, no.4, 2008 , pp. 264-273 More about this Journal
Abstract
The inhibitory effect of omega-3 such as linolenic acid (LNA), docosahexaenoic acid (DNA) and eicosapentaenoic acid(EPA) on the growth of normal cell lines and cancer cell lines was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyItetrazolium bromide (MTT) and 2,3-bis-2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-caboxanilide (XTT) methods. LNA was found to decrease the cell viability of human oral epithelioid carcinoma cells (KB) in the MTT assay, whereas EPA appeared to inhibit the cell adhesion activity of human skin melanoma cells (SK-MEL-3) in the XTT assay analysis. DPPH radical scavenging activity was examined on LNA, DHA and EPA at the concentration of 100 ${\mu}M$, where they showed about 53% scavenging activity. These results suggest that omega-3 unsaturated fatty acid has a potential anticancer activity.
Keywords
unsaturated fatty acid; cell viability; cell adhesion activity; cytotoxicity; omega-3; DPPH radical scavenging activity;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Lima, T. M., Kanunfre, C. C., Pompeia, C., Verlengia, R. and Curi, R. : Ranking the toxicity of fatty acids on Jurkat and Raji cells by flow cytometric analysis. Toxicol In Vitro 16, 741 (2002)   DOI   ScienceOn
2 Cario-Andre, M., Briganti, S., Picardo, M., Nikaido, O., de Verneuil, H. and Taieb, A. : Polyunsaturated fatty acids partially reproduce the role of melanocytes in the epidermal melanin unit. Exp. Dermatol. 14, 194 (2005)   DOI   ScienceOn
3 Umamaheswari, M., Asokkumar, F., Rathidevi, R., Sivashanmugam, A. T., Subhadradevi, V. and Ravi, T. K. : Antiulcer and in vitro antioxidant activities of Jasminum grandiflorum L. J. Ethnopharmacol. 110, 464 (2007)   DOI   ScienceOn
4 Chi, T. Y., Chen, G. G. and Lai, P. B. : Eicosapentaenoic acid induces Fas-mediated apoptosis through a p53-dependent pathway in hepatoma cells. Cancer J. 10, 190 (2004)   DOI   ScienceOn
5 Zimmermann, K. C., Bonzon, C. and Green, D. R. : The machinery of programmed cell death. Pharmacol. 92, 57 (2001)
6 Sun, S. Y. : Apoptosis induction by chemopreventive agents. Drug News Perspect 14, 75 (2001)   PUBMED
7 Larsson, S. C., Kumlin, M., Ingelman-Sundberg, M. and Wolk, A. : Dietary long-chain n-3 fatty acids for the prevention of cancer: a review of potential mechanisms. Am. J. Clin. Nutr. 79, 935 (2004)   DOI   PUBMED
8 Mosmann, T. : Rapid colorimetric assays for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55 (1983)   DOI   PUBMED   ScienceOn
9 Vecchini, A., Ceccarelli, V., Susta, F., Caligiana, P., Orvietani, P., Binaglia, L., Nocentini, G., Riccardi, C., Calviello, G., Palozza, P., Maggiano, N. and Di Nardo, P. : Dietary alpha-linolenic acid reduces COX-2 expression and induces apoptosis of hepatoma cells. J. Lipid Res. 45, 308 (2004)   DOI   ScienceOn
10 Menendez, J. A., del Mar Barbacid, M., Montero, S., Sevilla, E., Escrich, E., Solanas, M., Cortes-Funes, H. and Colomer, R. : Effects of gamma-linolenic acid and oleic acid on paclitaxel cytotoxicity in human breast cancer cells. Eur. J. Cancer 37,402 (2001)   PUBMED
11 Menendez, J. A., Lupu, R. and Colomer, R. : Exogenous supplementation with omega-3 polyunsaturated fatty acid docosahexaenoic acid (DHA; 22:6n-3) synergistically enhances taxane cytotoxicity and downregulates Her-2/neu (c-erbB-2) oncogene expression in human breast cancer cells. Eur. J. Cancer Prev. 14, 263 (2005)   DOI   ScienceOn
12 Li, J. Q., Chen, R. C., Cai, K. X. and Ye, Z. Y. : Apoptosis of human gastric cancer cell induced by photochemical riboflavin. Ai Zheng 22, 253 (2003)   PUBMED
13 Jones, R., Adel-Alvarez, L. A., Alvarez, O. R., Broaddus, R. and Das, S. : Arachidonic acid and colorectal carcinogenesis. Mol. Cell Biochem. 253, 141 (2003)   DOI   PUBMED   ScienceOn
14 Bougnoux, P. and Menanteau, J. Dietary fatty acids and experimental carcinogenesis. Bull. Cancer 92, 685 (2005)   PUBMED
15 Han, D. S., Chun, J. W., Jeon, S. W. and Baek, S. H. : The inhibitory effect of ferulic acid and related phenolic compounds against cancer cell lines. J. Pharm. Soc. Kor. 49, 365 (2005)   과학기술학회마을
16 Dommels, Y. E., Haring, M. M., Keestra, N. G., Alink, G. M., van Bladeren, P. J. and van Ommen, B. : The role of cyclooxygenase in n-6 and n-3 polyunsaturated fatty acid mediated effects on cell proliferation, PGE(2) synthesis and cytotoxicity in human colorectal carcinoma cell lines. Carcinogenesis 24, 385 (2003)   DOI   ScienceOn
17 Hatano, T., Edamatsu, R., Hiramatus, M., Mori, A., Fujita, Y., Yasuhara, T., Yoshida, T. and Okuda, T. : Effects of the interaction of tannins with co-existing substances. IV. Effects of tannins and related polyphenols on superxide anion radical and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37, 2016 (1989)   DOI
18 Sravan, K. G. and Das, U. N. : Cytotoxic action of alphalinolenic and eicosapentaenoic acids on myeloma cells in vitro. Prostaglandins Leukot Essent Fatty Acids 56, 285 (1997)   DOI   ScienceOn
19 Horrobin, D. F. : Phospholipid metabolism and depression: the possible roles of phospholipase A2 and coenzyme Aindependent transacylase. Hum. Psychopharmacol. 16, 45 (2001)   DOI   ScienceOn
20 Liu, G., Bibus, D. M., Bode, A. M., Ma, W. Y., Holman, R. T. and Dong, Z. : Omega 3 but not omega 6 fatty acids inhibit AP-1 activity and cell transformation in JB6 cells. Proc. Natl. Acad. Sci. USA 98, 7510 (2001)
21 Ghosh, J. : Rapid induction of apoptosis in prostate cancer cells by selenium: reversal by metabolites of arachidonate 5- lipoxygenase. Biochem. Biophys. Res. Commun. 315, 624 (2004)   DOI   PUBMED   ScienceOn
22 Jiang, W. G., Redfern, A., Bryce, R. P. and Mansel, R. E. : Peroxisome proliferator activated receptor-gamma (PPARgamma) mediates the action of gamma linolenic acid in breast cancer cells. Prostaglandins Leukot. Essent. Fatty Acids 62, 119 (2000)   DOI   ScienceOn
23 Jo, E. H., Hong, H. D., Ahn, N. C., Jung, J. W., Yang, S. R., Park, J. S., Kim, S. H., Lee, Y. and Kang, K. S. : Modulations of the Bcl-2/Bax family were involved in the chemopreventive effects of licorice root (Glycyrrhiza uralensis Fisch) in MCF-7 human breast cancer cell. J. Agric. Food Chem. 52, 1715 (2004)   DOI   ScienceOn
24 Wirfalt, E., Mattisson, I., Gullberg, B., Johansson, U., Olsson, H. and Berglund, G. : Postmenopausal breast cancer is associated with high intakes of omega6 fatty acids (Sweden). Cancer Causes Control 13, 883 (2002)   DOI   ScienceOn
25 Motaung, E., Prinsloo, S. E., van Aswegen, C. H., du Toit, P. J., Becker, P. J. and du Plessis, D. J. : Cytotoxicity of combined essential fatty acids on a human prostate cancer cell line. Prostaglandins Leukot. Essent. Fatty Acids 61, 331 (1999)   DOI   ScienceOn
26 Roynette, C. E., Calder, P. C., Dupertuis, Y. M. and Pichard, C. : n-3 polyunsaturated fatty acids and colon cancer prevention. Clin. Nutr. 23, 139 (2004)   DOI   ScienceOn
27 Igarashi, M. and Miyazawa, T. D. : Conjugated eicosapentaenoic acid and conjugated docosahexaenoic acid induce apoptosis via lipid peroxidation in cultured human tumor cells. Biochem. Biophys. Res. Commun. 270, 649 (2000)   DOI   ScienceOn
28 Takahira, K., Sano, M., Arai, H. and Hanai, H. : Apoptosis of gastric cancer cell line MKN45 by photodynamic treatment with photofrin. Lasers, Med. Sci. 19, 89 (2004)   DOI   ScienceOn