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Isolation of Flavonoids from Carthami Flos and their Antioxidative Activity  

Chung, Sung-Hee (College of Pharmacy, Chung-Ang University)
Moon, Ye-Ji (College of Pharmacy, Chung-Ang University)
Kim, Sung-Gun (College of Pharmacy, Chung-Ang University)
Kim, Kyoung-Young (College of Pharmacy, Chung-Ang University)
Lee, Kyoung-Tae (College of Pharmacy, Chung-Ang University)
Kim, Ho-Kyoung (Korea Institute of Oriental Medicine)
Whang, Wan-Kyunn (College of Pharmacy, Chung-Ang University)
Publication Information
YAKHAK HOEJI / v.52, no.4, 2008 , pp. 241-251 More about this Journal
Abstract
In this study, isolation of antioxidative compounds was performed for development of anti-oxidizing agent. $CHCl_{3}$, $H_{2}O$, 30%, 60% MeOH, MeOH fractions were examined antioxidative activity by DPPH method, TBARS assay, and SOD like activity. It was revealed that 30%, 60% MeOH fractions had significant antioxidative activity. From 30%, 60% MeOH fraction, nine compounds were isolated and elucidated kaempferol $3-O-{\alpha}-L-rhamnopyranosyl$ $(1{\rightarrow}6)-{\beta}-D-glucopyranoside$ (1), quercetin $7-O-{\beta}-D-glucopyranoside$ (II), quercetin $3-O-{\alpha}-L-rhamnopyranosyl$ $(1{\rightarrow}6)$ ${\beta}-D-glucopyranoside(rutin)$ (III), 6-hydroxykaempferol $3-O-{\beta}-D-glucopyranoside$ (lV), kaempferol $3-O-{\beta}-D-glucopyranosyl$ $(1{\rightarrow}2)$ ${\beta}-D-glucopyranoside$ (V), kaempferol $3-O-{\beta}-D-glucopyranoside$ (VI), luteolin (VII), quercetin $3-O-{\beta}-D-glucopyranoside$ (VIII), apigenin $7-O-{\beta}-D-glucuronopyranoside$ (IX) through physicochemical data and spectroscopic methods (Negative FAB-MS, $^1H-NMR$, $^{13}C-NMR$). Entirely, all compounds had similar antioxidative activity, but more OH group had more antioxidative activity.
Keywords
Carthami flos; antioxidative effect;
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Times Cited By KSCI : 1  (Citation Analysis)
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