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Inhibitory Effects on Melanin Production in B16 Melanoma Cells of Sedum sarmentosum  

Sim, Gwan-Sub (R&D Center, Hanbul Cosmetics Co. Ltd.)
Kim, Jin-Hwa (R&D Center, Hanbul Cosmetics Co. Ltd.)
Lee, Bum-Chun (Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tupper Medical Building)
Lee, Dong-Hwan (R&D Center, Hanbul Cosmetics Co. Ltd.)
Lee, Geun-Soo (R&D Center, Hanbul Cosmetics Co. Ltd.)
Pyo, Hyeong-Bae (R&D Center, Hanbul Cosmetics Co. Ltd.)
Publication Information
YAKHAK HOEJI / v.52, no.3, 2008 , pp. 165-171 More about this Journal
Abstract
To develop effective skin whitening agents, we tested natural herbal extracts for their melanogenic inhibitory activities. Sedum samentosum was selected for its inhibitory effect on melanogenesis in B16 melanoma cells. Ethanolic extract of S. samentosum (SSE) was evaluated for antioxidative effect and tyrosinase inhibitory activity of melanogenesis. We investigated the changes in protein level and mRNA level of tyrosinase, tyrosinase related protein (TRP)-1 and TRP-2 by using western blotting and RT-PCR, respectively. SSE showed scavenging activities of free radicals and reactive oxygen species (ROS) with the $IC_{50}$ of 342.7 $\mug/ml$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 64.69 $\mug/ml$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. SSE treatment suppressed the biosynthesis of melanin up to 46% and reduced tyrosinase activity up to 51% at 100 $\mug/ml$ in B16 melanoma cells. The tyrosinase activity and tyrosinase expression in B16 melanoma cells were reduced in a dose-dependent manner by SSE. Also, SSE was able to significantly inhibit tyrosinase and TRP-1 expression in mRNA level. These results suggest that SSE inhibited melanin production which may be dependent on tyrosinase activity and expression in B16 melanoma cells, and an effective whitening agent for the skin.
Keywords
Sedum sarmentosum; B16 melanoma; melanin; tyrosinase; tyrosinase related protein;
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Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Seiberg, M. : Kerationcyte-melanocyte interaction during melanosome transfer. Piment Cell Res. 14, 236 (2001)   DOI   ScienceOn
2 Sim, G. S., Kim, J. H., Lee, D. H., Na, Y., Lee, G. S. and Pyo, H. B. : Sedum sarmentosum enhances hyaluronan synthesis in transformed human keratinocytes and increases water content in human skin. J. Soc. Cosmet. Scientists Korea 33, 17 (2007)   과학기술학회마을
3 Blois, M. S. : Antioxidation determination by the use of a stable free radical. Nature 181, 1199 (1958)   DOI   ScienceOn
4 Hatano, T. : Constituents of natural medicines with scavenging effects on active oxygen species tannins and related polyphenols. Natural Medicines 49, 357 (1995)
5 Steingrimsson, E., Copeland, N. G. and Jenkins, N. A. : Melanocytes and the microphtalmia transcription factor network. Annu. Rev. Genet. 38, 365 (2004)   DOI   ScienceOn
6 Bertolotto, C., Abbe, P., Hemesath, T. J., Bille, K., Fisher, D. E., Ortonne, J. P. and Ballotti, R. : Microphthalmia gene product as a signal transducer in cAMP-induced differentiation of melanocytes. J. Cell Biol. 142, 827 (1998)   DOI   ScienceOn
7 Cho, Y. H., Lee, B. C., Kim, J. H., Kim, J. H., Pyo, H. B., Zhang, Y. H. and Park, H. D. : Effect of Aetemisia anomala S. Moore on antioxidant activity and melanogenesis. Korean J. Pharmacognosy 36, 273 (2005)   과학기술학회마을
8 Tachibana, M. : MITF: A stream flowing for pigment cells. Pigment Cell Res. 13, 230 (2000)   DOI   ScienceOn
9 Hearing, V. J. and Tsukamoto, K. : Biochemical control of melanogenesis and melanosomal organization. J. Invest. Dermatol. Symp. Proc. 4, 24 (1999)
10 Furuno, K., Akasako, T. and Sugihara, N. : The contribution of the pyrogallol moiety to the superoxide radical scavenging activity of flavonoids. Biol. Pharm. Bull. 25, 19 (2002)   DOI   ScienceOn
11 Busca, R. and Ballotti, R. : Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res. 13, 60 (2000)   DOI   ScienceOn
12 Oh, H. C., Kang, D. G., Kwon, J. W., Kwon, T. O., Lee, S. Y., Lee, D. B. and Lee, H. S. : Isolation of angiotensin converting enzyme (ACE) inhibitory flavonoids from Sedum sarmentosum. Biol. Pharm. Bull. 27, 2035 (2004)   DOI   ScienceOn
13 Hosoi, J., Abe, E., Suda, T. and Kuroki, T. : Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 45, 1474 (1985)   PUBMED
14 Pawelk, J. : Melanoma cells in culture. Methods Enzymol. 58, 564 (1978)
15 Fisher, D. E. : Microphthalmia: A signal responsive transcriptional regulator in development. Pigment Cell Res. 13(Suppl. 8), 145 (2000)   DOI   ScienceOn
16 Bentley, N. J., Eisen, T. and Goding, C. R. : Melanocyte-specific expression of the human tyrosinase promoter: activation by the microphthalmia gene product and role of the initiator. Mol. Cell. Biol. 14, 7996 (1994)   DOI   PUBMED
17 Hill, H. Z., Li, W., Xin, P. and Mitchell, D. L. : Melanin: a two edged sword? Pigment Cell Res. 10, 158 (1997)   DOI   ScienceOn
18 Aimin, H., Mingshi, W., Hongyan, H., Decheng, Z. and Lee, K. H. : Hepatoprotectice triterpenes from Sedum sarmentosum. Phytochemistry 49, 2607 (1998)   DOI   ScienceOn
19 Pomerantz, S. H. : Separation, purification, and properties of two tyrosinase from hamster melanoma. J. Biol. Chem. 238, 2351 (1963)   PUBMED
20 Cabanes, J., Chazarra, S. and Garcia-Carmona, F. : Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor of catecholase activity of tyrosinase. J. Pharm. Pharmacol. 46, 982 (1994)   DOI   PUBMED   ScienceOn
21 Marmol, V. and Beermann, F. : Tyrosinase and related protein in mammalian pigmentation. FEBS Letters 381, 165 (1996)   DOI   ScienceOn
22 Yoo, H. S., Kim, J. K. and Kim, S. J. : The inhibitory effect on the melanin synthesis in B16/F10 mouse melanoma cells by Sosa quelpaertensis leaf extract. J. Life Sciences 17, 873 (2007)
23 Mosmann, T. : Rapid colorimetric assay for the cellular growth and survival : application to proliferation and cytotoxic assay. J. Immun. Methos 65, 55 (1983)   DOI   ScienceOn
24 Lee, K. E., Sim, G. S., Kim, J. H., Park, S. M., Lee. B. C., Yun, Y. P., Zhang, Y. H. and Pyo, H. B. : Effects of the Scirpi rhizoma on antixodiation and melanogenesis. Yakhak Hoeji 48, 323 (2004)