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Alteration of Cytosolic Ca$^{2+}$ Signal by Cryopreservation in Pig Sperm  

Lee, Sun-Woo (College of Pharmacy, Chungnam National University)
Li, Yu-Hua (College of Pharmacy, Chungnam National University)
Kim, Joon-Chul (College of Pharmacy, Chungnam National University)
Myung, Pyung-Keun (College of Pharmacy, Chungnam National University)
Park, Chang-Sik (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Woo, Sun-Hee (College of Pharmacy, Chungnam National University)
Publication Information
YAKHAK HOEJI / v.50, no.6, 2006 , pp. 409-414 More about this Journal
Abstract
Although mammalian sperms are cryopreserved for in vitro fertilization a process of cryopreservation decreases the fertility. Acrosome reaction requires depolarization-induced Ca$^{2+}$ influx and Ca$^{2+}$ releases from the Ca$^{2+}$ stores. To examine whether the cellular Ca$^{2+}$ mobilization is altered by a sperm cryopreservation we compared cytosolic Ca$^{2+}$ signals between fresh and cryopreserved pig sperms using confocal Ca$^{2+}$ imaging. The magnitudes of depolarization induced Ca$^{2+}$ increases were significantly smaller in cryopreserved sperms. Exposures to 10 mM caffeine or 5 ${\mu}$M thapsigargin elicited less Ca$^{2+}$ increases in the cryopreserved sperms compared to fresh sperms. In addition, progesterone-trig-gered Ca$^{2+}$ rises, that are thought to enhance acrosome reaction, were completely abolished in the cryopreserved sperms. These results suggest that storage and(/or) release of Ca$^{2+}$ from the intracellular Ca$^{2+}$ stores in pig sperms are significantly impaired by the process of cryopreservation.
Keywords
pig sperm; cryopreservation; cytosolic Ca$^{2+}$;
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