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Hepatoprotective Activities of Glycyrrhizin and Baicalin in Primary Cultured Rat Hepatocytes  

Kim, Sung-Hwa (College of Pharmacy, Sungkyunkwan University)
Cheon, Ho-Jun (College of Pharmacy, Sungkyunkwan University)
Park, Jin-Gu (College of Pharmacy, Sungkyunkwan University)
Kim, Yeong-Sik (College of Pharmacy, Seoul National University)
Kang, Sam-Sik (College of Pharmacy, Seoul National University)
Xu, Guang-Hua (College of Pharmacy, Yeungnam University)
Lee, Seung-Ho (College of Pharmacy, Yeungnam University)
Son, Kun-Ho (Department of Food Science and Nutrition, Andong National University)
Lee, Sun-Mi (College of Pharmacy, Sungkyunkwan University)
Publication Information
YAKHAK HOEJI / v.50, no.6, 2006 , pp. 358-366 More about this Journal
Abstract
The aim of this study was to investigate the protective effects of glycynhizin, active glycosides of Glycyrrhizae Radix, and baicalin, bioactive flavonoid isolated from Scutellariae Radix, on hepatocyte injury induced by carbon tetrachloride(CCl$_4$, 10 mM), tert-butyl hydroperoxide (TBH, 0.5 mM), and D-galactosamine (GaIN, 30 mM). Primary cultures of rat hepatocyte (18 hr cultured) were treated with CCl$_4$, TBH, or GaIN and various concentrations (0.1, 1, 10, and 100 ${\mu}$M) of glycyrrhizin or baicalin. Activity was accessed by determining the release of lactate dehydrogenase (LDH) and aminotransferses. CCl$_4$ significantly increased the levels of LDH, alanine aminotransferase (ALT), and aspartate aminotransferase(AST) and these increases were prevented by baicalin concentrations of 0.1,1, and 100 ${\mu}$M. The increases in ALT and AST levels were reduced by glycyrrhizin concentration of 100 ${\mu}$M. The level of LDH was markedly increased by TBH, and this increase was reduced by both glycyrrhizin and baicalin. ALT and AST levels were increased by TBH, which were prevented by glycynhizin and bacalin, respectively: GaIN markedly increased the levels of LDH, ALT and AST These increases was significantly reduced by both glycyrrhizin and baicalin. These results suggest that glycynhizin and baicalin possess the hepatoprotective activity.
Keywords
glycyrrhizin; baicalin; hepatoprotective acivity; primary cultured hepatocyte;
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