Browse > Article

Vasorelaxing Effect of Isoflavonoids Via Rho-kinase Inhibition in Agonist-Induced Vasoconstriction  

Je, Hyun-Dong (Department of Pharmacology, College of Pharmacy, Catholic University of Daegu)
Publication Information
YAKHAK HOEJI / v.50, no.4, 2006 , pp. 293-299 More about this Journal
Abstract
The aim of present study was to investigate the possible influence of Rho-kinase inhibition on the plant-derived estrogen-like compounds-induced arterial relaxation. Agonist- or depolarization-induced vascular smooth muscle contractions involve the activation of Rho-kinase pathway. However there are no reports addressing the question whether this pathway is involved in genistein-or daidzein-induced vascular relaxation in rat aortae precontracted with phenylephrine or thromboxane $A_2$ mimetic U-46619. We hypothesized that Rho-kinase inhibition plays a role in vascular relaxation evoked by genistein or daidzein in rat aortae. Endothelium-intact and denuded arterial rings from male Sprague-Dawley rats were used and isometric contractions were recorded using a computerized data acquisition system. Genistein concentration-dependently inhibited phenylephrine or thromboxane $A_2-induced$ contraction regardless of endothelial function. Surprisingly, in the agonists-induced contraction, similar results were also observed in aortae treated with daidzein, the inactive congener for protein tyrosine kinase inhibition, suggesting that Rho-kinase might act upstream of tyrosine kinases in phenylephrine-induced contraction. In conclusion, in the agonists-precontracted rat aortae, genistein and daidzein showed similar relaxant response regardless of tyrosine kinase inhibition or endothelial function.
Keywords
daidzein; genistein; vasoconstriction; phenylephrine; thromboxane $A_2$ mimetic; tyrosine kinase; Rho kinase;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Williams, J. K. and Clarkson, T. B. : Dietary soy isoflavones inhibit in vivo constrictor responses of coronary arteries to collagen-induced platelet activation. Coron. Artery Dis. 9, 759 (1998)   DOI   ScienceOn
2 Akiyama, T., Ishida, J., Nakagawa, S., Ogawara, H., Watanabe, S., Itoh, N., Shibuya, M. and Fukami, Y. : Genistein, a specific inhibitor of tyrosine- specific protein kinases. J. Biol. Chem. 262, 5592 (1987)
3 Somlyo, A. P. and Somlyo, A. V. : Signal transduction and regulation in smooth muscle. Nature 372, 231 (1994)   DOI   ScienceOn
4 Uehata, M., Ishizaki, T., Satoh, H., Ono, T., Kawahara, T.,Morishita, T., Tamakawa, H., Yamagami, K., Inui, J., Maekawa, M. and Narumiya, S. : Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature 389, 990-994 (1997)   DOI   ScienceOn
5 Davis, M. J., Wu, X., Nurkiewicz, T. R., Kawasaki, J., Gui, P., Hill, M. A. and Wilson, E. : Regulation of ion channels by protein tyrosine phosphorylation. Am. J. Physiol. 281, H1835 (2001)
6 Sakurada, S., Takuwa, N., Sugimoto, N., Wang, Y., Seto, M., Sasaki, Y. and Takuwa, Y. : Ca2+-dependent activation of Rho and Rho-kinase in membrane depolarization-induced and receptor stimulation-induced vascular smooth muscle contraction. Circ. Res. 93, 548 (2003)   DOI   ScienceOn
7 Anderson, J. W., Johnstone, B. W. and Cook-Newell, M. E. : Meta- analysis of the effects of soy protein intake on serum lipids. N. Engl. J. Med. 333, 276 (1995)   DOI   ScienceOn
8 Leung, T., Manser, E., Tan, L. and Lim, L. : A novel serine/ threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes. J. Biol. Chem. 270, 29051 (1995)   DOI
9 Kuiper, G. G., Lemmen, J. G., Carlsson, B., Corton, J. C., Safe, S. H., Van der Saag, P. T., Van der Burg, B. and Gustafsson, J. A. : Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta. Endocrinology 139, 4252 (1998)   DOI   ScienceOn
10 Somlyo, A. P. and Somlyo, A. V. : From pharmacomechanical coupling to G-proteins and myosin phosphatase. Acta. Physiol. Scand. 164, 437 (1998)   DOI   ScienceOn
11 Hsieh, C. Y., Santell, R. C., Haslam, S. Z. and Helferich, W. G. : Estrogenic effects of genistein on the growth of estrogen receptor-positive human breast cancer (MCF-7) cells in vitro and in vivo. Cancer Res. 58, 3833 (1998)
12 Liu, C. Y. and Sturek, M. : Attenuation of endothelin-1-induced calcium response by tyrosine kinase inhibitors in vascular smooth muscle cells. Am. J. Physiol. 270, C1825 (1996)   DOI
13 Wilson, D. P., Susnjar, M., Kiss, E., Sutherland, C. and Walsh, M. P. : Thromboxane$ A_2$-induced contraction of rat caudal arteial smooth muscle involves activation of $Ca^{2+}$ entry and $Ca^{2+}$ sensitization: Rho-associated kinase-mediated phosphorylation of MYPT1 at Thr-855, but not Thr-697. Biochem. J. 389, 763 (2005).p   DOI   ScienceOn
14 Kitazawa, T., Masuo, M. and Somlyo, A. P. : Protein-mediated inhibition of myosin light-chain phosphatase in vascular smooth muscle. Proc. Natl. Acad. Sci. USA 88, 9307 (1991)
15 Matsui, T., Amano, M., Yamamoto, T., Chihara, K., Nakafuku, M., Ito, M., Nakano, T., Okawa, K., Iwamatsu, A. and Kaibuchi, K. : Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho. EMBO J. 15, 2208 (1996)
16 Gohla, A., Schultz, G. and Offermanns, S. : Roles for G(12)/G(13) in agonist-induced vascular smooth muscle cell contraction. Circ. Res. 87, 221 (2000)   DOI   ScienceOn
17 Hodgson, J. M., Croft, K. D., Puddey, L. B., Mori, T. A. and Beilin, L. J. : Soybean isoflavonoids and their metabolic products inhibit in vitro lipoprotein oxidation in serum. J. Nutr. Biochem. 7, 664 (1996)   DOI   ScienceOn
18 Mitchell, J. H. and Collins, A. R. : Effects of a soymilk supplement on plasma cholesterol levels and oxidative DNA damage in men-a pilot study. Eur. J. Nutr. 38, 143 (1999)   DOI   ScienceOn
19 Low, A. M. : Role of tyrosine kinase on $Ca^{2+}$ entry and refilling of agonist-sensitive $Ca^{2+}$ stores in vascular smooth muscles. Can. J. Physiol. Pharmacol. 74, 298 (1996)   DOI   ScienceOn
20 Somlyo, A. P. and Somlyo, A. V. : Signal transduction and regulation in smooth muscle. Nature 372, 231 (1994)   DOI   ScienceOn
21 Martin, P. M., Horwitz, K. B., Ryan, D. S. and McGuire, W. L. : Phytoestrogen interaction with estrogen receptors in human breast cancer cells. Endocrinology 103, 1860 (1978)   DOI   ScienceOn
22 Hollenberg, M. D. : Tyrosine kinase pathways and the regulation of smooth muscle contractility. Trends Pharmacol. Sci. 15, 108 (1994)   DOI   ScienceOn