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Apoptotic Effect of Ethanol Extracts of Bojungbangamtang and Acidic Polysaccharide of Korea Red Ginseng in a MCF7/adR Multidrug-resistance Breast Cancer Cells  

Ahn, Gyu-In (Graduate School of East-West Medical Science, Kyunghee University)
Park, Cheol-Hwan (Graduate School of East-West Medical Science, Kyunghee University)
Lee, Eun-Ok (Graduate School of East-West Medical Science, Kyunghee University)
Lee, Hyo-Jung (Graduate School of East-West Medical Science, Kyunghee University)
Lee, Jae-Ho (Graduate School of East-West Medical Science, Kyunghee University)
Kim, Kwan-Hyun (Graduate School of East-West Medical Science, Kyunghee University)
Rhee, Yun-Hee (Graduate School of East-West Medical Science, Kyunghee University)
Jang, Yu-Sung (Graduate School of East-West Medical Science, Kyunghee University)
Kim, Sang-Tae (Nano-Bio of Research Institute, Division of Nanoscience, Ewah Womans University)
Kim, Sung-Hoon (Graduate School of East-West Medical Science, Kyunghee University,College of Oriental Medicine, Kyunghee University)
Publication Information
YAKHAK HOEJI / v.50, no.4, 2006 , pp. 272-277 More about this Journal
Abstract
This study was undertaken to determine whether the 9 herbal complex induces apoptosis in human breast cancer MCF-7 cells and adriamycin-resistant MCF7/adR cells. Ethanol extracts of Bojungbangamtang (BBTE) and acidic polysaccharide of Red Ginseng (GIN) induced cell death in both MCF-7 and MCF7/adR cells. Ethanol extracts of Bojungbangamtang and acidic polysaccharide of Red Ginseng also induced $G_2/M$ cell cycle arrest and increased TUNEL positive cells in MCF7/adR cells. In addition, flow cytometric analysis revealed the decreased expression of P-glycoprotein (P-gp) in ethanol extracts of Bojungbangamtang and acidic polysaccharide of Red Ginseng treated MCF7/adR cells. Similarly, decreased protein levels of P-glycoprotein and multidrug resistance associated proteins-1 were also determined by immunocytometry in ethanol extracts of Bojungbangamtang treated MCF7/adR cells. Taken together these data indicate that ethanol extracts of Bojungbangamtang and acidic polysaccharide of Red Ginseng inhibit the function of ABC transporters such as multi drug resistance associated proteins (MRPs) and P-glycoprotein as well as induce apoptosis in MCF7/adR cells. Thus, these data suggest that ethanol extracts of Bojungbangamtang and polysaccharide of Red Ginseng can be candidates for the treatment of multidrug-resistant MCF7/adR cells.
Keywords
BBTE; GIN; multidrug-resistance; P-gp; MRP-1; MCF7/adR; apoptosis;
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