Browse > Article

The Identification of Proteins Interacting with CD1d  

Hwang, Kwang-Woo (College of Pharmacy, Chung-Ang University)
Chun, Tae-Hoon (College of Medicine, Hanyang University)
Publication Information
YAKHAK HOEJI / v.50, no.4, 2006 , pp. 263-267 More about this Journal
Abstract
CD1d is an unique antigen presenting molecule which provides antigenic repertoires to NKT cells. To examine molecules required for CD1d antigen presentation, we determined an interaction between CD1d and several endoplasmic reticulum (ER) resident molecular chaperones by co-immunoprecipitation. Results indicated that calnexin and calreticulin seem to be bound to mouse CD1d, but TAP and tapasin do not bind. Further, we screened an yeat two hybrid system to identify proteins that help mouse CD1d transportation in the cytosol. We found that two proteins, heat shock protein a sub-unit $(Hsp90{\alpha})$ and protein kinase C and casein kinase substrate in neurons 3 (PACSIN-3), interact with CD1d. Future study will be focus on the role of these molecules during the CD1d antigen presentation.
Keywords
antigen presenting molecule; CD1d; immunoprecipitation; molecular chaperone; yeast two hybrid screening;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bendelac, A., Rivera, M. N., Park, S. H. and Roark, J. H. : Mouse CD1-specific NK1.1+ T cells: development, specificity, and function. Annu. Rev. Immunol. 15, 535 (1997)   DOI   ScienceOn
2 Kawano, T., Cui, J., Koezuka, Y., Toura, I., Kaneko, Y., Motoki, K., Ueno, H., Nakagawa, R., Sato, H., Kondo, E., Koseki, H. and Taniguchi, M. : CD1d-restricted and TCR-mediated activation of VSemin. Immunol.14 NKT cells by glycosylceramides. Science 278, 1626 (1997)   DOI
3 Smith-Jones, P. M., Solit, D. B., Akhurst, T., Afroze, F., Rosen, N. and Larson, S. M. : Imaging the pharmacodynamics of HER2 degradation in response to Hsp90 inhibitors. Nat. Biotechnol. 22, 701 (2004)   DOI   ScienceOn
4 Pamer, E. and Cresswell, P. : Mechanisms of MHC classIrelated antigen processing. Annu. Rev. Immunol. 16, 323 (1998)   DOI   ScienceOn
5 Taniguchi, M. and Nakayama, T. : Recognition and function of V$\alpha$14 NKT cells. Semin. Immunol. 12, 543 (2000)   DOI   ScienceOn
6 Bannai, M., Kawamura, T., Naito, T., Kameyama, H., Abe, T., Kawamura, H., Tsukada, C., Watanabe, H., Hatakeyama, K., Hamada, H., Nishiyama, Y., Ishikawa, H., Takeda, K., Okumura, K., Taniguchi, M. and Abo, T. : Abundance of unconventional CD8(+) natural killer T cells in the large intestine. Eur. J. Immunol. 31, 3361 (2001)   DOI   ScienceOn
7 Crompton, T., Pircher, H. and MacDonald, H. R. : CD4+8- thymocytes bearing major histocompatibility complex class Irestricted T cell receptors: evidence for homeostatic control of early stages of CD4/CD8 lineage development. J. Exp. Med. 176, 903 (1992)   DOI   ScienceOn
8 Hammond, K. J. and Godfrey, D. I. : NKT cells: Potential targets for autoimmune disease therapy- Tissue Antigens 59, 353 (2002)   DOI   ScienceOn
9 Beckman, E. M., Porcelli, S. A., Morita, C. T., Behar, S. M., Furlong, S. T. and Brenner, M. B. : Recognition of a lipid antigen by CD1-restricted $\alpha\beta$+T cells. Nature 372, 691 (1994)   DOI
10 Eberl, G., Lees, R., Smiley, S. T., Taniguchi, M., Grusby, M. J. and MacDonald, H. R. : Tissue-specific segregation of CD1ddependent and CD1d-independent NK T cells. J. Immunol. 162, 6410 (1999)
11 Modregger, J., Ritter, B., Witter, B., Paulsson, M. and Plomann M. : All three PACSIN isoforms bind to endocytic proteins and inhibit endocytosis. J. Cell Sci. 113, 4511 (2000)
12 Chiu, Y. H., Park, S. H., Benlagha, K., Forestier, C., Jayawardena-Wolf, J., Savage, P. B., Teyton, L. and Bendelac, A. : Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail-truncated CD1d. Nat. Immunol. 3, 55 (2002)   DOI   ScienceOn
13 Cui, J., Shin, T., Kawano, T., Sato, H., Kondo, E., Toura, I., Kaneko, Y., Koseki, H., Kanno, M. and Taniguchi, M. : Requirement for V$\alpha$14 NKT cells in IL-12-mediated rejection of tumors. Science 278, 1623 (1997)   DOI
14 Prigozy, T. I., Sieling, P. A., Clemens, D., Stewart, P. L., Behar, S. M., Porcelli, S. A., Brenner M. B., Modlin, R. L. and Kronenberg, M. : The mannose receptor delivers lipoglycan antigens to endosomes for presentation to T cells by CD1b molecules. Immunity 6, 187 (1997)   DOI   ScienceOn