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Effect of Fresh Rehmanniae Radix Methanol Extracts on the Production of Cytokines  

Chae, Byeong-Suk (College of Pharmacy, Woosuk University)
Shin, Tae-Yong (College of Pharmacy, Woosuk University)
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YAKHAK HOEJI / v.50, no.3, 2006 , pp. 184-190 More about this Journal
Abstract
We investigated the effect of fresh Rehmanniae radix methanol extracts (RGMeOH) on the in vitro production of cytokines by splenocytes and peritoneal macrophages isolated from C57BL/6 mice. Peritoneal macrophages and splenocytes were incubated with various concentrations of RGMeOH in the presence of $10\;{\mu}g/ml$ of lipopolysaccharide (LPS) $1\;{\mu}g/ml$ of concanavalin A (Con A) for cytokine assay, These results showed that RGMeOH remarkably attenuated LPS-increased production of $TNF-{\alpha}$ but not IL-6 by peritoneal macrophages and enhanced LPS-stimulated production of IL-10 in a dose-dependent manner RGMeOH significantly augmented the LPS- or Con A-stimulated production of IL-2 and $IFN-{\gamma}$ by splenocytes. These findings suggest that RGMeOH may attenuate inflammatory responses through down-regulation of $TNF-{\alpha}$ and up-regulation of IL-10, and that RGMeOH may up-regulate cell-mediated immune responses through increase in IL-2 and $IFN-{\gamma}$ production.
Keywords
Rehmanniae radix; $TNF-{\alpha}$; IL-6; IL-10; IL-2; $IFN-{\gamma}$;
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1 Tomoda, M., Miyamoto, H., Shimizu, N., Gonda, R. and Ohara, N. : Two acidic polysaccharides having reticuloendothelial system-potentiating activity from the raw root of Rehmanni glutinosa. Biol. Pharm. Bull. 17(11), 1456 (1994)   DOI   ScienceOn
2 Li, D. Q., Li, Y., Liu, Y., Bao, Y. M., Hu, B. and An, L. J. : Catalpol prevents the loss of CA1 hippocampal neurons and reduces working errors in gerbils after ischemia-reperfusion injury. Toxicon. 46(8), 845 (2005)   DOI   ScienceOn
3 Li, D. Q., Bao, Y. M., Zhao, J. J., Liu, C. P., Liu, Y. and An, L. J. : Neuroprotective properties of catalpol in transient global cerebral ischemia in gerbils: dose-response, therapeutic timewindow and long-term efficacy. Brain Res. 1029(2), 179 (2004)   DOI   ScienceOn
4 Kim, H. M., Lee, E. H., Lee, S. J., Shin, T. Y., Kim, Y. J. and Kim, J. B. : Effect of Rehmannia glutinosa on immediate type allergic reaction. Int. J. Immunopharmacol. 20, 231 (1998)   DOI   ScienceOn
5 Chen, L. Z., Feng, X. W. and Zhou, J. H. : Effects of Rehmannia glutinosa polysaccharide b on T-lymphocytes in mice bearing sarcoma 180. Zhongguo Yao Li Xue Bao. 16(4), 337 (1995)
6 Kubo, M., Asano, T., Matsuda, H., Yutani, S. and Honda, S. : Studies on Rehmanniae radix. III. The relation between changes of constituents and improvable effects on hemorheology with the processing of roots of Rehmannia glutinosa. Yakugaku Zasshi. 116(2), 158 (1996)   DOI
7 Abbas, A. K., Murphy, K. M. and Sher, A. : Functional diversity of helper T lymphocytes. Nature 383, 787 (1997)   DOI   ScienceOn
8 van Snick, J. : Interleukin-6: an overview. Annu. Rev. Immunol. 8, 253 (1990)   DOI   ScienceOn
9 Chai, Z., Gatti, S., Toniatti, C., Poli, V. and Bartfai, T. : Interleukin(IL)-6 gene expression in the central nervous system is necessary for fever response to lipopolysaccharide or IL-1$\beta$: a study on IL-6-deficient mice. J. Exp. Med. 183, 311 (1996)   DOI   ScienceOn
10 Andreakos, E., Sacre, S. M. , Smith, C., Lundberg, A., Kiriakidis, S., Stonehouse, T., Monaco, C., Feldmann, M. and Foxwell, B. M. : Distinct pathways of LPS-induced NF-kappa B activation and cytokine production in human myeloid and nonmyeloid cells defined by selective utilization of MyD88 and Mal/TIRAP. Blood. 103(6), 2229 (2004)   DOI   ScienceOn
11 Fiorentino, D. F., Zlotnik, A., Mosmann, T. R., Howard, M. and O'Garra, A. : IL-10 inhibits cytokine production by activated macrophages. J. Immunol. 147(11), 3815 (1991)
12 Fiorentino, D. F., Bond, M. W. and Mosmann, T. R. : Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones. J. Exp. Med. 170(6), 2081 (1989)   DOI   ScienceOn
13 Bogdan, C., Vodovotz, Y. and Nathan, C. : Macrophage deactivation by interleukin 10. J. Exp. Med. 174, 1549 (1991)   DOI   ScienceOn
14 Andersson, J., Nagy, S., Bjork, L., Abrams, J., Holm, S. and Andersson, U. : Bacterial toxin-induced cytokine production studied at the single cell level. Immunol. Rev. 127, 69 (1992)   DOI
15 Kubo, M., Asano, T., Shiomoto, H. and Matsuda, H. : Studies on rehmanniae radix. I. Effect of 50% ethanolic extract from steamed and dried rehmanniae radix on hemorheology in arthritic and thrombosic rats. Biol. Pharm. Bull. 17(9), 1282 (1994)   DOI   ScienceOn
16 Prieto, J. M., Recio, M. C., Giner, R. M., Manez, S., Giner- Larza, E. M. and Rios, J. L. : Influence of traditional Chinese anti-inflammatory medicinal plants on leukocyte and platelet functions. J. Pharm. Pharmacol. 55(9), 1275 (2003)   DOI   ScienceOn
17 Volk, H. D., Reinke, P. and Docke, W. D. : Balance between proand anti-inflammatory mediators Immunological monitoring of the inflammatory process: which variables- When to assess- Eur. J. Surg. 584, 70 (1999)
18 Lee, H. S., Kim, S. T. and Cho, D. K. : Effects of rehmanniae radix water extract on renal function and renin secretion rate in unanesthetized rabbits. Am. J. Chin. Med. 21(2), 179 (1993)
19 Munoz, C., Carlet, J., Fitting, C., Misset, B., Bleriot, J. P. and Cavaillon, J. M. : Dysregulation of in vitro cytokine production by monocytes during sepsis. J. Clin. Investig. 88, 1747 (1991)   DOI
20 Kim, H. M., An, C. S., Jung, K. Y., Choo, Y. K., Park, J. K. and Nam, S. Y. : Rehmannia glutinosa inhibits tumour necrosis factor-alpha and interleukin-1 secretion from mouse astrocytes. Pharmacol. Res. 40(2), 171 (1999)   DOI   ScienceOn
21 Diehl, S., Anguita, J., Hoffmeyer, A., Zapton, T., Ihle, J. N., Fikrig, E. and Rincon, M. : Inhibition of Th1 differentiation by IL-6 is mediated by SOCS1. Immunity. 13(6), 805 (2000)
22 Yokozawa, T., Kim, H. Y. and Yamabe, N. : Amelioration of diabetic nephropathy by dried Rehmanniae Radix (Di Huang) extract. Am. J. Chin. Med. 32(6), 829 (2004)   DOI   ScienceOn
23 Oh, K. O., Kim, S. W., Kim, J. Y., Ko, S. Y., Kim, H. M., Baek, J. H., Ryoo, H. M. and Kim, J. K. : Effect of Rehmannia glutinosa Libosch extracts on bone metabolism. Clin. Chim. Acta. 334(1-2), 185 (2003)   DOI
24 Kang, D. G., Sohn, E. J., Moon, M. K., Lee, Y. M. and Lee, H. S. : Rehmannia glutinose ameliorates renal function in the ischemia/reperfusion-induced acute renal failure rats. Biological & Pharmaceutical Bulletin. 28(9), 1662 (2005)   DOI   ScienceOn
25 Kiho, T., Watanabe, T., Nagai, K. and Ukai, S. : Hypoglycemic activity of polysaccharide fraction from rhizome of Rehmannia glutinosa Libosch. f. hueichingensis Hsiao and the effect on carbohydrate metabolism in normal mouse liver. Yakugaku Zasshi. 112(6), 393 (1992)