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Isolation and Carbohydrate Binding Specificity of a Lectin from the Hemolymph of Coastal Crab Macrophthalmus Japonicus  

Kim, Ha-Na (College of Pharmacy, Chung-Ang University)
Chung, Woo-Hyuk (College of Pharmacy, Chung-Ang University)
Bae, Chan-Hyung (College of Pharmacy, Chung-Ang University)
Hwang, Kwang-Woo (College of Pharmacy, Chung-Ang University)
Kim, Ha-Hyung (College of Pharmacy, Chung-Ang University)
Publication Information
YAKHAK HOEJI / v.50, no.3, 2006 , pp. 166-171 More about this Journal
Abstract
A calcium-dependent sialic acid-binding lectin has been isolated by thyroglobulin-affinity chromatography from the coastal crab Macrophthalmus Japonicus. This lectin, Macrophthalmus Japonicus lectin (MJL), was eluted with 50mM Tris-HCl, 0.3 M NaCl, 10 mM EDTA, and the recovery yield from the crude protein extract was about 5.6%. The molecular weight of MJL was estimated as 65 kDa in SDS-PAGE both under reducing and non-reducing conditions. MJL induced an agglutination reaction in rabbit, rat, and mouse erythrocytes, but not in human ABO types. This activity was effectively inhibited by sialoglycoproteins such as fetuin, bovine submaxillary mucin, and thyroglobulin.
Keywords
lenctin; crab; sialoglycoprotein;
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