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Evaluation of a Streptococcus pneumoniae DNA Vaccine Efficacy  

Lee Jue-Hee (College of Pharmacy, Dongduk Women's University)
Han Yongmoon (College of Pharmacy, Dongduk Women's University)
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YAKHAK HOEJI / v.49, no.6, 2005 , pp. 484-489 More about this Journal
Abstract
Streptococcus pmeumoniae is the leading cause of pneumonia and bacterial meningitis. The current polysaccharide vaccine has been reported ineffective in elderly adults and children less than 2 years of age. Thus, in recent many researchers have been focused on a different approach, DNA vaccine. In our laboratory we developed a Streptococcus pneumoniae DNA (SPDNA) vaccine. This SPDNA vaccine was formulated by inserting the region encoding part of the capsule in the S. pneumoniae into the LAMP-1. In present work, with use of the SPDNA vaccine we attempted to establish a certain methodology useful for evaluation of effectiveness and immunoresponse of a DNA vaccine. Results showed that the subcutaneous route was the most effective for production of antisera specific for S. pneumoniae in mice. By isotyping analyses, IgM, IgGl, IgG2a, and IgG2b were determined. In addition, INF-$\gamma$ and IL-4 were predominantly detected. Combination of those data resulted in a pattern of IgGl < IgG2a=IgG2b and INF$\gamma\>$ >IL-4, which indicates the inmmunity towards the Thl response predominantly; furthermore, the SPDNA vaccination induced resistance of the CD4+T lymphocyte-depleted mice against disseminated pneumococcal infection. These data appear to be possibly due to activation of CDS8+T cell-activation. Taken together, this methodology can be applied for evaluating efficacy and mode of action of a DNA vaccine as minimum critera.
Keywords
DNA vaccine; S. pneumoniae; isotyping; cytokine; mode of action; evaluation;
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1 Ogunniyi, A. D., Giammarinaro, P. and Paton, J. C. : The genes encoding virulence-associated proteins and the capsule of Streptococcus pneumoniae are upregulated and differentially expressed in vivo. Microbiology 148, 2045 (2001)
2 Pengo, V., Biasiolo, A., Pegoraro, C., Cucchini, U., Noventa, F. and lliceto, S. : Antibody profiles for the diagnosis of antiphospholipid syndrome. Thromb. Haemost. 93(6), 1147 (2005)
3 Guy, B. and Burdin, N. : New adjuvants for parenteral and mucosal vaccines. Therapie. 60(3), 235 (Review) (2005)   DOI   ScienceOn
4 Bosarge, J. R., Watt, J. M., McDaniel, D. O., Swiatlo, E. and McDaniel, L. S. : Genetic immunization with the region encoding the alpha-helical domain of PspA elicits protective immunity against Streptococcus pneumoniae. Infect. Immun. 69(9), 5456 (2001)   DOI   ScienceOn
5 Endo, T., Sato, N., Koizumi, K., Nishio, M., Fujimoto, K., Yamamoto, S., Sakai, T., Bohgaki, T., Sawada, K. and Koike, T. : A preliminary analysis of the balance between Th1 and Th2 cells after CD34+ cell-selected autologous PBSC transplantation. Cytotherapy 6(4), 337 (2004)   DOI   ScienceOn
6 Han, Y. and Lee, J.-H. : A pneomococcal conjugate vaccine formula induces protection in mice against disseminated disease due to Streptococcus pneumoniae. Yakhak Hoeji 48, 345 (2004)
7 Lesinski, G. B., Smithson, S. L., Srivastava, N., Chen, D., Widera, G. and Westerink, M. A. : A DNA vaccine encoding a peptide mimic of Streptococcus pneumoniae serotype 4 capsular polysaccharide induces specific anti-carbohydrate antibodies in Balb/c mice. Vaccine. 19, 1717 (2001)   DOI   ScienceOn
8 Lee, J.-H. and Han, Y. : Antibody induced by the JY-Pol pneumococcal conjugate protects mice against systemic infection due to Streptococcus pneumoniae. Yakhak Hoeji 48, 369 (2004)
9 Faubel, S., Ljubanovic, D., Poole, B., Dursun, B., He, Z., Cushing, S., Somerset, H., Gill, R. G. and Edelstein, C. L. : Peripheral CD4 T-cell depletion is not sufficient to prevent ischemic acute renal failure. Transplantation 80(5), 643 (2005)   DOI   ScienceOn
10 Smith, H. A. and Klinman, D. M. : The regulation of DNA vaccines. (Review). Curr. Opin. Biotechnol. 12(3), 299 (2001)   DOI   ScienceOn
11 Miyaji, E. N., Dias, W. O., Gamberini, M., Gebara, V. C., Schenkman, R. P., Wild, J., Riedl, P., Reimann, J., Schirmbeck, R. and Leite, L. C. : PsaA (pneumococcal surface adhesin A) and PspA (pneumococcal surface protein A) DNA vaccines induce humoral and cellular immune responses against Streptococcus pneumoniae. Vaccine. 20, 805 (2001)   DOI   ScienceOn
12 Montgomery, D. L., Ulmer, J. B., Donnelly, J. J. and Liu, M. A. : DNA vaccines. (Review). Pharmacol. Ther. 74(2), 195 (1997)   DOI   ScienceOn
13 Donnelly, J. J., Ulmer, J. B. and Liu, M. A. : DNA vaccines. Dev. BioI. Stand. 95, 43 (1998)
14 Donnelly, J. J., Ulmer, J. B., Shiver, J. W. and Liu, M. A. : DNA vaccines. Ann. Rev. Immunol. 15, 617 (1997)   DOI   ScienceOn
15 Nichols, W. W., Ledwith, B. J., Manam, S. V. and Troilo, P. J. : DNA vaccine integration into host cell genome. Ann. N. Y. Acad Sci. 72, 30 (1985)
16 Ellenberg, S. S. and Chen, R. T. : The complicated task of monitoring vaccine safety. Public Health Rep. 112, 10 (1997)
17 Klinman, D. M., Takeshita, F., Kamstrup, S., Takeshita, S., Ishii, K., Ichino, M. and Yamada, H. : DNA vaccines: capacity to induce auto-immunity and tolerance. Dev. Biol. (Basel). 104, 45 (2000)
18 Lesinski, G. B. and Westerink, M. A. : Vaccines against polysaccharide antigens. Curr Drug. Targets. Infect. Disord. 1, 325 (2001)   DOI   ScienceOn
19 Cichutek, K. : DNA vaccines: development, standardization and regulation. Intervirology 43, 331 (2000)   DOI   ScienceOn
20 Gregory, B., Lesinski, S., Smithson, L., Srivastava, N., Chen, D., Widera, G. and Westerink, J. : A DNA vaccine encoding a peptide mimic of Streptococcus pneumoniae serotype 4 capsular polysaccharide induces specific anti-carbohydrate antibodies in Balblc mice. Vaccine. 19, 1717 (2001)   DOI   ScienceOn
21 Wang, D., Christopher, M. E., Nagata, L. P., Zabielski, M. A., Li, H., Wong, J. P. and Samuel, J. : Intranasal immunization with liposome-encapsulated plasmid DNA encoding influenza virus hemagglutinin elicits mucosal, cellular and humoral immune responses. J. Clin. Viral. 31(Suppl 1), S99 (2004)   DOI   ScienceOn