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Effect of Campsis grandiflora on Antioxidative Activity in UVB-irradiated Human Dermal Fibroblasts  

Kim, Jin-Hwa (Hanbul Cosmetics, R & D Center)
Lee, Bum-Chun (Hanbul Cosmetics, R & D Center)
Zhang, Yong-He (Department Of Pharmacolgy, School of Basic Medical Science, Peking University)
Pyo, Hyeong-Bae (Hanbul Cosmetics, R & D Center)
Publication Information
YAKHAK HOEJI / v.49, no.2, 2005 , pp. 174-179 More about this Journal
Abstract
The human skin is constantly exposed to environmental irritants such as ultraviolet, smoke, chemicals. Free radicals and reactive oxygen species (ROS) caused by these environmen tal facts play critical roles in cellular damage. These irritants are in themselves damaging to the skin structure but they also participate the immensely complex inflammatory reaction. The purpose of this study was to investigate the skin cell protective effect of Campsis grandiflora extract on the UVB-irradiated human dermal fibroblasts (HDFs). We tested free radical and superoxide scavenging effect in vitro. C. grandiflora extracts had potent radical scavenging effect by 82% at $100{\mu}g/ml$, respectively. For testing intracellular ROS scavenging activity the cultured HDFs were analyzed by increase in DCF fluorescence upon exposure to UVB 20 $MJ/cm^2$ after treatment of C.grandiflora extracts. The results showed that oxidation of CM-DCFDA was inhibited by C.grandiflora extracts effectively and C.grandiflora extracts has a potent free radical scavenging activity in UVB- irradiated HDFs. In ROS imaging using confocal microscope we visualized DCF fluorescence in HDFs directly. In conclusion, our results suggest that C.grandiflora can be effectively used for the prevention of UV-induced adverse skin reactions such as radical production, and skin cell damage.
Keywords
Campsis grandiflora; fibroblast; anti-oxidation; free radical scavenging; UVB;
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