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Antibody Induced by the JY-Pol Pneumococcal Conjugate Protects Mice Against systemic Infection Due to Streptococcus pneumoniae  

Lee, Jue-Hee (College of Pharmacy, Dongduk Women's University)
Han, Yong-Moon (College of Pharmacy, Dongduk Women's University)
Publication Information
YAKHAK HOEJI / v.48, no.6, 2004 , pp. 369-373 More about this Journal
Abstract
We previously reported that Streptococcus pneumoniae capsule attached to the surface protein (JY-Pol) was protective to systemic pneumococcal infection. The JY -Pol antigen induced IgM, IgG, and IgA in mice and provoked cell-mediated immunity. In this current study, we investigated the effect of anti JY-Pol antiserun and monoclonal antibody C2 (Mab C2) specific for the JY-Pol antigen against the pneumococcal disease. Mice that were given the antiserum survived longer than mice that received antiserum pre-absorbed with S.pneumoniae cells or DPBS as a negative control. Heat-treated anti JY-Pol antiserum resulted in survival rates similar to intact fresh JY-Pol antiserum. Mab C2 isolated from JY-Pol-immunized mice also enhanced resistance of naive mice against the pneumococcal diseaser. This protection by Mab C2 appeared to be mediated by opsonization as determined in a RAW 264.7 monocyte/macrophage cell line. Epitope analysis showed that Mab C2 epitope consisted of glucuronic acid and glucose that blocked the interaction of JY-Pol to the C2. Taken together, these data indicate that the antiserum induced by the JY-Pol, a naturally pneumococcal conjugate formula, mediated the protection by passive transfer, which was confirmed by protective effect of Mab C2.
Keywords
S. pneumoniae; JY-Pol; antiserum; opsonization; epitope; mice;
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1 Dagan, R. : Pneumococcal conjugate vaccines : The potential to alter antibiotic use and nasopharyngeal carriage. Curr. Ther. Res. Clin Exp. 63, A22 (2002)
2 MacLeod, C. M. and Krauss, M. R. : Relation of virulence of pneumococcal strains for mice to the quantity of capsular polysaccharide formed in vitro. J. Exp. Med. 92, 1 (1950)   DOI   ScienceOn
3 Han, Y. and Cutler, J. E. : Assessment of a mouse model of neutropenia and the effect of an anti-candidiasis monoclonal antibody in these animals. J. Infect. Dis. 175, 1169 (1997)   DOI   ScienceOn
4 Han, Y., Jin , B. S., Ko, S. K. and Lee, J.-H. Immunoactivity of ginsenosides re and rg1 that enhances resistance of mice against experimental disseminated candidiasis. Nat. Prod. Sci. 10, 134 (2004)
5 Areas, A. P., Oliveira, M. L., Miyaji, E. N., Leite , L. C., Aires, K. A., Dias, W. O. and Ho, P. L. : Expression and characterization of cholera toxin B-pneumococcal surface adhesin A fusion protein in Escherichia coli: ability of CTBP-PsaA to induce humoral immune response in mice. Biochem. Biophys. Res. Commun. 13, 192 (2004)
6 Mor, G. : Plasmid DNA : A new era in vaccinology. Biochem. Pharmacol. 55, 1151 (1998)
7 Kim, J. O. and Weiser, J. N. : Association of intrastrain phase variation in quantity of capsular polysaccharide and teichoic acid with the virulence of Streptoeoccus pneumoniae. J. Infect. Dis. 177, 368 (1988)   DOI   ScienceOn
8 Lesinski, G. B. and Westerink, M. A. : Vaccines against polysaccharide antigens. Curr. Drug. Targets. Infect. Disord. 1, 325 (2001)   DOI   ScienceOn
9 Mitchell, T. J. : Virulence factors and the pathogenesis of disease caused by Streptococcus pneumoniiae. Res. Microbiol. 151, 419 (2000)
10 Lesinski, G. B., Smithson, S. L., Srivastava, N., Chen, D., Widera, G. and Westerink, M. A. J.: A DNA vaccine encoding a peptide mimic of Streptoecoccus peumoniae serotype 4 capsular polysaccharide induces specific anti-carbohydrate antibodies in Balb/c mice. Vaccine 19, 1717 (2001)   DOI   ScienceOn
11 Richter, M. Y., Jakobsen, H., Birgisdottir, A., Haeuw, J. F., Power, U. F., Del Giudice, G., Bartoloni, A. and Jonsdottir, I.: Immunization of female mice with glycoconjugates protects their offspring against encapsulated bacteria. Infect. Immun. 72, 187 (2004)   DOI   ScienceOn
12 Bosarge, J. R., Watt, J. M., McDaniel, D. O., Swiatlo, E. and McDaniel, L. S. : Genetic immunization with the region encoding the alpha-helical domain of PspA elicits protective immunity against Streptococcus pneumoniae. Infect. Immun. 69, 5456 (2001)   DOI   ScienceOn
13 Pelton, S. I., Dagan, R., Gaines, B. M., Klugman, K. P., Laufer, D.,O'Brien, K. and Schmitt, H. J. : Pneumococcal conjugate vaccines : proceedings from an Interactive Symposium at the 41st Interscience Conference on Antimicrobial Agents and Chemotherapy. Vaccine 21, 562 (2003)   DOI   ScienceOn
14 How, M. J., Brimacombe, J. S. and Stacey, M. : The pnemococcal polysaccharides. Adv. Carbohyd. Chem. 19, 303 (1964)   PUBMED
15 Ogunniyi, A. D., Giammarinaro, P. and Paton, J. C. : The genes encoding virulence-associated proteins and the capsule of Streptococcus pneumoniae are upregulated and differentially expressed in vivo. Microbiology 148, 2045 (2002)   DOI   PUBMED
16 Gregory, B., Lesinski, S., Smithson, L., Srivastava, N., Chen, D., Widera, G. and Westerink, J. : A DNA vaccine encoding a peptide mimic of Streptococcus pneumoniae serotype 4 capsular polysaccharide induces specifec anti-carbohydrate antibodies in Balb/c mice. Vaccine 19, 1717 (2001)   DOI   ScienceOn
17 Magee, A. D. and Yother, J. : Requirement for capsule in colonization by Streptococcus pneumoniae. Infect. Immun. 69, 3755 (2001)   DOI   ScienceOn
18 Smith, G. P. and Scott, J. K. : Peptide-display phage libraries. Methods Enzymol. 217, 228 (1992)   DOI
19 Jedrzejas, M. J. : Pneumococcal virulence factors : Structure and function. Microbio. Mol. Biol. Rev. 65, 187 (2001)
20 Heidelberger, M., Kendall, F. E. and Scherp, H. W. : The specific ploysaccharide of Type I, II, and III pneumococcus : A revision of method and data. J. Exp. Med. 64, 559 (1936)
21 Han, Y. and Cutler, J. E. : Antibody response that protects against disseminated candidiasis. Infect. Immun. 63, 2714 (1995)
22 Robbins, J. B., Austrian, R., Lee, C.-J., Rastogi, S. C., Schiffman, G., Henrichsen, J., et al. : Considerations for formulating the second generation pneumococcal capsular polysaccharide vaccine with emphasis on the cross-reactive types within groups. J. Infet. Dis. 148, 1136 (1983)
23 Malley, R., Morse, S. C., Leite, L. C., Areas, A. P., Ho, P. L., Kubrusly, F. S., Almeida, I. C. and Anderson, P. : Multiserotype protection of mice against pneumococcal colonization of the nasopharynx and middle ear by killed nonencapsulated cells given intranasally with a nontoxic adjuvant. Infect. Immun. 2004 72, 4290 (2004)
24 Tuomanen, E. I. and Masure, R. H. : Molecular and cellular biology of pneumococcal infection. In : Streptococcus pneumoniae molecular biology and mechanism of disease. Mary Ann Liebert, New York, 295 (2000)
25 Han, Y. and LJH. : A pneumococcal conjugate vaccine formula induces protection in mice against disseminated disease due to Streptococcus pneumoniae. Yak Hak Hoe Ji. (in press) (2004)