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Effect of Melothria heterophylla Extract on Expression of Matrix Metalloproteinase-1 in Human Skin Fibroblasts  

Cho , Young-Ho (R&D Center, Hanbul Cosmetics Corporation)
Sim , Gwan-Sub (R&D Center, Hanbul Cosmetics Corporation)
Kim , Jin-Hui (R&D Center, Hanbul Cosmetics Corporation)
Park , Sung-Min (R&D Center, Hanbul Cosmetics Corporation)
Lee , Bum-Chun (R&D Center, Hanbul Cosmetics Corporation)
Pyo , Hyeong-Bae (R&D Center, Hanbul Cosmetics Corporation)
Yun , Yeo-Pyo (College of Pharmacy, Chungbuk National University)
Park , Hum-Dai (Department of Biotechnology, College of Engineering, Daegu University)
Publication Information
YAKHAK HOEJI / v.48, no.6, 2004 , pp. 358-363 More about this Journal
Abstract
Matrix metalloproteinases (MMPs) are known to play an important role in photoaging by mediating the degradation of extracellular matrix proteins. In this study, to develop a n ew anti-aging agent, we investigated the antioxidant activity and the inhibitory effect of Melothria heterophylla extract on expression of MMP-1 in UVA-irradiated human dermal fibroblasts and MMP-1 activity. The M.heterophylla extract was found to scavenge radicals and reactive oxygen species (ROS) with the $SC_{50}$ values of $13{\mu}g/ml$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and $20{\mu}g/ml$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. UVA-induced MMP-1 expression was reduced about 80% by $100{\mu}g/ml$ of the M.heterophylla extract but MMP-1 Mrna expression was not inhibited. Therefore, we conclude that the M.heterophylla extract significantly inhibits MMP-1 expression at the protein level. Also, the M.heterophylla extract inhibited MMP-1 activity in a dose dependent manner. From these results, we suggest that the M.heterophylla extract can be used as a new anti-aging agent by antioxidant activity, regulation of UVA-induced MMP-1 production, and inhibition of MMP-1 activity.
Keywords
Melothria heterophylla; antioxidant; MMP;
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