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Immunoactivities of PVMP, a Protein-polysaccharide Fraction Isolated from Mycelial Culture of Psathyrella velutina  

정경수 (충남대학교 약학대학 미생물면역학교실)
이지선 (충남대학교 약학대학 미생물면역학교실)
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YAKHAK HOEJI / v.48, no.5, 2004 , pp. 261-265 More about this Journal
Abstract
In the previous report, we described the marked antitumor and immunomodulatory activities of PVp, a protein-polysaccharide fraction of a Korean wild mushroom Psathyrella velutina. In this study, a protein-polysaccharide fraction, PVMP, was prepared from the shake-cultured mycelia of the same mushroom and its immunoactivities as well as chemical compositions were investigated. At 200 $\mu\textrm{g}$/ml, PVMP weakly stimulated the BALB/c mouse splenic lymphocytes to form lymphoblasts and upregulated the expression of CD25 molecules, but failed to stimulate peritoneal macrophages. In chemical analysis these two protein-polysaccharide fractions were found to be quite different in that the carbohydrate contents of PVMP and PVP, respectively, was 85.3% and 41.2%. These results reveals that PVMP, unlike PVP, is a moderate immunostimulator on the immune system.
Keywords
Psathyrella velutina; mycelium; PVMP; protein-polysaccharide; lymphoblast; CD25; immunostimulation;
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1 Komatsu, N., Okubo, S., Likumoto, S., Kimura, K., Saito, G. and Sasaki, S. : Host-mediated antitumor action of schizophyllan, a glucan produced by Schizophyllum commune. Gann 60, 137 (1969)   PUBMED
2 Tsukagoshi, S. and Ohashi, F. : Protein bound polysaccharide preparation, PS-K, effective against mouse sarcoma 180 and rat ascites hepatoma by oral use. Gann. 65, 557 (1974)
3 Chung, K. S., Kim, S. S., Kim, H. S., Han, M. W. and Kim, B. K. : Antitumor activity of Kp, a protein-polysaccharide from the mycelial culture of Phellinus linteus. Yakhak Hoeji 38, 158 (1994)
4 Lee, J. S., Lee, I. S., Chung, K. S., Kim, Y. H., Han, Y. H. and Lee, M. H. : Flow cytometrical investigation on antitumor activity of mycelial culture of insect -born fungus Paecilomyces japonica DGUM 32001. Yakhak Hoeji 45, 64 (2001)
5 Norris, J. R. and Ribbons, D. W. : Methods in microbiology Vol. 5B, Academic Press, New York, p. 209 (1971)
6 Guillot, J. and Konska, G. : Lectins in higher fungi. Biochemical Systematics and Ecology 25, 203 (1997)   DOI   ScienceOn
7 Carlile, M. J. and Watkinson, S. C. : The Fungi, Academic Press, New York, p. 52 (1994)
8 Chung,K.S., Kim, S. S., Kim, H. S., Kim, K. Y., Han, M. W. and Kim, B. K. : Effect of Kp, an antitumor protein-polysaccharide from mycelial culture of Phellinus linteus on the humoral immune response of tumor-bearing ICR mice to sheep red blood cells. Arch. Pharm. Res. 16, 336 (1993)   DOI   ScienceOn
9 Stewart, C. C. and Stewart, S. J. : Cell preapration for the identification of leukocytes. In 'Methods in Cell Biology. Vol. 41. Flow Cytometry (2nd ed.), part A' by Darzynkiewicz, Z., Robbinson, J. P. and Crissman, H. A. (eds.). Academic Press, San Diego p. 39 (1984)
10 Musilek, M., Ticha, M., Volc, J. and Kocourek, J. : Studies on lectins. LXXI. Lectins in mycelial cultures of Kuehneromyces mutabilis, Pholiota, and Flammulina velutipes. : In 'Lectins Biology, Biochemistry, Clinical Biochemistry (Vol. 7)' (Kocourek, J. and Freed, D. L. J., eds). Sigma Chemical Company, St. Louis p. 53 (1990)
11 Borchers, A. T., Keen, C. L. and Gershwin, M. E.: Mushrooms, tumors, and immunity. An update. Exp. Biol.Med. 229, 393 (2004)
12 Chihara, G., Hamuro, J., Maeda, Y. Y., Arai, Y. and Fukuoka, F. : Fraction of the polysaccharides with marked antitumor activity, especially lentinan, from Lentinus edodes (an edible mushroom). Cancer Res. 30, 2776 (1970)
13 Chung, K. S. and Lee, J. S. : Antitumor immunomodulatory activity of PVP, a protein-polysaccharide fraction prepared from a wild mushroom Psathyrella velutina. Yakhak Hoeji 45, 617 (2001)
14 Oh, J. Y. and Chung, K. S. : Flow cytometrical analysis of the antitumor and immunomodulatory activities of GLB-A and GLB-B, the protein-polysaccharide fractions of the growing tips of Ganoderma lucidum. Yakhak Hoeji 42, 487 (1998)
15 Cho, K. J. and Chung, K. S. : Antitumor and antileukopenic activity of HCA,the protein-polysaccharide fraction of cultured mycelia of Hebeloma crustuliniforme. Yakhak Hoeji 43, 629 (1999)
16 Borchers, A. T, Stern, J. S., Hackman, R. M., Keen, C. L. and Gershwin, M. E. : Mushrooms, tumors, and immunity. Proc. Soc. Exp. BioI. Med. 221, 281 (1999)   DOI   ScienceOn
17 Chung, K.S. and Kim, J. H. : Antitumor and immunomodulatory activity of Lycoperdon pedicellatum. Yakhak Hoeji 44, 463 (2000)
18 Moon, C. K., Lee , S. H., Mock, M. S. and Kim, D. O. : Antitumor activity of the polysaccharide-fraction (Copolang) from Coriolus versicolor and its effects on the immune function. Yakhak Hoeji 31, 126 (1987)
19 Ohno, N., Suzuki, I, Oikawa, S., Sato, K., Miyazaki, T. and Yadomae, T. : Antitumor activity and structural characterization of glucans extracted from cultued fruit bodies of Grifola frondosa. Chem. Pharm. Bull. 32, 1142 (1984)
20 Chung, K. S., Kim, S. B. and Chung, S. H. : Immunoactivities of the protein-polysaccharides of the tips of the growing car pophores of Ganoderma lucidum. Yakhak Hoeji 41, 105 (1997)